Biochem. Biophys. Res. Commun. 378, 224-229 (2009). TLC of the hydrolytic action patterns of the purified Nostoc punctiforme debranching enzyme on the following substrates pullulan, amylopectin, soluble starch, amylose, cyclodextrins, and maltooligosaccharides (form glucose to maltoheptaose), on silica gel with 1-propyl alcohol – ethyl acetate – water 623. Detection by dipping into 0.3 % N-(1-naphthyl)-ethylenediamine and 5 % sulfuric acid in methanol, followed by heating for 10 min at 110 °C. Quantitative determination by radioactivity measurement of the 14C-labeled maltooligosaccharides.

assay for cellulolytic enzymes. Anal. Biochem. 150, 264-272 (1985). TLC of the title compound on silica with ethyl acetate - water - methanol 834. The method facilitates analysis of the activity of cellulolytic enzymes. Also separation of cellooligosaccharides using other adsorbents, solvents and impregnants.

Anal. Biochem. 269, 410-417 (1999). Quantitative analysis of glutathione (GSH) or of glutathione S-Transferase (GST) activity using a new fluorescent molecule, 5-(pentaflourobenzoylamino)fluorescein (PFB-F). TLC of the formed glutathione adduct, GS-TFB-F, and of excess PFB-F on silica gel 60F254 with 1-butanol - methanol - water 311. The GS-TFB-F adduct spots were quantified with a fluorescence plate scanner using the blue fluorescence/chemifluorescence (480 nm) mode. Detection limits for GSH and for GST activity were 10 pmol/(l and 1 ng/(l, respectively.

J. Planar Chromatogr. 22, 395-398 (2009). HPTLC of coenzyme Q10, cholesterol and biological extracts on silica gel with petroleum ether - diethyl ether - acetic acid 85151. Detection by dipping into 5 % phosphomolybdic acid in ethanol for 10 s followed by heating for 10 min at 110 °C. Quantitative evaluation in visible light.

J. Chromatogr. 382, 314-320 (1986). TLC of xanthine dehydrogenase on silica - cellulose with propanol -7% NH3 21. Measurement by fluorodensitometry at 366 nm.

Anal. Biochem. 286, 297-300 (2000). 1-ml aliquots of reaction mixtures were quenched by loading directly on silica gel TLC plates. Separation of residual glucose-1-phosphate from polymeric glucans with n-butanol - isopropanol - acetic acid - water 31244. Visualization by spraying or dipping with acetic acid - H2SO4 - anisaldehyde 10021.

J. of Chromatogr. A 1218 (20), 3089-3084 (2011). Comparison of the separation of the structurally related angiotensin-converting enzyme (ACE) inhibitors lisinopril, cilazapril, ramipril and quinapril and their corresponding active diacid forms (prilates) by conventional TLC on silica gel with the separation on monolithic ultra-TLC (UTLC) phase. Technical modifications of the commercially available equipment for sample application, development and detection were necessary for the use with UTLC plates. Development in a modified horizontal developing chamber with ethyl acetate - acetone - acetic acid - water 16412. Detection by absorbance measurement at 220 nm and after exposure to iodine vapors under daylight, as well as by image analysis. As a result the monolithic layer was more efficient for the separation of structurally similar polar compounds, such as prilates, than conventional silica layers. Confirmation of the identity of the compounds by ESI-MS after their online extraction from the UTLC and TLC plates.

J. Planar Chromatogr. 1, 146-149 (1988). TLC on silica with two different solvent mixtures containing amines or acids. Quantification by densitometry at 265 nm. Discussion of the chromatographic behavior of malonyl-CoA, acetyl-CoA, CoA, AMP, ADP and ATP in the systems.