Trends Anal. Chem. 90, 1-13 (2017). Review of surface-enhanced Raman spectroscopy combined with other techniques to improve characterization, including TLC-SERS to improve SERS sensitivity by eliminating interference from a mixture. TLC-SERS application included discriminating contaminants or toxicants in real-life samples, drugs and biomarker in body fluids, synthetic dye mixtures, and products of a chemical reaction system.

root extract via HPTLC–UV/Vis/FLD–EDA–MS. J. Planar Chromatogr. 31, 79-86 (2018). HPTLC with effect-directed analysis (EDA) for (bio)profiling of Pimpinella saxifraga root. HPTLC on silica gel with 1) ethyl acetate – methanol – acetic acid 8:3:1 for polar separation, and 2) toluene – ethyl acetate – acetic acid 20:5:1 for apolar separation. Ten different detections were shown on one HPTLC plate cut into sections. Detection of absorbing compounds under A) white light and B) UV 254 nm and C) fluorescent ones under UV 366 nm. Three plate sections were used for microchemical derivatizations for D) detection of flavonoids after derivatization with diphenylborinic acid 2-aminoethylester reagent, followed by dipping into a polyethylene glycol 400 solution, E) universal detection with anisaldehyde sulfuric acid reagent, and F) detection of glycosides with diphenylamine aniline reagent. Effect-directed detection of G) DPPH scavenging compounds, antimicrobials via H) A. fischeri and I) B. subtilis bioassays, and J) AChE inhibitory compounds was also performed. Multi-detection showed multi-potent zones with hRF values of 19, 24, 49 and 78, which were exemplarily further characterized by HPTLC–ESI–MS in both ionization modes. A weak estrogen-effective zone was also observed via HPTLC–planar yeast estrogen screen (pYES) bioassay on RP-18 with n-hexane – toluene – ethyl acetate 6:3:4 or, after optimization, toluene – ethyl acetate 1:1.

Phytochemistry 155, 37-44 (2018). HPTLC of pine resins collected from Abies grandis, Pseudotsuga menziesii, Picea abies, Pinus sylvestris L. and Pinus strobus L. in a forest area of The Netherlands during early and late spring on silica gel with toluene – isopropyl alcohol – diethyl ether 16:1:3. Detection by spraying with 2 mL of anisaldehyde sulfuric acid solution, followed by heating at 100 ºC for 3 min. Qualitative identification under UV light at 366 nm. Most of the metabolites detected in GC-MS were confirmed by HPTLC analysis. HPTLC proved to be an important technique for metabolic profiling.

Chromatographia 21, 234-238 (1986). TLC of ecdysteroids on silica with a) dichloromethane - ethanol 85:15, b) ethyl acetate - methanol - NH3 85:10:5, c) ethyl acetate - methanol - water 8:2:1. Detection under UV at 254 nm or spraying with vanillin reagent.

Part 2. Application of fluorescence-based spectroscopic techniques for off-line detection. Anal. Chim. Acta 193, 193-207 (1987). Identification and determination of the compounds eluted from LC and collected on a TLC plate by direct flurescence emission, fluorescence excitation emission spectra and fluorescence line-narrowing spectroscopy with tetracene and benz(K)fluoranthene as model compounds. Discussion of the possibility of widening the application of the technique. Detection limit, pg level for very selective narrow-line fluorescence.

J. China Pharm. Univ. 18, 205-206 (1987) (Zhongguo Yaoke Daxue Xuebao). TLC on silica with heptane - ethyl acetate - ethanol 5:5:1. Detection by spraying with 0.5M sulfuric acid. Elution with ethanol. Quantification by UV spectrophotometry.

J. Chromatogr. 442, 420-423 (1988). TLC of N- (4-aminobenzoyl)-g-oligo (L-glutamic acid)S on silica with methanol - 25% NH3 - acetic acid 15:2:1. Detection by spraying twice with 1% triethylamine in acetone and 0. 01% fluorescamine in acetone and viewing under UV 366 nm. Elution with potassium phosphate buffer (pH 7. 0). Quantification by fluorescence spectrophotometry at 400 nm excitation and 490nm emission.

J. Chromatogr. 488, 129-143 (1989). Outline of various neurochemical isolation procedures for the analysis of several monoamine neurotransmitters/neuromodulators and their principal oxidatively deaminated metabolites. TLC of dansyl derivatives of trace amines on silica with various solvent systems. Identification and quantification by MSEI ionisation followed by selected-ion monitoring of their molecular ions with deuterated homologues as internal standards.