Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
  • Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
  • Keyword register: select an initial character and browse associated keywords
  • Search by CBS edition: Select a CBS edition and find all related publications

Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. You can export your saved items to PDF by clicking the download icon.

      129 064
      Effect-directed profiling and identification of bioactive metabolites from field, in vitro-grown and acclimatized Musa spp. accessions using high-performance thin-layer chromatography-mass spectrometry
      I.O. AYOOLA-ORESANYA, M.A. SONIBAREA, B. GUEYEB, R. PALIWALB, M.T. ABBERTON, Gertrud E. MORLOCK* (*Institute of Nutritional Science, and Interdisciplinary Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University Giessen, Giessen, Germany;

      J Chromatogr A, 1616, 460774 (2020). Methanolic extracts of leaves of Musa acuminata, M. balbisiana and M. sapientum (Musaceae), either from fields or from in vitro cultures or from the plantlets derived from in vitro culture and acclimatized in isolated warm room, were separated on HPTLC silica gel layers with toluene – ethyl acetate – methanol 6:3:1 or ethyl acetate – toluene – formic acid – water 34:5:7:5. When intended for MS experiments, layers were previously washed twice with methanol – formic acid 10:1, once with acetonitrile – methanol 2:1 and air-dried. Evaluation under white light, UV 254 nm and 366 nm. Derivatization by immersion (2s, 2cm/s) into natural product reagent preceded by heating at 110 °C for 5 min, or into anisaldehyde sulfuric acid reagent, diphenylamine aniline reagent, ninhydrin reagent, followed by the same heating procedure. Besides, plates were neutralized by cold air stream followed with phosphate buffer (8 %, pH 7.5) piezoelectrically sprayed on the plates and automated plate drying. Thereafter, 9 effect-directed assays (EDA) were performed for free radical (DPPH•) scavengers, for enzymatic inhibitors (α-amylase, acetyl- and butyryl-cholinesterase, α- and β-glucosidase), for antimicrobial compounds (Gram-positive Bacillus subtilis assay, Gram-negative Aliivibrio fischeri bioluminescence assay), and for mutagenic compounds (SOS response – UMU-C test using Salmonella typhimurium suspension and 4-nitroquinoline 1-oxide as positive control). The bands of 4 active compounds were eluted with methanol through a TLC-MS interface pump into a quadrupole-Orbitrap mass spectrometer. Full scan mass spectra (m/z 50−800) in the positive and negative ionization modes were recorded using electrospray ionization (ESI, spray voltage 3.3kV, capillary temperature 320°C, collision energy 35 eV). By comparison to a standard, one band present in all samples was identified as linolenic acid. For the other bands, only present in in vitro grown accessions, only raw molecular formulas and phytochemical classes were assigned (a pyrrolidine alkaloid, an amino-acid, a phenolic derivative).

      Classification: 4e, 7, 11a, 18a, 22, 32e
      128 043
      Nutritional and phytochemical profiling of nutracereal finger millet (Eleusine coracana L.) genotypes
      U. NAKARANI, D. SINGH*, K. SUTHAR, N. KARMAKAR, P. FALDU, H. PATIL (*Department of Plant Molecular Biology and Biotechnology, ACHF, NAU, Navsari, Gujarat 396 450, India,

      Food Chem. 341, 128271 (2021). HPTLC of finger millet (Eleusine coracana L.) on silica gel with n-butanol - water - acetic acid 4:1:1. Detection by spraying with ninhydrin, followed by heating at 100 °C. Amino acid profiling under UV light at 546 nm. The hRF values for 21 standard amino acids ranged from 2 to 51.

      Classification: 18a
      127 052
      Pressurized planar electrochromatography of DNS amino acids derivatives in silica gel and silanized silica gel systems with formic acid addition to the water mobile phase
      A. CHOMICKI*, T. DZIDO (*Department of Physical Chemistry, Medical University of Lublin, Chodźki 4a Str., 20-098 Lublin, Poland,

      J. Planar Chromatogr. 34, 105-111 (2021). HPTLC of dansyl derivatives of amino acids on silica gel (1) and RP-18 (2) with acetonitrile in the concentration range from 0 to 40 % in water - formic acid solution for (1) and acetonitrile in the concentration range from 10 to 85 % in water - formic acid solution for (2). Pressurized planar electrochromatography (PPEC) under the same conditions with polarization voltage 0.500 kV and separation time of 15 min. Detection under UV light. The separation selectivity is different between HPTLC and PPEC due to the electrophoretic effect in PPEC.

      Classification: 18a
      127 002
      Low temperature plasma probe mass spectrometry for analytes separated on thin-layer chromatography plates: direct vs. laser assisted desorption.
      X. GONG, D. ZHANG, I. B. EMBILE, Y. SHE, S. SHI, G. GAMEZ* (*Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas, USA;

      J Am Soc Mass Spectrom 31(9), 1981-1993 (2020). Low-temperature plasma-mass spectrometry was studied for comparison between direct desorption (DD) and diode laser assisted desorption (LD) in terms of quantitative and qualitative analysis of compounds from cellulose vs. silica gel TLC layers. Compounds (the 20 common amino acids, propofol, nicotine, cotinine, salicylamide, acetylsalicylic acid, paracetamol, caffeine, valprolactone and its isomer 4-ene-valproic acid) were applied on the TLC plates (without development) at different concentrations; a commercial mixture of acetylsalicylic acid, paracetamol and caffeine was also applied on TLC plates, developed with dichloromethane – ethyl acetate 1:50, detection at UV 254 nm and quantitative MS. In general, DD provided good results on cellulose, where LODs where between 0.01 and 2.55 ng/mm2, whereas several compounds remained undetected on silica gel. LD however provided LODs on silica gel from 0.3 to 84 pg/mm2. Tandem MS with collision-induced dissociation was implemented to improve signals, LODs and to characterize the other analytical figures-of-merits (including detection of the main fragment ions, determination of optimal laser beam width and irradiance depending on the compounds). For the two metabolites of valproic acid, the ions and fragments had identical values; therefore, a mix of the two isomers had to be applied and separated with dichloromethane – methanol 50:1 before MS; one half of the plate was visualized for control by dipping into potassium permanganate reagent (7.5g KMnO4, 50g K2CO3, 0.75g NaOH in 1L water).

      Classification: 4e, 7, 8b, 11a, 18a, 22
      126 035
      Structure–retardation factor relationship of natural amino acids in two different mobile phases of RP-TLC
      S. YOUSEFINEJAD*, F. HONARASA, S. AKBARI, M. NEKOEINIA (*Research Center for Health Sciences, Department of Occupational Health Engineering, Institute of Health, School of Health, Shiraz University of Medical Sciences, Shiraz 71645, Iran,

      J. Liq. Chromatogr. Relat. Technol. 43, 580-588 (2020). Retardation factor (hRF) of 42 amino acids in two different eluents (acetonitrile - sodium azide and 1,2 dioxane-sodium azide solutions) were predicted by different quantitative structure-retention relationship (QSRR) methods. The method analyzed the effect of sum of geometrical distances between N and O in separation of amino acids in RP-TLC.

      Classification: 2c, 18a
      100 043
      Indirekt TLC resolution of amino acid enantiomers after derivatization with Marfey’s reagent and its chiral variants
      R. BHUSHAN*, H. BRÜCKNER, V. KUMAR, D. GUPTA (*Department of Chemistry, Indian Institute of Technology, Roorkee247 667, India;

      J. Planar Chromatogr. 20, 165-171 (2007). TLC of 17 DL amino acids derivatized with1-fluoro-2,4-dinitrophenyl-5-L-alaninamide, 1-fluoro-2,4-dinitrophenyl-5-L-phenylalaninamide, or 1-fluoro-2,4-dinitrophenyl-5-L-valinamide on silica gel with phenol - water 3:1 or on RP-18 with mobile phases containing acetonitrile and triethylamine-phosphate buffer (50 mM, pH 5.5) with saturation for 10-15 min.

      Classification: 18a
      105 087
      Simultaneous determination of irbesartan and hydrochlorothiazide by HPTLC method
      B. MEHTA*, S. MORGE (*Dept. of Chem. University of Mumbai, Santacruz (E), Mumbai, 400098, India)

      Indian Drugs 47(2), 71-74 (2010). HPTLC of irbesartan and hydrochlorothiazide on silica gel with acetone - chloroform - ethyl acetate - methanol 6:6:6:1. The plates were preconditioned for 10 min in a saturated chamber prior to development. The hRf value of irbesartan was 27 and of hydrochlorothiazide 37. The linearity was 1500-9000 ng/band and 125-750 ng/band for irbesartan and hydrochlorothiazide respectively. The average recovery for both drugs was 99.4-99.5 %.

      Classification: 17c, 18a, 32a
      113 041
      On-plate identification of amino acids with preliminary separation using green solvents
      A. MOHAMMAD, A. SIDDIQ, A. MOHEMAN, G. EL-DESOKY* (*Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh, India,

      J. Liq. Chromatogr. Relat. Technol. 37, 829-840 (2014). HPTLC of amino acids such as leucine (1), isoleucine (2), phenylalanine (3), tyrosine (4), alanine (5), lysine (6), proline (7), serine (8), glutamic acid (9), methionine (10), arginine (11), histidine (12) and tryptophan (13) on silica gel with n-butyl acetate – n-butyl alcohol – ethylene glycol 3:5:2. Detection by spraying with ninhydrin (0.3 % in acetone). The hRF values for amino acids (1) to (13) ranged between 9 and 74. The LODs for (5) and (6) were 100 ng/zone and 60 ng/zone, respectively.

      Classification: 18a