Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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      126 064
      Development and validation of an HPTLC–DPPH assay and its application to the analysis of honey
      M. ISLAM, T. SOSTARIC, L. YONG, K. HAMMER, Cornelia LOCHER* (*Cooperative Research Centre for Honey Bee Products Limited (CRC HBP), Crawley, Western Australia, Australia, connie.locher@uwa.edu.au)

      J. Planar Chromatogr. 33, 301-311 (2020). HPTLC of gallic acid in honey on silica gel with toluene - ethyl acetate - formic acid 6:5:1. Detection by derivatization with 2 mL of 0.4 % DPPH reagent. Quantitative determination under white light. The hRF value for gallic acid was 29. Linearity was between 40 and 140 ng/zone. Intermediate precision was below 4 % (n=3). The LOD and LOQ were 14 and 43 ng, respectively. Recovery was between 99.9 and 101.4 %.

      Classification: 8a
      126 012
      Development of a novel HPTLC fingerprint method for simultaneous estimation of berberine and rutin in medicinal plants and their pharmaceutical preparations followed by its application in antioxidant assay
      S. SATIJA, M. TAMBUWALA, K. PABREJA, H. BAKSHI, D. CHELLAPPAN, A. ALJABALI, S. NAMMI, T. SINGH, H. DUREJA, G. GUPTA, K. DUA, M. MEHTA*, M. GARG (*School of Pharmaceutical Sciences, Lovely Professional University, Jalandhar-Delhi G.T. Road (NH-1) Phagwara Punjab 144411 India, meenu18288@gmail.com)

      J. Planar Chromatogr. 33, 313-319 (2020). HPTLC of berberine (1) and rutin (2) on silica gel with n-hexane - ethyl acetate - glacial acetic acid - methanol 100:11:11:25. Quantitative determination by absorbance measurement at 241 nm. Antioxidant activity was determined by dipping into a 0.05 % DPPH solution followed by qualitative analysis at 366 nm. The hRF values for (1) and (2) were 67 and 47, respectively. Linearity was between 0.2 and 1.4 µg/zone for (1) and 2 and 14 µg/zone for (2). Intermediate precision was below 1 % (n=6). The LOD and LOQ were 0.2 and 2 µg for both (1) and (2), respectively. Average recovery was 94.9 % for (1) and 96.3 % for (2).

      Classification: 8a, 22
      125 020
      Extraction, isolation and identification of kaempferol 3,7 – diglucoside in the leaf extracts of Evolvulus alsinoides (Linn.) and its inhibition potency against α-amylase, α-glucosidase, acetylcholinesterase and amyloid aggregation
      P. SUNDARAMOORTHY*, K. PACKIAM (*Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Erode, Tamil Nadu, India, pavithramks@bitsathy.ac.in)

      Pharmacogn. Mag. 16, 227-234 (2020). HPTLC fingerprint of Evolvulus alsinoides on silica gel with toluene - ethyl acetate - formic acid 14:6:1. Qualitative determination under UV light at 254 nm. Detection of antioxidant molecules using 2,2‑Diphenyl‑1‑picrylhydrazyl (DPPH) assay.

      Classification: 8a
      125 004
      Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species
      D. ZAGORAC*, M. AKSIC, V. GLAVNIK, U. GASIC, I. VOVK, Z. TESIC, M. NATIC (*Innovation centre of the Faculty of Chemistry, University of Belgrade, Studentski trg 12–16, 11000 Belgrade, Serbia, ddabic@chem.bg.ac.rs)

      J. Sep. Sci. 43, 1431-1439 (2020). HPTLC fingerprint of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) on silica gel with n-propanol - water - acetic acid 4:2:1. Detection by dipping into 4-dimethylaminocinnamaldehyde solution (60 mg  in 13 mL of concentrated hydrochloric acid, which was made up to 200 mL with ethanol). Qualitative determination under UV light at 366 nm. The blue zones visible on the derivatized plate were used for further analysis using a TLC-MS interface. The flavanol and proanthocyanidin profiles of Rosa species depend on the geographical origin rather than on the cultivar and genotype.

      Classification: 8a
      125 032
      Effect directed detection of Rhodiola rosea L. root and rhizome extract
      H. NIKOLAICHUK, M. STUDZINSKI, Irena CHOMA* (*Department of Chromatography, Faculty of Chemistry, Institute of Chemical Sciences, Maria Curie Sklodowska University, Lublin, Poland, irena.choma@poczta.umcs.lublin.pl)

      J. Liq. Chromatogr. Relat. Technol. 43, 361-366 (2020). HPTLC of the dried root and rhizome of Rhodiola rosea on silica gel with ethyl acetate - methanol - water 77:13:10. Detection by spraying with 1) a solution of p-anisaldehyde (0.5 mL in 85 mL methanol, 10 mL acetic acid and 5 mL sulfuric acid), followed by heating at 105 ºC for 5-7 min, 2) a solution of 2-isopropyl-5-methylphenol (0.5 g in 95 mL ethanol and 5 mL sulfuric acid), followed by heating at 120 ºC and 3) NP solution (1 g diphenylboryloxyethylamine in 100 mL methanol) and PEG solution (5 g PEG-4000 in 100 mL ethanol). Detection under UV 254 and 366 nm. Effect directed detection was performed using 1) DPPH* radical reagent assay: spraying with 0.2 % 2,2-diphenyl-1-picrylhydrazyl solution in methanol, 2) AChE assay: spraying with the enzyme solution (20 units of AChE and 150 mg BSA in 150 mL 0.05 M TRIS buffer, pH 7.8), follwed by incubation at 37 ºC for 20 min and spraying with 50 mg Fast Blue B salt diluted in 100 mL of water and 3) Bacillus subtilis bioassay: dipping into bacterial suspension for 8 s, followed by incubation at 37 ºC for 17 h and spraying with 0.2 % MTT aqueous solution. The bioautographic tests showed presence of both antioxidants (DPPH assay) and antibacterials (Bacillus subtilis assay) in the methanolic plant extract, however no acetylcholinesterase inhibitors were found. As marker compound, rosavin was detected.

      Classification: 6, 8a
      125 011
      New phenylethanoid glycosides from Cistanche phelypaea and their activity as inhibitors of monoacylglycerol lipase (MAGL)
      K. AYA BELADJILA, D. BERREHAL, N. DE TOMMASI, C. GRANCHI, G. BONONI, Alessandra BRACA*, M. DE LEO (*Department of Pharmacy, University of Pisa, Pisa, Italy; alessandra.braca@unipi.it)

      Planta Medica 84(9/10), 710-715 (2018). The fractionation of an n-butanol extract of Cistanche phelypaea (Orobanchaceae) aerial parts through cyclodextran column chromatography with methanol was monitored on TLC silica gel with chloroform – methanol – water 70:30:3, detection by spraying with cerium sulfate reagent. In the 11 major fractions obtained, 4 new phenylethanoid glycosides were further identified, as well as brandioside (a phenylpropanoid glycoside), and heterosides of apigenin (flavonoid) and of pinoresinol (lignan).

      Classification: 7, 8a, 32e
      125 029
      High-performance thin-layer chromatographic investigation of rutin in the leaves of Phoenix sylvestris in sequence with pharmacognostical and phytochemical evaluation
      P. JAIN*, S. JAIN, P. CHAK, S. SWAMKAR, S. SHARMA, S. PALIWAL (*Department of Pharmacy, Banasthali Vidyapith, Banasthali 304022, India, pankaj.jain.manipal@gmail.com)

      J. Planar Chromatogr. 33, 191-201 (2020). HPTLC of rutin in the leaves of Phoenix sylvestris on silica gel with chloroform - methanol - formic acid 10:40:1. Quantitative determination by absorbance measurement at 265 nm. The hRF value for rutin was 71. Linearity was between 200 and 1000 ng/zone. Intermediate precisions were below 2 % (n=5). The LOD and LOQ were 125 and 205 ng/zone. Average recovery was found to be in the range of 99.0-100.0 %.

       

      Classification: 8a
      125 052
      A new thin-layer chromatography–direct bioautography assay for the qualitative and quantitative determination of peroxidase inhibitors in plant extracts
      R. DARWISH, E. SHAWKY, H. HAMMODA, F. HARRAZ (*Department of Pharmacognosy, Faculty of Pharmacy, Alkhartoom square, Alexandria University, Alexandria 21521, Egypt, eman.m.shawky@alexu.edu.eg)

      J. Planar Chromatogr. 33, 79-87 (2020). HPTLC of quercetin in the aerial parts of Juniperus communis, J. horizontalis and J. chinensis on silica gel with ethyl acetate - methanol - water - acetic acid 900:80:40:7. Assessment of peroxidase inhibition activity by dipping into peroxidase enzyme solution (10 mg/mL solution of peroxidase in cold phosphate buffer (pH 7), followed by dilution with 50 mL of phosphate buffer, pH 7) for 3 s, followed by heating at 35 ºC for 2 min, dipping into a hydrogen peroxide solution and finally dipping into a benzidine solution (4 g in 700 mL methanol). Linearity was between 0.85 and 9 µg/zone for quercetin. Intermediate precisions were below 3 % (n=3). The LOD and LOQ were 280 and 850 ng/zone. Recovery was between 96.6 and 99.7 %.

       

      Classification: 8a