Biochemical Systematics and Ecology 24, 37-42 (1996). TLC of sesquiterpenes on silica with butanol - acetone - water 3:1:1.

Phytochemistry 51, 429-433 (1999). TLC of new acetogenin derivatives on silica with chloroform - methanol 10:1, and ethyl acetate - acetone 15:1. Detection with Kedde's reagent. Isolation of closely related acetogenins by preparative TLC with the same solvent systems.

Juss. J. Planar Chromatogr. 16, 311-314 (2003). HPTLC of azadirachtin on silica gel with toluene - ethyl acetate - formic acid 10:8:1 with chamber saturation for 20 min. at 25°C. After drying of the plate visualization by spraying with vanillin-sulfuric acid reagent (3% vanillin in 1% ethanolic sulfuric acid) and heating at 100°C for 2 min. Quantitation by densitometry at 677 nm. Development of a simple, precise, and specific method.

Planta med. 70, 81-84 (2004). Preparative TLC of pubescene D (3ß-,9a-diacetoxy-7ß-benzoyloxy-15ß-hydroxy-14-oxo-2ßH-jatropha-5E,12E-diene) on silica gel with chloroform - acetone 9:1. Detection under UV light at 254 nm.

Pharmazie 61, 70-73 (2006). TLC of olean-12-en-3beta,11a,16beta-triol-3-O-palmitate, ursa-12-en-3beta,11beta,16beta-triol-3-O-palmitate, olean-12-en3beta,16beta-diol-3-O-palmitate, beta-amyrin, alpha-amyrin, methylursolate, taraxasterol, taraxast-20(30)-ene-3beta,16beta-diol-3-O-palmitate, pseudotaraxasterol, lup-3beta-O-palmitate on silica gel. Detection UV light or by spraying with 98 % sulfuric acid - ethanol 1:19 followed by heating at 110 °C.

J. Planar Chromatogr. 22, 49-53 (2009). TLC of geranylgeraniol and plaunotol on silica gel with chloroform - n-propanol 24:1 or 48:1, or with ethyl acetate, in saturated chambers. Quantitative determination by absorbance measurement at 210 nm. Detection of plaunotol by exposure to iodine vapor for 10 min. The acyclic diterpenoid plaunotol present in Croton stellatopilosus leaves is a hydroxylation product, catalyzed by the enzyme geranylgeraniol-18-hydroxylase. The activity of the enzyme in cell-free extracts of C. stellatopilosus leaves was previously reported. In this study a new mobile phase (ethyl acetate) was used for determination of geranylgeraniol-18-hydroxylase in the 20,000 g and 100,000 g precipitates of the crude extracts. In addition ethyl acetate successfully separated plaunotol from various cytochrome P-450 inhibitors (ancymidol, metyrapone, and miconazole) frequently used for biochemical characterization of the hydroxylase enzymes.

J. Planar Chromatogr. 26, 260-266 (2013). HPTLC of of iridoids 10-O-trans-p-coumaroylcatalpol (1), 4-hydroxy-E-globularinin (2) and premnosidic acid (3) in the stem bark of Premna integrifolia on silica gel with ethyl acetate - methanol - water - acetic acid 40:6:3:1. Detection by dipping into vanillin - sulphuric acid reagent (1 % vanillin in ethanol - sulfuric acid 19:1), followed by heating at 110 ºC for 3 min. Quantification by absorbance measurement at 510 nm. The hRf values for (1) to (3) were 52, 41 and 33, respectively. Linearity was in the range of 1-10 µg/zone for (1) to (3). LOD and LOQ were 198 and 663 ng/zone for (1), 312 and 1040 ng/zone for (2) and 200 and 666 ng/zone for (3), respectively. Average recoveries for (1) to (3) were found to be 97.3 %, 98.3 % and 97.6 %, respectively. Intermediate/interday/intra-day precision was below 2 % (n=9).

Phytochemistry 24, 1587-1591 (1985). TLC of cucurbitacins on silica with chloroform - methanol 9:1. Detection with vanillin - phosphoric acid reagent. For the detection of alpha-ketolcucurbitacins, the plates were sprayed with a 1:1 mixture of triphenyltetrazolium chloride (4 % in ethanol) and NaOH 1 N. Flavonoid glycosides were separated on silica with ethyl acetate - formic acid - acetic acid - water 100:11:11:27. Detection with diphenylboryloxyethylamine reagent, followed by PEG 4000 (5 % in ethanol, UV 365 nm.