Cumulative CAMAG Bibliography Service CCBS
Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.
The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:
- Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
- Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
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- Search by CBS edition: Select a CBS edition and find all related publications
Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. You can export your saved items to PDF by clicking the download icon.
Phytochem. Anal. doi:10.1002/pca.3265 (2023). HPTLC of antidiabetic compounds in the leaves of Annona cherimola on silica gel with chloroform - propanol - ethyl acetate 21:2:2. Detection of α-amylase inhibitors by spraying with 4 mL of an enzyme solution (6 U/mL) in acetate buffer (0.5 M, pH 6.9), followed by incubation at 37 °C for 10 min, dipping into a 1 % starch solution for 3 s and incubation at 37 °C for 15 min. Inhibitory zones were detected by dipping into a Gram's iodine solution. Detection of α-glucosidase by spraying with 4 mL enzymatic solution (5 U/mL α-glucosidase in phosphate buffer), followed by incubation at 37 °C for 10 min. The enzyme cleaved the substrate producing α-naphthol, which was detected by spraying with 4 mL of an aqueous solution of Fast Blue B salt. Further analysis of active zones by UHPLC-DAD coupled with detector electrospray ionisation tandem mass spectrometry (ESI-MS/MS).
Trends Anal. Chem. 166, 117171 (2023). Review of the systems that employ technology and control systems to carry out tasks with minimal human intervention for sample preparation in chromatography and mass spectrometry laboratories. The papers described systems in an open-source robot allowing orthogonal hyphenation in HPTLC with LC and high-resolution MS.
Marine Drugs 20(1), 2 (2022). Samples were the products of partial vs. complete hydrolysis of agar by rGaa16Bc, a recombinant form of agarase Gaa16B from Gilvimarinus agarilyticus (Cellvibrionaceae) overexpressed in Escherichia coli. D-galactose (G) and its oligomers (neoagarobiose (NA2), neoagarotetraose (NA4), neoagarohexaose (NA6)) were used as standards. TLC on silica gel with n-butanol – acetic acid – water 2:1:1. Visualization by spraying orcinol reagent (50 mg orcine monohydrate in 100 mL acetone and 8 mL sulfuric acid), followed by 10 min heating at 110° C. The observed patterns showed the apparition of NA6 and NA4 among the hydrolytic products already after 20 min reaction, whereas NA4 and NA2 were the main products after over-night complete hydrolysis.
Phytochem. Rev. doi.org/10.1007/s11101-022-09844-x (2022). The paper discussed a prioritization approach for dereplication that focuses on the most necessary to discover as a tool to deliver experimental real-world results. The principle of planar chromatographic super-hypenations was discussed, including a workflow that combined chemistry and biology to prioritize the compounds in complex samples. The workflow consisted in 1) parallel screening and separation of multiple complex mixtures using imaging HPTLC, 2) planar multiplex bioassay for non-targeted detection of important active compounds, and 3) heart-cut elution of active zones of interest directly out of the bioautogram into orthogonal HPLC-DAD-ESI-HRMS for targeted characterization. The power of planar multiplex bioassays was described for different applications, and chances and limitations for dereplication were also discussed.
J. Chromatogr. A. 1690, 463775 (2023). HPTLC of estrogen-like and antiestrogen-like compounds in 15 rosé, white and red wine samples of different origin on RP-18 with n-hexane - ethyl acetate 4:1. Bioassay by dipping into the yeast cell suspension, followed by incubation at 30 °C for 3 h and drying for 4 min. Detection by dipping into 40 mL MUG substrate solution (16 mg MUG in 1 mL dimethyl sulfoxide and 39 mL citrate buffer) for 5 s, followed by incubation at 37 °C for 1 h and drying for 2 min. Detection at FLD 366 nm/ > 400 nm. The 10D hyphenation NP-HPTLC−UV/Vis/FLD–pYAES−heartcut–RP-HPLC–DAD–HRMS/MS allowed the detection of estrogens as well as antiestrogens in the matrix-rich wine.
J. Chromatogr. A. 1683, 463522 (2022). HPTLC of powdered root sample of Saussurea costus on silica gel with n-hexane - toluene - tetrahydrofuran 10:1:2. Planar bioluminiscent cytotoxicity assay by dipping into concentrated PBS, followed by removal of excess liquid and adding of human embryonic kidney (HEK) 293T cells expressing ELuc, which uses D-luciferin as the substrate for light emission (cell suspension of 5000 cells/μL). To avoid drying out of the plate, two stripes of paper were added to the side of the chamber, which were wetted with 1.5 mL of bidistilled water. After incubation for 6 h, the HPTLC plate was completely dried under cold air, followed by dipping twice into the respective bioluminescent substrate solution for each cell type. Dose-dependent cytotoxicity activity was calculated for the bioluminescence signal reduction.
Anal. Methods. 11, 4939-4945 (2019). HPTLC of Peganum harmala seeds on silica gel with ethyl acetate - methanol - ammonia (25%) 85:11:4. Detection under UV light at 254 and 366 nm. Track pattern application by applying each reference compound solution (physostigmine: 0.1–1.5 ng/zone; rivastigmine tartrate and piperine: 200–2000 ng/zone). A new piezoelectric spraying workflow was applied for the HPTLC-enzyme assay: the plate was sprayed with the enzyme solution (6.6 units per mL AChE or 3.3 units per mL BChE), followed by incubation at 37 °C for 25 min and spraying with 0.5 mL substrate-chromogenic solution and drying. The enzyme inhibition densitograms of both assays were measured by inverse scan at 546 nm using a mercury lamp. Equivalency of the AChE/BChE inhibition was calculated for two quantitative modes: applied and developed. A higher accuracy was obtained via an appropriate co-developed reference inhibitor for the enzyme inhibition equivalency calculation.
Anal. Chem. 94, 14554-14564 (2022). The paper describes a completely solvent-resistant 2LabsToGo system and the instructions for its configuration from readily available materials on an open-source basis. A food dye mixture and different water samples were used as proof of concept. The following features and construction of the system were described in detail: the solvent-resistant liquid dosing system, the heatable multifunctional plate holder, development, capturing bioluminescent plate images, detection and digital evaluation, and the software structure compatible with cloud-based database.