Biochem. Biophys. Res. Commun. 470, 168-174 (2016). HPTLC of glycosphingolipid during the evaluation of enzyme activities of human Gb3/CD77 synthase supplemented with UDP-[14C]Gal on silica gel with chloroform – methanol – water 11:9:2. Detection of radiolabeled reaction products by exposure to phosphor screens for 8 weeks in -80 °C, followed by scanning. In parallel staining was used to facilitate identification of bands on the phosphor screens by spraying the plates with orcinol (0.2 %) in 3 M aqueous sulfuric acid, followed by heating at 110 °C for 10 min.

Anal. Biochem. 200, 27-30 (1992). TLC of the substrate from products of [14C] bilirubin formation in a coupled enzyme as involving hemo oxygenase and biliverdin reductase actions. Quantification by liquid scintillation counting of radioactive material on chromatograms. Also autoradiography.

J. Agric. Food Chem. 64, 8838-8847 (2016). HPTLC of free fatty acids produced after treatment of vegetable oils with crude lipase extracts from germinated seeds of Adansonia suarezensis, Adansonia grandidieri, Moringa oleifera, Moringa drouhardii, Jatropha mahafalensis, and Jatropha curcas seeds on silica gel with hexane – diethyl ether – acetic acid 70:30:1. The hRF value of free fatty acid was 43.

Contribution to the investigation of 7,8-dihydropteroate synthase. J. Chromatogr. 659, 185-189 (1994). Evaluation of the 7,8-dihydropteroate synthase reaction via determination of the stable pteroic acid from oxidation of the enzymatic product dihydropteroic acid. TLC on amino-bonded silica with a solution of 52% acetonitrile in 100 mM tris-HCl buffer (pH 8.6). Quantification by densitometry. Detection limit: at levels down to 0.5 ng/µL.

Phytochem. Anal. 28, 74-86 (2017). Review of the latest developments and applications in the field of bioautographic enzyme analysis and assays including acetylcholinesterase, dipeptidyl peptidase 4, glucosidase, lipase, xanthine oxidase, glucose-6-phosphate-dehydrogenase, phosphoglucose isomerase and tyrosinase.

Chinese J. Chromatogr. (Sepu) 12, 380-381 (1994). TLC of lactic acid dehydrogenase (LDH) and LDH isozyme on polyacrylamide with Tris-Gly system (0.05 mol/L, pH 7.9). Detection by densitometry. Investigation of the periodic variation by comparison of the finger prints of the samples sampled during hedgehog hibernation.

J. Planar Chromatogr. 31, 169-172 (2018). HPTLC for the analysis of enzyme activity of the purified β-glucosidase from Cyamopsis tetragonoloba with natural isoflavonoid (genistin and daidzin) and flavonoid O-glycoside (rutin, naringin and hesperidin) substrates on silica gel with toluene – ethyl acetate – acetone – formic acid 20:4:2:1 for genistin, daidzin and rutin, methanol – chloroform 3:17 for hesperidin and methanol – ethyl acetate 3:7 for naringin. Detection under UV 254 nm. The hRF values were 18 and 32 for daidzein and genistein, respectively. TLC was successfully used as the first step for validation of the metabolic product formation.

J. Planar Chromatogr. 7, 372-375 (1994). TLC of the technical products from the synthesis of the title substance on silica with methanol - pyridine - formamide 80:15:5. Detection by inhibition of choline esterase: spraying the plates first with enzyme solution, and after incubation for 30 min at room temperature, with the mixed substrate - DTNB solution. After use of ninhydrin, drying for 10 min and heating at 120 °C red and reddish-blue spots appear on a pink background.