Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. The saved items can be printed to PDF using the print function of your web browser.

      124 042
      Quantitative TLC-SERS detection of histamine in seafood with support vector machine analysis
      A. TAN (Tan Ailing), Y. ZHAO (Zhao Yong), K. SIVASHANMUGAN, K. SQUIRE, A. WANG* (*School of Electrical Engineering and Computer Science, Oregon State University, Corvallis, OR, 97331, USA, wang@engr.orst.edu)

      Food Control. 103, 111-118 (2019). Thin layer chromatography in tandem with surface-enhanced Raman scattering (TLC-SERS) of histamine in tuna samples on diatomaceous earth plates with ethanol - ammonia 3:1. Detection by spraying with Pauly's reagent (equal mixture of 20 mM sulfanilic acid in a 1 M HCl solution and 200 mM sodium nitrite solution, followed by adding 10 % anhydrous sodium carbonate in a 5 % ethanol solution). Gold nanoparticles were deposited on the plate zone and measurements were performed using a Raman spectrometer with an excitation laser wavelength of 785 nm.

      Classification: 4e, 17a
      124 055
      Direct bioautography hyphenated to direct analysis in real time mass spectrometry: Chromatographic separation, bioassay and mass spectra, all in the same sample run
      T.T. HÄBE, M. JAMSHIDI-AIDJI, J. MACHO, Gertrud E. MORLOCK* (*Chair of Food Science, Institute of Nutritional Science, Interdisciplinary Research Center (IFZ), Justus Liebig Univ. Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany, Gertrud.Morlock@uni-giessen.de)

      J. of Chromatogr. A 1568, 188-196 (2018). Mass spectra by DART-MS were recorded directly in situ the bioautogram, immediately after direct bioautography (DB). This allowed to detect bioactive analytes within the bioautogram and discriminate microorganism cells and polar bioassay medium ingredients which could otherwise stress the MS system. DB-DART-MS was used for bioactive compounds in cosmetics using the Bacillus subtilis and Aliivibrio fischeri bioassays for detection of Gram-positive and Gram-negative antimicrobials. Planar yeast estrogen screen was used for detection of estrogen-effective compounds. HPTLC-DART-MS of parabens in hand creams either on silica gel with petroleum ether - glacial acetic acid 20:3 or on RP-18W with methanol - water 1:1. Detection under UV 254 and 366 nm. Bioassay by immersing the neutralized chromatograms into the bacterial suspensions.

      Keywords: densitometry HPTLC
      Classification: 4e, 5b
      124 062
      Rapid isolation of a dipeptidyl peptidase IV inhibitor from Fritillaria cirrhosa by thin-layer chromatography–bioautography and mass spectrometry-directed autopurification system
      L. GU, T. TIAN, L. XIA, G. CHOU*, Z. WANG (*Key Laboratory of Standardization of Chinese Medicines, Ministry of Education, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China, chouguixinzyb@126.com)

      J. Planar Chromatogr. 32, 447-451 (2019). HPTLC of Fritillaria cirrhosa on silica gel with ethyl acetate - methanol - ammonia solution - water 180:20:10:1. Bioautography by dipping into a 0.15 mg/mL solution of substrate Gly-Pro-p-nitroanilide hydrochloride in 50 % of ethanol, followed by ethanol removal in the hood and dipping into a 10 U/L DPP IV enzyme solution in TrisHCl buffer (pH 8.2, 70 mM), followed by incubation at 37°C for 40 min. Detection by dipping into a solution of 0.5 % sodium nitrite in 1.2 M hydrochloric acid, followed by drying slightly for 5 min and dipping into 0.05 % N-(1-naphthyl)ethylenediamine dihydrochloride solution. Further analysis by mass spectrometry using a TLC interface. The hRF value for the dipeptidyl peptidase IV inhibitor was 58.

      Classification: 4e, 8b
      123 050
      Direct bioautography hyphenated to direct analysis in real time mass spectrometry: Chromatographic separation, bioassay and mass spectra, all in the same sample run
      T. T. HÄBE, Maryam JAMSHIDI-AIDJI, Jennifer MACHO, Gertrud E. MORLOCK* (*Chair of Food Sci., Inst. of Nutrit. Sci., and Interdiscipl. Res. Center (iFZ), Justus Liebig Univ. Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany, Gertrud.Morlock@uni-giessen.de)

      J. Chromatogr. A 1568, 188-196 (2018). Application of an advantageous combination, the desorption-based direct analysis in real time mass spectrometry (DART-MS) immediately after direct bioautography (DB), i.e., in the presence of microorganisms, bioassay medium and substrate reagent. The method offers a straightforward and efficient mass spectrometric detection of bioactive analytes within the bioautogram. It discriminated microorganism cells and highly polar bioassay medium ingredients which could otherwise stress the MS system. Investigation of DB-DART-MS for bioactive compounds in cosmetics using the Bacillus subtilis and Aliivibrio fischeri bioassays for detection of Gram-positive and Gram-negative antimicrobials, respectively, and the planar yeast estrogen screen for detection of estrogen-effective compounds. Study of the influences of three different bioassay matrices on the analyte response and DB-DART-MS performance on different layers (NP and RP) on the example of parabens in hand creams. Ion suppression was enhanced with increasing culture medium complexity. The mass spectrometric quantification by DB-DART-MS at the ng-level in situ each different bioautogram was verified by comparison to HPTLC-DART-MS. The total paraben content of hand creams 1 and 2 was 0.17–0.20% and 0.30–0.34%, respectively, depending on the method used. It proved that DB-DART-MS is a reliable qantitative bioanalytical hyphenation.

      Keywords: cosmetics HPTLC
      Classification: 4e
      123 007
      Effect-directed profiling of aqueous, fermented plant preparations via high-performance thin-layer chromatography combined with in situ assays and high-resolution mass spectrometry
      Maryam JAMSHIDI-AIDJI, Jennifer MACHO, Margit MUELLER, Gertrud MORLOCK* (*Institute of Nutritional Science, Interdisciplinary Research Center (IFZ), Justus Liebig University Giessen, Heinrich-Buff-Ring 26–32, 35392 Giessen, Germany, Gertrud.Morlock@uni-giessen.de )

      J. Liq. Chromatogr. Relat. Technol. 42, 266-273 (2019). HPTLC of aqueous, fermented plant preparations from Chamomilla recutita L. (1), Allium cepa L. (2), Equisetum arvense L. (3) and Hamamelis virginiana L. (4) of different harvest years on silica gel with ethyl acetate - toluene - formic acid - water 16:4:3:2. The method was combined with effect-directed analysis (EDA) and high-resolution mass spectrometry (HRMS). For α-/β-glucosidase assays, the plate was sprayed with 2 mL substrate solution (60 mg 2-naphthyl-α-D-glucopyranoside or 2-naphthyl-β-D-glucopyranoside in 50 mL ethanol), then sprayed with 1 mL sodium acetate buffer and 2 mL enzyme solution (500 units α-glucosidase), followed by incubation at 37 ºC for 10 min. Analysis of multi-potent compounds was also performed using the 2,2-diphenyl-1-picrylhydrazyl reagent and Gram-positive Bacillus subtilis assays, followed by recording of elution head-based HPTLC-ESI-HRMS spectra. 

       

      Classification: 4e
      123 010
      Diterpene lipo-alkaloids with selective activities on cardiac K+ channels
      T. KISS, B. BORCSA, P. ORVOS, L. TÁLOSI, J. HOHMANN, D. CSUPOR* (*Department of Pharmacognosy, Faculty of Pharmacy, University of Szeged, Szeged, Hungary; csupor.dezso@pharmacognosy.hu)

      Planta Med. 83(17), 1321-1328 (2017). Benzoyl-aconine esters (lipo-alkaloids) produced by transesterification of aconitine (isolated from Aconitum sp.) with long-chain fatty acids were purified by a multistep chromatographic method, including cyclodextrane gel filtration chromatography, centrifugal planar chromatography on aluminium oxide layer using cyclohexane – chloroform – methanol 70:30:1 followed by 70:30:3 and/or preparative thin-layer chromatography on aluminium oxide layer with toluene – acetone – ethanol – concentrated ammonia 70:40:10:3.

      Classification: 4d, 4e, 11a, 22, 32e
      71 057
      Isolation and identification via high performance liquid chromatography and thin-layer chromatography of benzoxazolinone precursors from Consolida orientalis flowers
      S. ÖZDEN*, T. ÖZDEN, I. ATTILA, M. KÜCÜKTSLAMOGLU, A. OKATAN, (*Fac. Sci., Karadeniz Technical Univ., 61080 Trabzon, Turkey)

      J. Chromatogr. 609, 402-406 (1992). TLC of benzoxazolinone and 2,4-dihydroxy-1,4-benzoxyzin-3-one on silica with 1) chloroform - methanol - water 75:25:3, 2) butanol - methanol - water, 3) ethyl acetate - methanol - water 100:13:5:10, 4) chloroform - acetic acid 90:10. Detection under UV 254 nm and identification by IR and 3H NMR. Confirmation of the presence of 2-glucosyl-2,4-dihydroxy-1,4-benzoxazin-3-one also by TLC and HPLC.

      Classification: 4e, 8b
      74 094
      (Identification of residual tetracyclines in honey by TLC/FABMS
      H. OKA, Y. IKAI, J. HAYAKAWA, K. HARADA, K. MASUDA, M. SUZUKI, R. HIMEI, M. HORIE, H. NAKAZAWA, (Aichi Prefect. Inst. Pub. Health, Nagoya, Japan 462)

      Shokuhin Eiseigaku Zasshi (J. Food Hyg. Soc. of Jap.) 34, 517-523 (1993). Establishment of a confirmation method using reverse-phase TLC/FABMS for identifying residual tetracycline antibiotics. oxytetracycline, tetracycline, chlortetracycline and doxycycline in thiaglycerol as matrix.

      Classification: 4e, 28a