Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      128 093
      High-throughput enzyme inhibition screening of 44 Iranian medicinal plants via piezoelectric spraying of planar cholinesterase assays
      E. AZADNIYA, I. THOMÄ, J. BAAKE, Gertrud E. MORLOCK* (*Institute of Nutritional Science, and TransMIT Center for Effect-Directed Analysis, Justus Liebig University Giessen, Giessen, Germany; gertrud.morlock@uni-giessen.de)

      Journal of Chromatography B, 1184, 122956 (2021). Test for acetyl- and butyrylcholinesterase (AChE and BChE) inhibition without development of piperin (standard inhibitor of AChE and BChE) and ethanol – water (3:2) extracts of Iranian plants, on HPTLC silica gel prewashed twice with methanol – water 3:2 and dried 60 min at 120°C. After sample application the plate was immersed (speed 3.5 cm/s, time 2 s) into enzyme solution (6.6 units/mL AChE or 3.3 units/mL BChE in TRIS buffer 0.05 M, with bovine serum albumin 0.1 %, pH 7.8), incubation 25 min at 37°C and immersion (speed 3.5 cm/s, time 1 s) into chromogenic substrate solution (α-naphthyl acetate 0.1 % and Fast Blue salt B 0.2 % in ethanol – water, 1:2). Seven mobile phases were tested for the active samples. Best separation was obtained with toluene – ethyl acetate – formic acid – water 4:16:3:2 and with toluene – ethyl acetate – methanol 6:3:1. Before enzymatic assay, plates developed with acidic mobile phases were neutralized by spraying 3 mL citrate phosphate buffer (Na2HPO4 8 %, citric acid q.s. ad pH 7.5) followed by 10 min of automatic drying. Enzymatic assay was performed using a piezoelectric spraying device: a) pre-wetting by spraying 1 mL TRIS buffer (0.05 M, pH 7.8); b) spraying 3 mL of the enzyme solution; c) incubation 25 min in a humid box at 37°C; d) spraying 0.5 mL substrate solution; e) 5 min drying at room temperature, and then 10 min of automatic drying. By spraying, zone shift and zone diffusion, which occurred with plate immersion, were avoided. For development control, derivatization was done by piezoelectrically spraying 4 mL of sulfuric anisaldehyde reagent (anisaldehyde – sulfuric acid – acetic acid – methanol, 1:10:20:170), followed by heating 3 min at 110°C. For identification of zones of interest, direct elution with methanol from underivatized HPTLC plates through a TLC-MS interface directly to a MS. Identified zones were 3-O-acetyl-β-boswellic acid (triterpenoid) from Boswellia carteri gum-resin (Burseraceae), pimpinellin and psoralen (furocoumarins) from Heracleum persicum flowers (Apiaceae), oleuropein (seco-iridoid) from Olea europaea leaves (Oleaceae), harmine, harmaline, vasicine, deoxyvasine (alkaloids) from Peganum harmala seeds (Zygophyllaceae), costic acid (sesquiterpene) from Nardostachys jatamansi hypocotyl (Valerianaceae), elaidic, linoleic, palmitic, palmitoleic acids (fatty acids) from Pistacia atlantica fruits (Anacardiaceae).

      Classification: 4e, 8b, 11a, 15a, 22, 32e
      128 061
      Comparative qualitative analysis of different classes of compounds in selected Australian and Indian Eucalyptus and Corymbia species: a convenient de‑replication method for the eucalypts
      I. SARAF, K. MARSH, V. KUMAR, W. FOLEY, I. SINGH* (*Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sector‑67, Sahibzada Ajit Singh Nagar, Punjab 160062, India, ipsingh67@yahoo.com)

      J. Planar Chromatogr. 34, 377-401 (2021). HPTLC of flavonoid glycosides, phloroglucinol glycosides, monoterpene glycosides and monoterpene sugar esters, triterpenoids, phloroglucinols, monomeric phloroglucinols, dimeric phloroglucinols and phloroglucinol-terpene adducts in 15 eucalypts (13 Eucalyptus and 2 Corymbia) on silica gel with multiple mobile phases. Detection by spraying with anisaldehyde-sulfuric acid reagent. Zones were scanned from 200 to 700 nm.

      Classification: 8a, 15a
      128 072
      Evaluation of the purification process of Croton tiglium L. seeds by chromatographic methods
      M. DEEPAK*, P. DIVYA, C. SULAIMAN, B. INDIRA (*Centre for Medicinal Plants Research, Arya Vaidya Sala Kottakkal, Malappuram, Kerala, India, deepakdnivas@gmail.com)

      J. Planar Chromatogr. 34, 345-351 (2021). HPTLC of Croton tiglium extracts on silica gel with toluene - ethyl acetate 9:1, toluene - ethyl acetate - methanol 6:4:1, toluene - ethyl acetate 3:2, toluene - ethyl acetate - methanol 4:6:1. Detection by spraying with anisaldehyde - sulfuric acid reagent. Identification of steroids and terpenoids on silica gel with toluene - ethyl acetate 9:1 and detection by spraying with the Liebermann‒Burchard reagent, followed by heating at 100 °C. Acidic compounds were analyzed on silica gel with toluene - ethyl acetate 9:1, followed by spraying with bromocresol green reagent. Coumarins were analyzed on silica gel with toluene - ethyl acetate - methanol 4:6:1, followed by spraying with alcoholic potassium hydroxide reagent and visualization under UV light at 366 nm.

      Classification: 8a, 15a
      128 010
      Isolation, identification, and quantification of pentylcurcumene from Geophila repens: A new class of cholinesterase inhibitor for Alzheimer’s disease
      U. DASH, S. KANHAR, A. DIXIT, J. DANDAPAT, A. SAHOO (*Medicinal & Aromatic Plant Division, Regional Plant Resource Centre, Forest & Environment Department, Govt. of Odisha, Nayapalli, Bhubaneswar 751015, India, atish_kumar1976@yahoo.co.in)

      Bioorg. Chem. 88, 102947 (2019). HPTLC of pentylcurcumene in the aerial part of Geophila repens on silica gel with benzene - methanol 3:1. Quantitative determination by absorbance measurement at 254 nm. The hRF value of pentylcurcumene was 50. Linearity was between 100 and 500 ng/zone. The intermediate precision was below 2 % (n=3). The LOD and LOQ were 16 and 49 ng/zone. Recovery was between 97.2 and 98.7 %.

      Classification: 15a
      127 003
      Lanostane triterpenes from Gloeophyllum odoratum and their anti-influenza effects
      Ulrike GRIENKE*, J. ZWIRCHMAYR, U. PEINTNER, E. URBAN, M. ZEHL, M. SCHMIDTKE, J. M. ROLLINGER (*Department of Pharmacognosy, Faculty of Life Sciences, University of Vienna, Vienna, Austria; ulrike.grienke@univie.ac.at)

      Planta Med. 85(3), 195-202 (2019). The dichloromethane fraction of an ethanolic extract from Gloeophyllum odoratum sporocarp (Gloeophyllaceae, Basidiomycetes) was submitted to a multistep purification process (conventional, flash and supercritical fluid column chromatography). At each step, fractions were monitored on TLC silica gel with dichloromethane – methanol – water 40:4:1. Detection under white and UV light after derivatization with vanillin sulfuric acid 5 % in methanol and heating. Eight triterpenes were isolated for further identification: eburicodiol, gloeophyllins B and K, hydroxylanosterol, trametenolic acid B (all five from the lanostane type), gloeophyllins A and L (C‑nor-D-homoergosteroid type), and ergosterol peroxide (ergostane type).

      Classification: 13c, 15a, 32e
      127 007
      Carotenoid composition and antioxidant potential of Eucheuma denticulatum, Sargassum polycystum and Caulerpa lentillifera.
      V. BALASUBRAMANIAM*, L. JUNE CHELYN, S. VIMALA, M.N. MOHD FAIRULNIZAL, I.A. BROWNLEE, I. AMINE (*Nutrition, Metabolism & Cardiovascular Research Centre, Institute for Medical Research, Ministry of Health Malaysia, Shah Alam, Selangor, Malaysia; vimala.rmt@moh.gov.my)

      Heliyon 6(8), e04654 (2020). HPTLC of ethanolic extracts of three algae (100µg/band) on silica gel, along with carotenoid standards (10µg/band), developed with toluene – acetone 7:3. Detection under white light. Carotenoids appeared orange or yellow, chlorophylls green, pheophytins dark khaki. Carotenoid patterns of the algae were very different depending on the family: red alga Eucheuma denticulatum (Solieriaceae) contained mainly zeaxanthin and lutein (hRF 44) and β-carotene (hRF 88), but also β-cryptoxanthin (hRF 69-71) and fucoxanthin (hRF 39); brown alga Sargassum polycystum (Sargassaceae) contained mainly fucoxanthin, and also cryptoxanthin; green alga Caulerpa lentillifera (Caulerpaceae) contained mainly zeaxanthin, but also astaxanthin (hRF 61) and canthaxanthin (hRF 77) in smaller amounts. Separately, HPLC-MS was used to confirm and quantify these compounds, which was necessary for carotenoids with similar hRF values: zeaxanthin and lutein (hRF 44), and β-carotene and lycopene (hRF 88).

      Classification: 11c, 15a, 23a, 32e
      126 020
      New hirsutinolide-type sesquiterpenoids from Vernonia cinerea inhibit nitric oxide production in LPS-stimulated RAW264.7 cells
      Li-Ming YANG KUO, Pei-Yi TSENG, Yu-Chi LIN, Chia-Ching LIAW, Li-Jie ZHANG, Keng-Chang TSAI, Zhi-Hu LIN, Hsiu-O HO*, Yao-Haur KUO (*School of Pharmacy, Taipei Medical University, Taipei, Taiwan; hsiuoho@tmu.edu.tw)

      Planta Med. 84(18), 1348-1354 (2018). A subfraction (obtained through liquid-liquid partition and column chromatography) of the ethanolic extract of whole Vernonia cinerea plants (Asteraceae, subf. Cichorioideae) was further fractioned by reverse-phase SPE (solid-phase extraction) followed by preparative TLC on silica gel layer (eluent not given). For verification, zones were detected by spraying with anisaldehyde solution with 10 % sulfuric acid, followed by heating at 100 °C. Further purification by reverse-phase HPLC allowed the isolation of 6 hirsutinolide-type sesquiterpenoids (all with a oxacyclonane forming an ether bridge), including vernolides A and B.

      Classification: 8b, 9, 15a, 32e
      126 019
      Anti-inflammatory four new 2-(2-phenylethyl)-4H-chromen-4-one derivatives from the resinous wood of Aquilaria sinensis and their inhibitory activities on neutrophil pro-inflammatory responses
      S. WANG (Wang Sin-Ling), H. LIAO (Liao Hsiang-Ruei), M. CHENG (Cheng Ming-Jen), C. SHU (Shu Chih-Wen), C. CHEN (Chen Chun-Lin), M. CHUNG (Chung Mei-Ing)*, J. CHEN (Chen Jih-Jung) (*School of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan; meinch@kmu.edu.tw)

      Planta Med. 84(18), 1340-1347 (2018). Preparative TLC on silica gel for the final purification of 15 phenylethyl-chromenone derivatives (including 3 with epoxide functions), 4 sesquiterpenoids (neopetasane, dehydrokaranone, dioxoselinene, ligudicin C) and a steroid (ergostatetraenone), all isolated through multi-step column chromatography from the ethyl-acetate fraction of a methanolic extract of Aquilaria sinensis resinous wood (Thymelaeaceae). For each compound, mobile phase, RF value and yield are given.

      Classification: 8b, 9, 15a, 32e