J. Chinese Trad. Patent Med. (Zhongchengyao) 20 (2), 23-25 (1998). TLC on silica gel with chloroform - ethyl acetate - methanol 50:1:1. Detection by spraying with 0.5% phosphomolybdic acid in ethanol and heating at 110°C for 8-10 min. Quantitation of b-cholesterol by densitometry at 600 nm, and of total flavonoids by UV spectrophotometry. Precision for b-cholesterol = 2.94% (n=4). Recovery 98.64 ± 2.28% (n=4).

J. Liq. Chrom. & Rel. Technol. 22, 83-103 (1999). Determination of criteria for the evaluation of the most efficient chromatographic system. TLC of flavonoids (kaempferol, quercetin, quercitrin, hyperoside) on silica gel with 15 different solvent systems as eluents in paper-lined separation chambers, equilibrated for 30 min. Visualization by spraying with 1% methanolic diphenylboryloxyethylamine, followed by 5% ethanolic polyethylene glycol 4000.

Chromatographia 52 (5/6), 357-362 (2000). Determination of the relationship between Rf and modifier concentration to characterize the retention and selectivity of separations of 23 flavonoids, on silica gel and diol adsorbents with mixtures of ethyl acetate - methanol, on cyanopropyl adsorbents with mixtures of ethyl acetate - dichloromethane, on aminopropyl silica gel with mixtures of ethyl acetate - methanol - water, on RP-18 with mixtures of methanol - water. Discussion of the selectivity for different systems. Use of chemometric database to choose optimum chromatographic system for the separation of flavonoides, aglycones and glycosides in a single TLC experiment.

Planta med. 66, 127-133 (2000). Preparative TLC of flavonoids ((-)-epicatechin, (-)-epigallocatechin, isoquercetin, hyperin, (+)-catechin, (+)-gallocatechin, kaempferol-6'-O-acetate, isoquercetin-6'-O-acetate, apocynin A, B, cinchonain Ia, apocynin C, D, quercetin) on silica gel RP with methanol - water 1:1, 11:9. Detection under UV.

Planta med. 68, 146-151 (2002). Analytical and preparative TLC of trifolirhizin, quercetin, euchretin K, L, M, and N on silica gel using i. a. chloroform - methanol - 29 % ammonia 100:10:1, n-hexane - chloroform 20:1 and 30:1, chloroform - methanol 10:1, 20:1 and 30:1 as well as n-hexane - acetone 4:1, 7:1, 10:1 and 60:1. Detection by spraying with Dragendorff's reagent or 50 % sulfuric acid followed by heating on a plate heater.

Chromatographia 61 (5-6), 307-309 (2005). Similarities and differences between the retention characteristics of octadecyl silica gel wettable with water used in TLC and RP-18 used in HPLC have been elucidated by use of the linear relationships between log k and RM. The stationary phases compared were investigated with the same mobile phases - binary mixtures of methanol and water, acetonitrile and water, and tetrahydrofuran and water. For these adsorbents of the same type but differing in specific surface area the correlation line was shifted by log (alpha system I / alpha system II). High values of the correlation coefficients obtained over the whole range of mobile phase organic modifier concentration examined indicated that the TLC systems could be used to predict HPLC conditions for flavonoid separation.

J. Planar Chromatogr. 20, 317-320 (2007). HPTLC of mangiferin (a C-glucosyl xanthone) on silica gel with ethyl acetate - methanol - water - formic acid 20:2:2:1. Detection and quantification were performed densitometrically at 270 nm.

J. Planar Chromatogr. 26, 274-278 (2013). HPTLC of oroxylin A in Oroxylum indicum herbal formulations on silica gel with toluene - ethyl acetate - acetic acid 14:5:1. Quantitative determination by absorbance measurement at 275 nm. The hRf of oroxylin A was 80. Linearity was in the range of 250-1500 ng/zone. LOQ was 120 ng/zone. Recovery was in the range of 94.5-101.2 %. Intermediate/interday/intra-day precision was below 2 % (n=5). Comparable results were obatined with a validated HPLC method.