Cumulative CAMAG Bibliography Service CCBS
Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.
The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:
- Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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J. Liq. Chromatogr. Relat. Technol. 43, 344-350 (2020). HPTLC of hydrodistilled Plectranthus amboinicus essential oil on silica gel with n-hexane - ethyl acetate - ethanol 95:3:2. Detection by dipping into anisaldehyde sulfuric acid reagent, followed by heating at 100 ºC for 5 min. HPTLC-bioprofiling was performed using the following assays by dipping the chromatogram into the respective solution, followed by drying, incubation and documentation at white light or measuring bioluminescence: DPPH* radical reagent assay (using a 0.2 mg/mL 2,2-diphenyl-1-picrylhydrazyl solution in methanol), AChE inhibitory assay, tyrosinase inhibitory assay, alpha- and beta-glucosidase inhibitory assays, alpha-amylase inhibitory assay, Gram-negative antimicrobial bioassay (chromatogram immersion into a A. fischeri suspension), and Gram-positive antimicrobial bioassay (chromatogram immersion into a B. subtilis bacterial suspension). Direct analysis in real time mass spectrometry allowed the detection of five bioactive compounds: caryophyllene oxide (hRF 20), a-humulene (hRF 26), carvacrol (hRF 40), methyl carvacrol ether (hRF 76) and caryophyllene (hRF 84).
Planta Medica 84(9/10), 716-720 (2018). The fractionations of n-hexane and chloroform extracts of Guarea guidonia aerial parts (Meliaceae) through silica gel column chromatography was monitored on TLC silica gel with cerium sulfate / sulfuric acid as derivatization reagent. In the fractions obtained, 3 new tirucallane-type triterpenoids (guareolide, guareoic acids A and B) were further identified, as well as other terpenoids (flindissone, acetyldihydronomilin, picroquassin E, boscartol C, and acneorubins A, B, and X).
Planta Medica 84(9/10), 729-735 (2018). The chloroform fraction of a methanolic extract of Euphorbia taurinensis whole plant (Euphorbiaceae) was submitted to a multi-step fractionation through column chromatography. Monitoring by TLC on silica gel (mobile phases see below) followed by derivatization with concentrated sulfuric acid and heating at 105°C. The fractions obtained were purified by repeated cycles of preparative TLC alone or alternating with preparative HPLC, leading to the isolation of segetane, ingenane, and jatrophane diterpenes. Depending on the subfractions, preparative TLC silica gel and reverse-phase C18 layers were used, with cyclohexane – ethyl acetate – ethanol 25:15:1 for normal phase, and with mixtures of acetonitrile (or methanol) and water for RP.
Planta Medica 84(1), 59-64 (2018). A multi-step fractionation through silica gel column chromatography (CC) of a methanolic extract of Plectranthus africanus (whole plant, Lamiaceae) was monitored through TLC on silica gel with various solvent mixtures (n-hexane or dichloromethane with either acetone or methanol). Zones were detected under UV and further by spraying with sulfuric acid 20 % and heating at 100°C. For each fraction or TLC profile, the authors provide the CC gradient, the optimal proportions of the solvents used for the TLC mobile phase, as well as the RF values of the molecules isolated by this CC method: new abietane-type diterpenoids (plectranthroyleanones A, B, C), betulinic and oleanolic acids, heterosides of apigenin, rhamnetin and sitosterol.
J. Planar Chromatogr. 32, 359-364 (2019). HPTLC of Schizonepeta annua on silica gel with chloroform - toluene - acetic acid 600:100:3. Detection by spraying with 5 % vanillin - sulfuric acid reagent (mixture of 2.5 g vanillin, 45 mL ethanol, and 5 mL concentrated sulfuric acid), followed by heating at 105 °C for 5 min. Antibacterial assay was performed by dipping the plate into a cell suspension culture (Brain heart infusion broth and brain heart infusion agar in the ratio of 9:1), followed by incubation at 35 °C for 16 h and immersion into 0.2 % MTT solution for 3s, followed by incubation at 35 °C for 3h. Plates were scanned at 546 nm. The hRF values for thymol and carvacol were 88 and 78, respectively.
Planta Med. 83(16), 1289-1296 (2017). A cycloartene-diol, a dihydroxycicloartenone and an isopimaradiene-diol were isolated from subfractions of an ethanolic extract of Guarea macrophylla (Meliaceae) leaves through elution on preparative TLC silica gel layers with chloroform – acetone 19:1, or with n-hexane – ethyl acetate 9:1 or 1:1, respectively.
CBS 97, 6-7 (2006). HPTLC of absinthe (a beverage of the wormwood plant, Artemisia absinthium) on silica gel with acetone - acetic acid (98 %) - toluene - dichloromethane 1:1:3:5 over 70 mm. Detection by dipping in a solution of acetic anhydride - sulphuric acid - ethanol 1:1:10 followed by heating at 104 °C for 5 min. Quantitative determination by absorbance measurement at 554 nm. The hRf value of absinthin was 64 and selectivity regarding matrix was given. Linearity was between 0.1 and 10 g/L. The limit of detection and quantification for absinthin was 0.05 and 0.11 g/L, respectively. The precisions were better than 13.5 % (intraday) and 15.8 % (interday).
Analytical Chemistry - An Indian Journal 8(1), 77-81 (2009). An HPTLC method is reported for estimation of lupeol and beta-sitosterol in the whole plant powder of Asteracantha longifolia (Acanthaceae). The proposed method was also employed for estimation of beta-sitosterol in herbal formulation containing Asteracantha longifolia. Methanolic extracts of the sample were used for chromatography. HPTLC on silica gel with toluene - ethyl acetate - methanol 75:15:7. Derivatization with Liebermann-Burchard reagent. Densitometric quantification at 366 nm. The concentration of lupeol and beta-sitosterol was 0.162 mg/g and 0.045 mg/g, respectively in the whole plant, while the concentration of beta-sitosterol was 0.039-0.048 mg/g in the formulation. The method was suitable for the quality control of the herbal formulation.