Biochemical Systematics and Ecology 28, 673-678 (2000). TLC of flavonoids on silica gel with chloroform - methanol 4:1.

leaves. J. Planar Chromatogr. 14, 415-422 (2001). HPTLC of rutin, hyperoside, astragalin, quercitrin, myricetin, apigenin, quercetin, acacetin, kaempferol, naringin, hesperidin, naringenin, chlorogenic acid, caffeic acid, and ferulic acid on silica gel with 1-propanol - n-hexane, ethyl acetate - n-hexane, tetrahydrofuran - n-hexane, 1,4-dioxane - n-hexane, ethyl methyl ketone - n-hexane, methanol - ethyl acetate, and methanol - toluene - ethyl acetate in different ratios as mobile phase for one-dimensional chromatography. For two-dimensional separation 20 systems of mobile phases were employed. Two optimum systems for two-dimensional separations were methanol - ethyl acetate 1:9 in the first and methanol - toluene - ethyl acetate 1:17:2 in the second dimension or ethyl acetate in the first and 2-propanol - n-hexane 3:5 in the second dimension. Detection by spraying with 2-(diphenylboryoxo)ethylamine and then with poly(ethylene glycol) 4000 and visualization under UV 254 and 366 nm.

J. Agric. Food Chem. 51, 4101-4110 (2003). 2D-TLC of glycosides and catechin/epicatechin monomers and dimers on silica gel with tert-butanol - water - acetic acid (3:1:1; first direction) and, after drying, with 6% acetic acid in water (second direction). Visualization by spraying with vanillin - hydrochloric acid reagent. Improved method.

J. Planar Chromatogr. 17, 261-263 (2004). HPTLC of aglycones and glycosides (flavone, hesperetin, naringenin, apigenin, kaempferol, quercetin, myricetin, tiliroside, apigenin 7-glucoside, myricitrin, kaempferol 3,7-dirhamnoside, hyperoside, hesperidin, rhoifolin, rutin, naringin) on silica gel by using mixtures of dichloromethane and ethyl acetate for aglycones and mixtures of ethyl acetate and formic acid for glycosides. The plates were conditioned for 15 min and developed face down in a horizontal chamber. To separate mixtures of flavonoids two-step gradient devlopment was used. In the first step (for gycosides) the plates were developed to a distance of 65 mm with ethyl acetate - formic acid - water 170:30:1. In the second step (for aglycones ) the plates were developed to a distance of 95 mm with dichloromethane - ethyl acetate - formic acid 170:30:1. Quantitation by densitometry at 254 nm.

Acta Chromatographica 20(3), 283-307 (2008). TLC of twenty flavonoids and their analogues on different stationary phases (non-polar and polar bonded stationary phases, silica gel, amino phase, diol phase) developed with a variety of binary mobile phases (aqueous and non-aqueous). Evaluation of similarities and differences among the chromatographic systems by principal component analysis and hierarchical clustering. Application of scoring indices to the separation power of a given system or a pair of systems allowed selection of the most suitable systems either to perform two-dimensional separations or to enhance the overall resolution by merging two stationary phases. On the basis of the investigation relatively efficient two-dimensional system on amino phase were developed. TLC with tetrahydrofuran – water 9:1 in the first dimension and acetonitrile – water 9:1, 4:1, 3:1, or 7:3 in the second dimension was found to be suitable for the separation of the compounds. Theoretically the compounds were best separated by combining diol and amino phases and using methanol – water 3:2 and acetonitrile – water 9:1, respectively.

Food Chem. 173, 749-754 (2015). Micro TLC of coloured materials in dried herbs as well as meads with juice mixed with 1 mM methanolic solution of DPPH on silica gel with hexane - acetone - ethanol 30:19:1. The method allowed to measure the total antioxidant potential.

J. Liq. Chromatogr. Relat. Technol. 38, 1142-1146 (2015). HPTLC-DPPH of instant grits with Chamomilla anthodium as additive prepared by extrusion method on silica gel with acetonitrile - water - choroform - formic acid 12:3:2:1. Detection by dipping into 0.2 % chloroformic DPPH solution for 5 s followed by keeping in the dark for 30 min.

Planta Medica 82 (15), 1374-1380 (2016). To monitor the subfractionation with methanol on cyclodextrin of a butanol fraction of a methanolic Soxhlet extract of Tamaris Africana shoots, TLC on silica gel with n-butanol – acetic acid – water 12:3:5 and chloroform – methanol – water 70:30:3. Chromatograms were derivatized with Natural Product/PEG reagent and with cerium sulfate sulfuric acid reagent. From the obtained subfractions, flavonoids (three sulfated) and phenolic compounds (ferulic and veratric acid derivatives) could be further isolated.