J. Liq. Chromatogr. Relat. Technol. 32, 41-46 (2019). HPTLC of flavonoids apigenin, luteolin, chrysin, myricetin, prunin (or naringenin 7-O-glucoside), nicotiflorin (or kaempferol 3-O-rutinoside), rutin (or quercetin 3-O-rutinoside), quercetin 3-O-glucopyranoside, luteolin 7-O-glucoside, isovitexin (or apigenin-6-C-glucoside), apigenin-7-O-glucoside, naringenin, hesperetin, flavone, kaempferide, kaempferol, naringin, hesperidin, quercetin dihydrate and quercetin in Caigua (Cyclanthera pedata Scrabs) on silica gel (1) or RP-18 (2) with ethyl acetate - water - formic acid 17:3:2 for (1) or 5 % formic acid in methanol - water 7:3 for (2). Detection by heating at 110 ºC, followed by dipping into Natural product reagent for 2 min. Qualitative identification under UV light at 254 nm and 366 nm. Flavonoids were further analyzed by HPTLC–MS/(MSⁿ). 

J. Planar Chromatogr. 32, 103-108 (2019). HPTLC of ursolic acid (1), β-sitosterol (2), lupeol (3) and quercetin (4) in the aerial parts of Ichnocarpus frutescens on silica gel with toluene - ethyl acetate - formic acid 80:20:1. Quantitative determination by absorbance measurement at 500 nm for (1), 550 nm for (2), 650 nm for (3) and 310 nm for (4). The hRF values for (1) to (4) were 66, 70, 75 and 42, respectively. Linearity was between 100 and 600 ng/zone for (1) to (4). The intermediate precision was below 2 % (n=3). The LOD and LOQ were 60 and 182 ng for (1), 80 and 242 ng for (2), 50 and 151 ng for (3) and 45 and 137 ng for (4), respectivley. Recovery rate was between 96.9 and 100.1 % for (1) to (4).

J. Planar Chromatogr. 32, 121-126 (2019). HPTLC of quercetin (1), rutin (2), and
gallic acid (3) in the aerial parts of Amaranthus tricolor on silica gel with toluene - ethyl acetate - formic acid 7:5:1. Quantitative determination by absorbance measurement at 254 nm. The hRF values for (1) to (3) were 49, 14 and 28, respectively. Linearity was between 200-800 ng for (1), 200-500 ng for (2) and 200-600 ng for (3). The intermediate precision was below 1 % (n=6). The LOD and LOQ  were 22 and 68 ng/zone for (1), 14 and 55 ng/zone for (2) and 16 and 52 ng/zone for (3), respectively. Recovery was between 99.0 and 99.9 % for (1), 98.4 and 99.3 % for (2) and 99.1 and 99.8 % for (3).

J. Planar Chromatogr. 32, 31-39 (2019). HPTLC of quercetin in the roots of Phoenix sylvestris on silica gel with toluene - ethyl acetate - formic acid 5:4:1. Quantitative determination by absorbance measurement at 254 nm. The hRF value of quercetin was 39. Linearity was between 50 and 250 ng/zone. The intermediate precision was below 2 % (n=5). The LOD and LOQ were 21 and 44 ng/zone, respectively. Recovery rate was 99.4 %.

J. Planar Chromatogr. 20, 127-130 (2007). TLC of isorhamnetin-3-glucoside, isorhamnetin-3-rutinoside, kaempferol-3-rhamnosidoglucoside, isoquercitrin, rutoside, hyperoside on silica gel in horizontal chamber with ethyl acetate - methyl ethyl ketone - formic acid - water 5311 or ethyl acetate - formic acid - water 911. Quantitation by scanning at 366 nm; detection by spraying with a 1 % methanolic solution of Natural Product Reagent A followed by a 4 % methanolic solution of polyethylene glycol 400.

J. AOAC Int. 93, 804-810 (2010). HPTLC of rutin on amino phase with ethyl acetate - formic acid - methanol - water 10091117 at room temperature. Detection by spraying with natural products reagent. Linearity was between 0.95 and 4.78 µg/zone. LOD and LOQ were 330 and 630 ng/zone, respectively. The %RSD for six replicates at three concentration levels was less than 3 %, while the recovery was between 97.3-103.3 %. For the three-dimensional image analysis of chromatograms images were taken from each plate using a HP flatbed scanner at an optical resolution of 300 dpi in normal mode. The pictures were stored in TIFF file format. Evaluation was performed using Melanie 7.0 software. Following automatic detection of the zones the program computed the 3D image of a selected region from the HPTLC plate. The volume of the resulting cone-shaped zone was used as numerical data to construct the calibration curve.

Ameliorative effects of Tricholepis glaberrima in experimentally induced hepatic damage in rats modulation of cytokines functions. J. Ethnopharmacol. 160, 164-172 (2015). HPTLC of quercetin in methanolic extracts of aerial parts of Tricholepis glaberrima on silica gel (prewashed with methanol and activated at 110 °C for 10 min) with toluene - ethyl acetate - formic acid 25201. Quantitative determination by absorbance measurement at 280 nm. The hRF value of quercetin was 48.

J. Planar Chromatogr. 28, 300-306 (2015). HPTLC of ellagic acid (1), gallic acid (2) and methyl gallate (3) in gall of Quercus infectoria on silica gel with toluene - ethyl acetate - formic acid 1294. Quantitative determination by absorbance measurement at 300 nm. The hRF values for (1) to (3) were 19, 29 and 46, respectively. Linearity was in the range of 590-1181 ng/zone for (1), 691-2114 ng/zone for (2) and 693-2078 ng/zone for (3). LOD and LOQ were 15 and 51 ng/zone for (1), 243 and 812 ng/zone for (2) and 155 and 516 ng/zone for (3), respectively. The intermediate precision was 3.5 % (n=3). Recoveries ranged between 96 and 102 % for (1), 97 and 105 % for (2) and 95 and 97 % for (3).