Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. The saved items can be printed to PDF using the print function of your web browser.

      126 029
      Rapid structural characterisation of benzylisoquinoline and aporphine alkaloids from Ocotea spixiana acaricide extract by HPTLC-DESI-MSn
      R. CONCEIÇAO, I. REIS, A. CERQUEIRA, C. PEREZ, M. DOS SANTOS JUNIOR, A. BRANCO, D. IFA, Mariana BOTURA* (*Department of Health, State University of Feira de Santana, Feira de Santana, Bahia, Brazil, mbbotura@uefs.br)

      Phytochem. Anal. 31, 711-721 (2020). HPTLC of 13 aporphine and four benzylisoquinoline-type alkaloids in the twigs of Ocotea spixiana on silica gel with propanol - water - ammonium hydroxide 18:2:1. Detection by desorption electrospray ionisation mass spectrometry (DESI-MS).

      Classification: 4e, 22
      126 038
      HPTLC–MS method for the determination of benzodiazepines in urine samples
      P. CHOUDHARY*, S. BANSAL, K. VERMA (*Regional Forensic Science Laboratory, Government of National Capital Territory of Delhi, 7th Floor NDMC Building, Yashwant Place, Chankyapuri, New Delhi 110021, India, pallavi.choudhary23@gmail.com)

      J. Planar Chromatogr. 33, 523-530 (2020). HPTLC of clonazepam (1), lorazepam (2), alprazolam (3), diazepam (4), furazepam (5), oxazepam (6) and nitrazepam (7) in urine on silica gel with chloroform - glacial acetic acid 9:1. Direct extraction of the substances from the plate into a mass spectrometer. The hRF values for (1) to (7) were 73, 44, 22, 64, 18, 53 and 40, respectively. Linearity was between 0.2 and 0.8 µg/mL. Intermediate precision was below 5 %. LOD was 1 ng/mL for each substance. Average recovery was 67.1 % for (1), 68.9 % for (2), 64.2 % for (3), 73.1 % for (4), 50.7 % for (5), 81.4 % for (6) and 64.4 % for (7).

      Classification: 4e, 32a
      126 052
      Validation of an analytical method for the detection of ephedrine and its analogues in forensic samples using HPTLC–MS
      K. GOYAL, N. TOMAR*, A. SINGH, R. SARIN, S. SHUKLA (*Amity Institute of Forensic Science, Amity University, Noida, Uttar Pradesh, India, tomarneh@gmail.com)

      J. Planar Chromatogr. 33, 397-404 (2020). HPTLC of ephedrine on silica gel with n-butyl acetate - acetone - 1-butanol - 5 M ammonia - methanol 4:2:2:1:1. Detection at UV 254 nm. The separated zones eluted directly from the plate into an MS. The ionization mode was selected as electrospray ionization (ESI). The hRF value for ephedrine was 41.

      Classification: 4e, 32a
      125 034
      Bioprofiling of Mexican Plectranthus amboinicus (Lour.) essential oil via planar chromatography–effect-directed analysis combined with direct analysis in real time high-resolution mass spectrometry
      A. BAÑUELOS-HERNANDEZ, E. AZADNIYA, E. RAMIREZ, Gertrud MORLOCK* (Institute of Nutritional Science, and TransMIT Center for Effect-Directed Analysis, Justus Liebig University Giessen, Giessen 35390, Germany, Gertrud.Morlock@uni-giessen.de)

      J. Liq. Chromatogr. Relat. Technol. 43, 344-350 (2020). HPTLC of hydrodistilled Plectranthus amboinicus essential oil on silica gel with n-hexane - ethyl acetate - ethanol 95:3:2. Detection by dipping into anisaldehyde sulfuric acid reagent, followed by heating at 100 ºC for 5 min. HPTLC-bioprofiling was performed using the following assays by dipping the chromatogram into the respective solution, followed by drying, incubation and documentation at white light or measuring bioluminescence: DPPH* radical reagent assay (using a 0.2 mg/mL 2,2-diphenyl-1-picrylhydrazyl solution in methanol), AChE inhibitory assay, tyrosinase inhibitory assay, alpha- and beta-glucosidase inhibitory assays, alpha-amylase inhibitory assay, Gram-negative antimicrobial bioassay (chromatogram immersion into a A. fischeri suspension), and Gram-positive antimicrobial bioassay (chromatogram immersion into a B. subtilis bacterial suspension). Direct analysis in real time mass spectrometry allowed the detection of five bioactive compounds: caryophyllene oxide (hRF 20), a-humulene (hRF 26), carvacrol (hRF 40), methyl carvacrol ether (hRF 76) and caryophyllene (hRF 84).

      Classification: 4e, 15a
      125 012
      Imaging the unimaginable: Desorption Electrospray Ionization – Imaging Mass Spectrometry (DESI-IMS) in natural product research
      D. PARROT, S. PAPAZIAN, D. FOIL, Deniz TASDEMIR* (*Research Unit Marine Natural Products Chemistry, GEOMAR Helmholtz Centre for Ocean Research Kiel, 24106 Kiel, Germany; dtasdemir@geomar.de)

      Planta Medica 84(9/10), 584-593 (2018). A review with 120 references on DESI technique coupled with MS for natural products. Paragraph on sample preparation (9 references) compares analyte desorption surfaces: either directly from the biological sample, or indirectly from surfaces on which the sample had been imprinted. Direct desorption can be performed only from samples with hard, smooth and regular surfaces, or from cryosections, which are usual for animal tissues. For plants, indirect analysis is preferable because of their wax-rich, hydrophobic, absorbent and/or irregular surfaces. Imprinting of plant organs and tissues can be performed either on glass (however with a very rapid ablation of the analytes from its surface), or on sorbent material, like TLC silica gel or porous polytetrafluoroethylene (PTFE). While PTFE layers are reported as more expensive and better in terms of reproducibility and quantitative analysis, both TLC and PTFE layers have similar performance for analyte retention until desorption.

      Keywords: herbal review
      Classification: 1, 4e, 32e
      124 042
      Quantitative TLC-SERS detection of histamine in seafood with support vector machine analysis
      A. TAN (Tan Ailing), Y. ZHAO (Zhao Yong), K. SIVASHANMUGAN, K. SQUIRE, A. WANG* (*School of Electrical Engineering and Computer Science, Oregon State University, Corvallis, OR, 97331, USA, wang@engr.orst.edu)

      Food Control. 103, 111-118 (2019). Thin layer chromatography in tandem with surface-enhanced Raman scattering (TLC-SERS) of histamine in tuna samples on diatomaceous earth plates with ethanol - ammonia 3:1. Detection by spraying with Pauly's reagent (equal mixture of 20 mM sulfanilic acid in a 1 M HCl solution and 200 mM sodium nitrite solution, followed by adding 10 % anhydrous sodium carbonate in a 5 % ethanol solution). Gold nanoparticles were deposited on the plate zone and measurements were performed using a Raman spectrometer with an excitation laser wavelength of 785 nm.

      Classification: 4e, 17a
      124 055
      Direct bioautography hyphenated to direct analysis in real time mass spectrometry: Chromatographic separation, bioassay and mass spectra, all in the same sample run
      T.T. HÄBE, M. JAMSHIDI-AIDJI, J. MACHO, Gertrud E. MORLOCK* (*Chair of Food Science, Institute of Nutritional Science, Interdisciplinary Research Center (IFZ), Justus Liebig Univ. Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany, Gertrud.Morlock@uni-giessen.de)

      J. of Chromatogr. A 1568, 188-196 (2018). Mass spectra by DART-MS were recorded directly in situ the bioautogram, immediately after direct bioautography (DB). This allowed to detect bioactive analytes within the bioautogram and discriminate microorganism cells and polar bioassay medium ingredients which could otherwise stress the MS system. DB-DART-MS was used for bioactive compounds in cosmetics using the Bacillus subtilis and Aliivibrio fischeri bioassays for detection of Gram-positive and Gram-negative antimicrobials. Planar yeast estrogen screen was used for detection of estrogen-effective compounds. HPTLC-DART-MS of parabens in hand creams either on silica gel with petroleum ether - glacial acetic acid 20:3 or on RP-18W with methanol - water 1:1. Detection under UV 254 and 366 nm. Bioassay by immersing the neutralized chromatograms into the bacterial suspensions.

      Keywords: densitometry HPTLC
      Classification: 4e, 5b
      124 062
      Rapid isolation of a dipeptidyl peptidase IV inhibitor from Fritillaria cirrhosa by thin-layer chromatography–bioautography and mass spectrometry-directed autopurification system
      L. GU, T. TIAN, L. XIA, G. CHOU*, Z. WANG (*Key Laboratory of Standardization of Chinese Medicines, Ministry of Education, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China, chouguixinzyb@126.com)

      J. Planar Chromatogr. 32, 447-451 (2019). HPTLC of Fritillaria cirrhosa on silica gel with ethyl acetate - methanol - ammonia solution - water 180:20:10:1. Bioautography by dipping into a 0.15 mg/mL solution of substrate Gly-Pro-p-nitroanilide hydrochloride in 50 % of ethanol, followed by ethanol removal in the hood and dipping into a 10 U/L DPP IV enzyme solution in TrisHCl buffer (pH 8.2, 70 mM), followed by incubation at 37°C for 40 min. Detection by dipping into a solution of 0.5 % sodium nitrite in 1.2 M hydrochloric acid, followed by drying slightly for 5 min and dipping into 0.05 % N-(1-naphthyl)ethylenediamine dihydrochloride solution. Further analysis by mass spectrometry using a TLC interface. The hRF value for the dipeptidyl peptidase IV inhibitor was 58.

      Classification: 4e, 8b