PLoS ONE 18(10), e0292514 (2023). Samples were 8 glycolipids, either sialylated or not; the two main examples were monosialotetrahexosylganglioside (GM1) and gangliotetraosylceramide (= asialo-GM1 = ASGM1). TLC on silica gel with chloroform – methanol – water – acetic acid 300:200:30:1, followed by air-drying. Visualization of glycolipids by spraying 5-methylresorcinol solution (0.2 % in 2 M sulfuric acid), followed by 5 min heating at 110°C. For immunostaining, underivatized chromatograms were immersed into a blocking gel (1% gelatin, 1% polyvinylpyrrolidone and 1mM EDTA in phosphate-buffered saline solution (PBS)), followed by immersion into a solution of monoclonal (50 ng/mL) or polyclonal (1:1,000) anti-ASGM1 antibodies purposedly produced in rabbits. After 1h reaction at room temperature, the layers were washed thrice with T-PBS (0.05 % Tween 20 in PBS) and horseradish-peroxidase-conjugated anti-rabbit IgG (1:40,000) was added. After washing thrice with T-PBS, the TLC sheets were incubated in substrate solution (tetramethylbenzidine). Blue spots indicated the glycolipids bound by the antibody. In this TLC assay, each of the five monoclonal antibodies (as well as the polyclonal serum) was specific to ASGM1 (unsialylated), whereas in ELISA (enzyme-linked immunosorbent assay) at the same concentrations three of them displayed partial cross-reactivity towards GM1, GM3 or GD1b, which are sialylated glycolipids. 

J. Sep. Sci. 46, 2300368 (2023). Review of chromatographic methods for the analysis of polysaccharides, including TLC and HPTLC. The paper described general process and fully automated techniques, mobile phases and detection methods.

Tecnología Química (RTQ) 35, 181-192 (2015). TLC of inulin in Tuna on silica gel with butanol - isopropanol - water - acetic acid 7:5:4:2. Detection by spraying with a solution of aniline - diphenylamine - phosphoric acid - acetone 1:1:5:50, followed by heating at 85 °C for 10 min. The hRF value for inulin was 87.

Nippon Kingakkai Kaiho (Jap. J. Fungus) 35, 181-188 (1994). TLC of methylated saccharides on diatomaceous earth with isopropanol - ethyl acetate 7:13. Detection by spraying with anthrone - sulfuric acid reagent. The results were compared with those obtained on Kieselguhr G.

J. Agric. Food Chem. 64, 9203-9213 (2016). HPTLC of transglycosylated products of epigallocatechin gallate using dextransucrase on silica gel with acetonitrile – water 17:3. Detection by dipping into a mixture of 0.5 % N-(1-naphthyl)ethylenediamine and 5 % sulfuric acid in methanol, followed by heating at 125 °C for 5 min.

J. Chromatogr. A 756, 211-217 (1996). TLC on silica by multiple development with 1-propanol - water mixtures. Investigation of the influence of the solvent composition, the number of developments and the solvent front on the separation of the oligomers. Detection under UV 235 nm, dipping twice in sulfuric acid - water - acetone 1:1:18 for 3 s and heating for 5 min at 80°C, then dipping twice for 3 s in a 0.5% metahydroxydiphenyl reagent in acetone and heating again for 10 min at 100°C. Densitometry at 525 nm. Saturated oligogalacturonic acids up to the nonamer and unsaturated ones up to a degree of polymerizations of 13 were separated five times with 1-propanol - water mixtures in the ratio 14:9 to 28:11.

J. Ethnopharmacol. 210, 408-416 (2018). HPTLC of inulin in a methanolic extract of Inulina racemosa on silica gel with butanol – acetic acid – water 63:27:10. Detection by spraying with 20 % sulfuric acid reagent. Quantitative determination by absorbance measurement at 297 nm. The hRF value of inulin was 71.

J. Chinese Herb Med. (Zhongcaoyao) 29, 219-221 (1998). TLC on silica gel containing 0.15 mol/L Na2HPO4, with acetone - water 24:1. Also GC. Discussion of the composition of QA2 according to TLC and GC results.