Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. The saved items can be printed to PDF using the print function of your web browser.

      Direct bioautography hyphenated to direct analysis in real time mass spectrometry: Chromatographic separation, bioassay and mass spectra, all in the same sample run
      T. T. HÄBE, Maryam JAMSHIDI-AIDJI, Jennifer MACHO, Gertrud E. MORLOCK* (*Chair of Food Sci., Inst. of Nutrit. Sci., and Interdiscipl. Res. Center (iFZ), Justus Liebig Univ. Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany,

      J. Chromatogr. A 1568, 188-196 (2018). Application of an advantageous combination, the desorption-based direct analysis in real time mass spectrometry (DART-MS) immediately after direct bioautography (DB), i.e., in the presence of microorganisms, bioassay medium and substrate reagent. The method offers a straightforward and efficient mass spectrometric detection of bioactive analytes within the bioautogram. It discriminated microorganism cells and highly polar bioassay medium ingredients which could otherwise stress the MS system. Investigation of DB-DART-MS for bioactive compounds in cosmetics using the Bacillus subtilis and Aliivibrio fischeri bioassays for detection of Gram-positive and Gram-negative antimicrobials, respectively, and the planar yeast estrogen screen for detection of estrogen-effective compounds. Study of the influences of three different bioassay matrices on the analyte response and DB-DART-MS performance on different layers (NP and RP) on the example of parabens in hand creams. Ion suppression was enhanced with increasing culture medium complexity. The mass spectrometric quantification by DB-DART-MS at the ng-level in situ each different bioautogram was verified by comparison to HPTLC-DART-MS. The total paraben content of hand creams 1 and 2 was 0.17–0.20% and 0.30–0.34%, respectively, depending on the method used. It proved that DB-DART-MS is a reliable qantitative bioanalytical hyphenation.

      Keywords: hptlc cosmetics
      Classification: 4e
      Effect-directed profiling of aqueous, fermented plant preparations via high-performance thin-layer chromatography combined with in situ assays and high-resolution mass spectrometry
      Maryam JAMSHIDI-AIDJI, Jennifer MACHO, Margit MUELLER, Gertrud MORLOCK* (*Institute of Nutritional Science, Interdisciplinary Research Center (IFZ), Justus Liebig University Giessen, Heinrich-Buff-Ring 26–32, 35392 Giessen, Germany, )

      J. Liq. Chromatogr. Relat. Technol. 42, 266-273 (2019). HPTLC of aqueous, fermented plant preparations from Chamomilla recutita L. (1), Allium cepa L. (2), Equisetum arvense L. (3) and Hamamelis virginiana L. (4) of different harvest years on silica gel with ethyl acetate - toluene - formic acid - water 16:4:3:2. The method was combined with effect-directed analysis (EDA) and high-resolution mass spectrometry (HRMS). For α-/β-glucosidase assays, the plate was sprayed with 2 mL substrate solution (60 mg 2-naphthyl-α-D-glucopyranoside or 2-naphthyl-β-D-glucopyranoside in 50 mL ethanol), then sprayed with 1 mL sodium acetate buffer and 2 mL enzyme solution (500 units α-glucosidase), followed by incubation at 37 ºC for 10 min. Analysis of multi-potent compounds was also performed using the 2,2-diphenyl-1-picrylhydrazyl reagent and Gram-positive Bacillus subtilis assays, followed by recording of elution head-based HPTLC-ESI-HRMS spectra. 


      Classification: 4e
      Diterpene lipo-alkaloids with selective activities on cardiac K+ channels
      T. KISS, B. BORCSA, P. ORVOS, L. TÁLOSI, J. HOHMANN, D. CSUPOR* (*Department of Pharmacognosy, Faculty of Pharmacy, University of Szeged, Szeged, Hungary;

      Planta Med. 83(17), 1321-1328 (2017). Benzoyl-aconine esters (lipo-alkaloids) produced by transesterification of aconitine (isolated from Aconitum sp.) with long-chain fatty acids were purified by a multistep chromatographic method, including cyclodextrane gel filtration chromatography, centrifugal planar chromatography on aluminium oxide layer using cyclohexane – chloroform – methanol 70:30:1 followed by 70:30:3 and/or preparative thin-layer chromatography on aluminium oxide layer with toluene – acetone – ethanol – concentrated ammonia 70:40:10:3.

      Classification: 32e, 22, 11a, 4d, 4e
      100 017
      Structural characterization of gangliosides by HPTLC/IR-MALDI-o-TOF
      K. DREISEWERD, J. MUETHING* (*Institut für Medizinische Physik und Biophysik, Westfälische Wilhelms-Universität Münster, Rbert-Koch-Str. 31, 48149, Germany;

      CBS 97, 2-5 (2006). HPTLC of gangliosides on silica gel with chloroform - methanol - water 24174 and addition of 2 mM CaCl2, after chamber saturation with filter paper for 3 h, over 80 mm, followed by drying for 5 min at room temperature. Detection by dipping in orcin solution (0.3 % (w/v) in 3 M H2SO4) followed by heating at 100 °C for 3 min. Alternative detection of GM3-bands by derivatization with primulin (0.02 % (w/v) in aceton - water 41). Quantitative determination by direct IR-MALDI-o-TOF-analysis. The limit of detection for GM3 was about 50 ng/zone.

      Classification: 4e
      103 025
      The new TLC-MS interface
      M. LOPPACHER*, R. ROLLI (*CAMAG, Sonnenmattstr. 11, 4132 Muttenz, Switzerland, matthias.

      CBS 102, 2-3 (2009). A semi-automatic interface for hyphenation of TLC with mass spectrometry, which was based on the ChromeXtractor by Luftmann, is introduced. The interface is connected to a HPLC pump and the MS. By means of an extraction piston any target zone can be eluted from a TLC/HPTLC plate directly into the MS. Example for identification of the zone at hRf 15 in a standard mixture of caffeine, paracetamol, and acetylsalicylic acid the mass spectrum of the zone is recorded. After subtraction of a background spectrum a mass spectrum free from system peaks is obtained, which shows mainly substance signals - here the mass signal m/z 195 [M+H]+ for caffeine.

      Keywords: hptlc
      Classification: 4e
      104 017
      Analysis of human plasma lipids and soybean lecithin by means of high-performance thin-layer chromatography and matrix-assisted laser desorption/ionization mass spectrometry
      G. STUBIGER, E. PITTENAUER, O. BELGACEM, P. REHULKA, K. WIDHALM, G. ALLMAIER* (*Institute of Chemical Technologies and Analytics, Vienna University of Technology, 1060 Vienna, Austria,

      Rapid Commun. Mass Spectrom. 23, 2711-2723 (2009). HPTLC in combination with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was used for the analysis of complex lipid mixtures. For the separation of lipids one-dimensional HPTLC on silica gel aluminum foil was used with a two-phase mobile phase. The combination with MALDI-MS allowed the identification of 70 distinct lipid species and the analysis of even minor lipid classes from only very small volumes of human plasma (50 µL).

      Classification: 4e
      110 023
      Free radical scavenging activities of polyphenolic compounds isolated from Medicago sativa and Medicago truncatula assessed by means of thin-layer chromatography DPPH rapid test
      L. CIESLA*, I. KOWALSKA, W. OLESZEKA, A. STOCHMALA (*Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation – State Research Institute, 8 Czartoryskich Street, 24–100 Pulawy, Poland,

      Phytochem. Anal. 24, 47-52 (2013) TLC of 22 acylated phenolic compounds on silica gel with acetonitrile - water - chloroform - formic acid 12321, followed by dipping into 0.2 % methanolic 2,2-diphenyl-1-picrylhdrazyl solution (DPPH radical reagent) for 5 s and kept in the dark for 30 min. Free radical scavenging activity of the acylated phenolic compounds was assessed by coupling with the image processing software ImageJ .

      Classification: 4e
      114 010
      Science meets regulation
      Anna R. BILIA* (*Department of Chemistry, University of Florence, Sesto Fiorentino, Fl 50019, Italy,

      J. Ethnopharmacol. 158, 487-494 (2014). The review described special techniques such as DNA fingerprinting, nuclear magnetic resonance, near infra red and (bio)sensors that combined with chromatographic techniques provide complementary analytical methods able to give rapid analysis responses, to operate directly on complex matrices, to be portable and to have fast analysis times. TLC is mentioned in the identification section, HPTLC is mentioned in the test for aristolochic acids in herbal drugs.

      Classification: 4e