Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      128 093
      High-throughput enzyme inhibition screening of 44 Iranian medicinal plants via piezoelectric spraying of planar cholinesterase assays
      E. AZADNIYA, I. THOMÄ, J. BAAKE, Gertrud E. MORLOCK* (*Institute of Nutritional Science, and TransMIT Center for Effect-Directed Analysis, Justus Liebig University Giessen, Giessen, Germany;

      Journal of Chromatography B, 1184, 122956 (2021). Test for acetyl- and butyrylcholinesterase (AChE and BChE) inhibition without development of piperin (standard inhibitor of AChE and BChE) and ethanol – water (3:2) extracts of Iranian plants, on HPTLC silica gel prewashed twice with methanol – water 3:2 and dried 60 min at 120°C. After sample application the plate was immersed (speed 3.5 cm/s, time 2 s) into enzyme solution (6.6 units/mL AChE or 3.3 units/mL BChE in TRIS buffer 0.05 M, with bovine serum albumin 0.1 %, pH 7.8), incubation 25 min at 37°C and immersion (speed 3.5 cm/s, time 1 s) into chromogenic substrate solution (α-naphthyl acetate 0.1 % and Fast Blue salt B 0.2 % in ethanol – water, 1:2). Seven mobile phases were tested for the active samples. Best separation was obtained with toluene – ethyl acetate – formic acid – water 4:16:3:2 and with toluene – ethyl acetate – methanol 6:3:1. Before enzymatic assay, plates developed with acidic mobile phases were neutralized by spraying 3 mL citrate phosphate buffer (Na2HPO4 8 %, citric acid q.s. ad pH 7.5) followed by 10 min of automatic drying. Enzymatic assay was performed using a piezoelectric spraying device: a) pre-wetting by spraying 1 mL TRIS buffer (0.05 M, pH 7.8); b) spraying 3 mL of the enzyme solution; c) incubation 25 min in a humid box at 37°C; d) spraying 0.5 mL substrate solution; e) 5 min drying at room temperature, and then 10 min of automatic drying. By spraying, zone shift and zone diffusion, which occurred with plate immersion, were avoided. For development control, derivatization was done by piezoelectrically spraying 4 mL of sulfuric anisaldehyde reagent (anisaldehyde – sulfuric acid – acetic acid – methanol, 1:10:20:170), followed by heating 3 min at 110°C. For identification of zones of interest, direct elution with methanol from underivatized HPTLC plates through a TLC-MS interface directly to a MS. Identified zones were 3-O-acetyl-β-boswellic acid (triterpenoid) from Boswellia carteri gum-resin (Burseraceae), pimpinellin and psoralen (furocoumarins) from Heracleum persicum flowers (Apiaceae), oleuropein (seco-iridoid) from Olea europaea leaves (Oleaceae), harmine, harmaline, vasicine, deoxyvasine (alkaloids) from Peganum harmala seeds (Zygophyllaceae), costic acid (sesquiterpene) from Nardostachys jatamansi hypocotyl (Valerianaceae), elaidic, linoleic, palmitic, palmitoleic acids (fatty acids) from Pistacia atlantica fruits (Anacardiaceae).

      Classification: 4e, 8b, 11a, 15a, 22, 32e
      128 048
      Cholestasis impairs hepatic lipid storage via AMPK and CREB signaling in hepatitis B virus surface protein transgenic mice
      K. IRUNGBAM, M. RODERFELD, H. GLIMM, F. HEMPEL, F. SCHNEIDER, L. HEHR, D. GLEBE, Y. CHURIN, G. MORLOCK, I. YÜCE, Elke ROEB* (*Department of Gastroenterology, Justus Liebig University Giessen, Giessen, Germany;

      Nature - Lab. Invest. 100, 1411–1424 (2020). Samples were chloroform – methanol 1:1 solutions of lipid standards and of liver tissue extracts from wild-type mice (1), from transgenic murine models of hepatic steatosis (2) (mice expressing HBs, hepatitis B virus surface protein), or of cholestasis (3) (mice totally knock-out for the gene of phospholipid translocator ABCB4, ATP-binding cassette subfamily B member 4), or of both (4) (hybrids of mice (2) and (3)). HPTLC on silica gel (preheated at 110°C for 15 min) with n-hexane – diethyl ether – acetic acid 20:5:1. (A) For qualitative analysis, visualization under white light after immersion into anisaldehyde 0.5 % (in sulfuric acid – acetic acid – methanol, 1:2:17), followed by heating at 110°C for 9 min. (B) Identification of lipids was confirmed by elution of the zones of interest with methanol from the HPTLC layer through a TLC-MS interface and a filter frit directly to a quadrupole-orbitrap MS (atmospheric pressure chemical ionization, full HR-MS scan in m/z range 100–1000). (C) For quantitative analysis, visualization at UV 366 nm after derivatization by immersion into primuline reagent (primuline 0.5 g/L in acetone – water 4:1); fluorescence was measured at UV 366 nm (mercury lamp, optical filter for wavelengths above 400 nm, scanning slit 6.0 mm × 0.2 mm, speed 20 mm/s). (A) and (B) allowed the separation and detection of cholesterol, cholesteryl oleate, methyl oleate, free fatty acids (FFA, expressed as oleic acid equivalents) and triacylglycerols (TAG, as triolein equivalents) in liver extracts. (C) showed that TAG was decreased and FFA increased in (3) and (4), compared to (1) and (2). Cholesterol and cholesteryl oleate had no significant changes between groups.

      Classification: 4e, 11a, 11c, 13c
      128 038
      Potential benefits of structured lipids in bulk compound chocolate: Insights on bioavailability and effect on serum lipids
      R. LEDESMA, R. MARTINEZ, D. CURIEL, L. FERNANDEZ, M. SILVA, A. CANALES, J. RODRIGUEZ, J. MATEOS, Ana PREZA*, M. MADRIGAL (*Research and Development Department, Alpezzi Chocolate, S.A. de C.V., Prolongaci´on Los Robles Sur, Los Robles, 45134 Zapopan, Jalisco, Mexico,

      Food Chem. 375, 131824 (2022). HPTLC of triacyclglycerols (1), diglycerides (2), monoglycerides (3) and medium and long chain free fatty acids (4) in an alternative
      functional food through bulk compound chocolate on silica gel with hexane - ethyl ether - acetic acid 80:20:1. Detection by spraying with 50 % sulfuric acid, following by heating at 150 °C for 10-15 min. Quantitative determination by absorbance measurement at 500 nm.

      Classification: 11a
      128 045
      Eight different bioactivity profiles of 40 cinnamons by multi-imaging planar chromatography hyphenated with effect–directed assays and high-resolution mass spectrometry
      N. SUMUDU, Gertrud MORLOCK* (*Justus Liebig University Giessen, Institute of Nutritional Science, Chair of Food Science, and TransMIT Center for Effect–Directed Analysis, Heinrich–Buff–Ring 26–32,
      35392 Giessen, Germany,

      Food Chem. 357, 129135 (2021). HPTLC of cinnamon on silica gel with toluene - ethyl acetate - methanol 6:5:3. Nine detection modes were used: 1) white light illumination, 2) UV 366 nm, 3) UV 254 nm, and six different derivatization reagents applied by immersion: 4) primuline reagent (100 mg primuline, 20 mL water and 80 mL acetone), 5) p-anisaldehyde sulfuric acid reagent (1 mL methoxy benzaldehyde, 140 mL methanol, 16 mL acetic acid and 8 mL sulfuric acid), 6) vanillin sulfuric acid reagent (1 g vanillin, 80 mL ethanol and 0.8 mL sulfuric acid), 7) diphenylamine aniline o-phosphoric reagent (2 % each of diphenylamine and aniline in 100 mL isopropanol plus 20 mL o-phosphoric acid), 8) Fast Blue B salt reagent (100 mg Fast Blue B salt in 100 mL ethanol, 70 %) and 9) natural product reagent (1 g 2-aminoethyl diphenyl borate in 100 mL ethanol), followed by heating at 110 °C (5), 120 °C (4, 6) or 140 °C (7, 8) for 3-5 min. Effect-directed profiling was performed through eight different assays: HPTLC–Aliivibrio fischeri bioassay, HPTLC–Bacillus subtilis bioassay, HPTLC–tyrosinase inhibition assay and densitometric evaluation, HPTLC–α–glucosidase and β–glucosidase inhibition assays, HPTLC–AChE and BChE inhibition assays, HPTLC–DPPH assay. Compounds were further characterized by heated electrospray ionization high–resolution mass spectrometry (HESI–HRMS).

      Classification: 9, 11a
      127 031
      Determination of mono- and diacylglycerols from E 471 food emulsifiers in aerosol whipping cream by high-performance thin-layer chromatography–fluorescence detection
      Claudia OELLIG*, M. BLANKART, J. HINRICHS, W. SCHWACK, M. GRANVOGL (*Department of Food Chemistry and Analytical Chemistry (170a),
      Institute of Food Chemistry, University of Hohenheim, Garbenstrasse 28, 70599 Stuttgart, Germany,

      Anal. Bioanal. Chem. 412, 7441-7451 (2020). HPTLC of mono- (1) and diacylglycerol (2) emulsifiers (E 471) in whipping creams on primuline impregnated silica gel with n-pentane - n-hexane - diethyl ether 9:9:22. Quantitative determination by fluorescence measurement at UV 366/> 400 nm. The hRF values for (1) and (2) were 10 and 52, respectively. Linearity was between 1.5 and 20 ng/zone for (1) and (2). Intermediate precision was below 7 % (n=4). The LOD and LOQ were 1.8 and 5.7 ng for (1) and (2). Recovery was between 95 and 105 % for (1) and 86 and 95 % for (2).

      Classification: 11a
      127 002
      Low temperature plasma probe mass spectrometry for analytes separated on thin-layer chromatography plates: direct vs. laser assisted desorption.
      X. GONG, D. ZHANG, I. B. EMBILE, Y. SHE, S. SHI, G. GAMEZ* (*Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas, USA;

      J Am Soc Mass Spectrom 31(9), 1981-1993 (2020). Low-temperature plasma-mass spectrometry was studied for comparison between direct desorption (DD) and diode laser assisted desorption (LD) in terms of quantitative and qualitative analysis of compounds from cellulose vs. silica gel TLC layers. Compounds (the 20 common amino acids, propofol, nicotine, cotinine, salicylamide, acetylsalicylic acid, paracetamol, caffeine, valprolactone and its isomer 4-ene-valproic acid) were applied on the TLC plates (without development) at different concentrations; a commercial mixture of acetylsalicylic acid, paracetamol and caffeine was also applied on TLC plates, developed with dichloromethane – ethyl acetate 1:50, detection at UV 254 nm and quantitative MS. In general, DD provided good results on cellulose, where LODs where between 0.01 and 2.55 ng/mm2, whereas several compounds remained undetected on silica gel. LD however provided LODs on silica gel from 0.3 to 84 pg/mm2. Tandem MS with collision-induced dissociation was implemented to improve signals, LODs and to characterize the other analytical figures-of-merits (including detection of the main fragment ions, determination of optimal laser beam width and irradiance depending on the compounds). For the two metabolites of valproic acid, the ions and fragments had identical values; therefore, a mix of the two isomers had to be applied and separated with dichloromethane – methanol 50:1 before MS; one half of the plate was visualized for control by dipping into potassium permanganate reagent (7.5g KMnO4, 50g K2CO3, 0.75g NaOH in 1L water).

      Classification: 4e, 7, 8b, 11a, 18a, 22
      127 073
      A validated stability-indicating HPTLC assay for determination of 10-hydroxy-2-decenoic acid content in royal jelly products using robust regression methods
      M.A. KORANY, M.S. MONEEB*, A.M. ASAAD, N.A. EL-SEBAKHY, A.A. EL-BANNA (*Dep. of Pharm. Anal. Chem., Fac. of Pharmacy, Univ. of Alexandria, El-Khartoum square - Azarita, Alexandria 21521, Egypt,

      J. of Chromatogr. Sci. 58 (6), 520 – 534 (2020). HPTLC of 10-hydroxy-2-decenoic acid (10-HDA) in royal jelly products marketed in Egypt, on silica gel with chloroform - acetic acid 10:1. Quantitative determination by densitometry at 210 nm. First and second derivative treatment of the data and comparison between three statistical regression methods: parametric, nonparametric and weighted regression (WR). Derivative treatment of the data improved the sensitivity of the chromatographic signals. The WR method was advantageous over the use of the other two models and resulted in an enhancement of the accuracy and precision of the 10-HDA analysis. Recovery was 99.9 % with WR and 99.6 and 98.6 % with the other statistical methods. Further, the royal jelly standard was subjected to forced degradation studies including the effect of hydrolysis, oxidation, photolysis and dry heat.


      Classification: 11a
      125 025
      Computational and experimental validation of free radical scavenging properties of high-performance thin-layer chromatography quantified phenyl myristate in Homalium nepalense
      S. KANHAR, P. ROY, A. SAHOO* (*Medicinal& Aromatic plant division, Regional Plant Resource Centre, Forest& Environment Department, Govt. of Odisha, Nayapalli, Bhubaneswar-751015, India,

      J. Sep. Sci. 43, 1566-1575 (2020). HPTLC of phenyl myristate in the bark and leaves of Homalium nepalense on silica gel with chloroform - methanol 9:1. Quantitative determination by absorbance measurement at 254 nm. The hRF value for phenyl myristate was 49. Linearity was in the range of 100-500 ng/5 µL. Intermediate precisions were below 1 % (n=5). The LOD and LOQ were 3 and 10 ng/5 µL, respectively. Average recovery was  between 90.1 and 95.6 %.

      Classification: 11a