(Chinese). Chinese J. Adv.in Biochem.& Biophys. 2, 64-66 (1987) (Shengwu Huaxue Yu Shengwu Wuli Jinzhan). TLC on cellulose with n-butanol - acetic acid - 2M NH4OH 2:3:1. Detection under UV at 260 nm. Identification by comparison of Rf values to standards.

J. Planar Chromatogr. 23, 46-49 (2010). HPTLC of polysaccharides (with galactose, glucose, mannose, arabinose, ribose, xylose, rhamnose, galacturonic acid, and glucuronic acid as standards) before and after hydrolysis on silica gel with chloroform - n-butanol - methanol - water - acetic acid 9:25:10:3:3 with chamber saturation for 30 min at room temperature. Detection by spraying with aniline - diphenylamine - phosphoric acid reagent and heating at 130 °C for 10 min. Quantitative determination by absorbance measurement at 380 nm. Ninhydrin reagent was used for detection of other compounds.

J. of Medicinal Chemistry 32, 16-23 (1989). TLC of various new nitrosoureido derivatives of di- or trideoxy sugars on silica with dichloromethane - methanol 95:5 and hexane - ethyl acetate 4:1. Visualization under UV 254 nm.

J. Planar Chromatogr. 24, 211-213 (2011). HPTLC of exo-polysaccharides from bacterial fermentation and a series of mono-, oligo-, and polysaccharide reference solutions on silica gel with chloroform - toluene - 35 % formic acid - methanol 10:2:2:7 in a twin-trough chamber saturated for 1 h. Detection of starch-type polysaccharides by placing the plate into a twin-trough chamber saturated with iodine vapor. After drying determination by densitometry in absorbance mode at 400 nm. The plates were then immersed for 1 min in a solution of 10 % concentrated sulfuric acid in n-propanol - toluene 1:1, followed by heating at 115 °C for 10 min. The zones were evaluated visually and densitometrically at 400 nm.

Herba Hungarica 28, 87-91 (1989). TLC of glucose, galactose and mannose on silica with butanol - pyridine - benzene - water 5:4:3:1. Visualization with p-anisidine phtalate solution. Two-dimensional TLC of alanine, glycine and threonine on silica with butanol - acetic acid - water 4:1:1 and phenol - water 75:5. Visualization by spraying with ninhydrin.

J. Sep. Sci. 36, 1-19 (2013) The application of TLC in the characterization of polysaccharides from medicinal plants and fungi were reviewed. Physicochemical and structural characterization as well as fingerprinting methods were also described.

J. Chromatogr. 462, 471-474 (1989). TLC of dansylhydrazine derivatives of unsaturated disaccharides on silica with propanol - isopropanol - butanol - water 30:45:5:20, containing 0.04M sodium chloride and 0.01M NH3. Detection under UV. Detection limit, 90 p mol.

CBS 108, 9-11 (2012). HPTLC of fructose, galacturonic acid, rhamnose, xylose, and galactose in samples of agar agar, arabic gum, carubin, guaran, traganth, xanthan, pectin, alginic acid, alginates and carrageen on silica gel with i-propyl acetate - ethyl acetate - methanol - water 50:40:10:1 to a migration distance of 60 mm. Densitometric absorption measurement at 370 and 630 nm and peak area evaluation by linear or polynomial regression. Detection by immersion in aniline diphenylamine o-phosphoric acid reagent (20 % o-phosphoric acid (85 %) added to 1:1 mixtures of 2 % solutions of diphenylamine and aniline in acetone) and heating at 110 °C for 5 min. Comparison of HPTLC with GC gave comparable results, however, the HPTLC method is more effective in terms of sample throughput, robustness, costs and analysis time (8 times faster than GC).