Planta med. 68, 181-182 (2002). TLC of 1-methoxyphaseollidin, isowighteone, and vogelin A, B, C on silica gel. Detection under UV light at 254 nm and with vanillin-sulfuric acid reagent. Detection of 1-methoxyphaseollidin and isowighteone by a direct TLC bioautographic assay with LB (Luria-Bertani) medium.

Anal. Chem. 74, 6216-6223 (2002). A combined surface sampling probe/electrospray emitter was used for the direct readout of TLC plates by electrospray MS. HPTLC of methylene blue, crystal violet, and rhodamine 6G on RP-18 with methanol – tetrahydrofuran 32 containing 50-100 mM ammonium acetate, followed by MS detection in positive ion mode. HPTLC of fluorescein, naphtholblue black, and fast green FCF on PR-18 with methanol – water 73, followed by MS detection in negative ion mode. Drying by heating at 110°C for 30 min in an oven. Acquisition of mass spectra of components of individual bands was shown by manual stepping to and sampling from specific locations within the bands. Computer-controlled scanning of lanes was illustrated by using multiple ion monitoring in positive and negative ion modes. Readout resolution, the limits of scan speed, detection levels, TLC phase (restricted to nonpolar phases), and eluting solvents were investigated.

Anal. Bioanal. Chem. 389, 827–834 (2007). A total extract of hen egg yolk is used as phospolipid mixture to demonstrate the capabilities. TLC of phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, phosphatidylethanolamine, lysophosphatidylethanolamine and phosphatidylinositol on silica gel with chloroform - ethanol - water - triethylamine 5:5:1:5. Detection under UV 366 nm after spraying with primuline (Direct Yellow 59) reagent. Direct coupling MALDI-TOF MS and TLC can be easily implemented on commercially available MALDI-TOF devices. Roughly clean spectra without major contributions from fragmentation products and matrix peaks were obtained. This approach was sensitive enough to detect the presence of phospholipids at levels of less than 1 % of the total extract.

Rapid Commun. Mass Spectrom. 23, 2597-2604 (2009). RP-TLC of pharmaceutical formulations containing paracetamol, ephedrine, codeine, and caffeine on hydrocarbon-impregnated silica with methanol - water 1:1. Detection by desorption electrospray ionization (DESI) combined with ion mobility mass spectrometry (IM-MS). The limit of detection was 9, 16, 34, 239 and 225 µg/cm2 for codeine, caffeine, ephedrine and paracetamol, respectively.

Rapid Commun. Mass Spectrom. 25, 2619–2626 (2011). HPTLC of cardiolipins in hepatocyte sample on silica gel with chloroform - ethanol - water - triethanolamine 30:35:7:35. Qualitative determination by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Caution is required if cardiolipin is analyzed directly from the total lipid extract because phospholipid dimers may be interpreted as cardiolipins.

Chem. J. of Chinese Univ. 35 (4), 741-745 (2014). TLC is a rapid, simple, efficient, economic and widely used method with great advantages but some limitations in the detection of unknown groups. IR is a universal detection technique with advantages in the detection of colorless, non fluorescent substances. For successful application of TLC/FTIR the selection of the proper stationary phase is important. In this study sulfur fine particles were successfully employed as the coating for narrow band plates. Sulfur fine particles with the average size of 500 nm were produced by reaction of sodium thiosulfate with hydrochloric acid and characterized by XRS. Narrow band plates were coated by precipitation/evaporation technique. TLC of the mixture of rhodamine B and gentian violet on narrow band plates with tetrahydrofuran – methanol – ammonia 16:2:1 with chamber saturation for 10 min. TLC of the mixture of rhodamine B and bromophenol blue on narrow band plates with ethyl acetate – methanol – ammonia 16:1:3 with chamber saturation for 10 min. Both systems achieve complete separation. In situ detection of the separated zones by microscopic reflection infrared spectroscopy. The resulting spectra of the separated zones of rhodamine B, gentian violet and bromophenol blue matched those of the corresponding standards. There were no interfering absorption peaks except for the absorption peak of CO in the mid infrared region. The layer had a considerable ability for separation of mixed samples, therefore, the new type of stationary phase can meet the requirements for analyzing some mixtures by TLC/FTIR hyphenated technique.

Phytochem. Anal. 27, 222-228 (2016). HPTLC fingerprinting of luteolin, quercetin and their corresponding glycosidic derivatives luteolin-7-O-glucoside and rutin (quercetin-3-O-rutinoside) in Soldanella alpina on silica gel with formic acid – water – ethyl methyl ketone – ethyl acetate 1:1:3:5. A solution of 2,5-dihydroxybenzoic acid in 70 % methanol (100 mg/mL) was used as a matrix for HPTLC-MALDI-TOF MS analysis.

Rev. Bras. Farmacogn. 27, 13-39 (2017). HPTLC fingerprint of Stevia rebaudiana roots on silica gel with n-hexane – ethyl acetate 19:1. Detection by spraying with 4 % vanillin sulfuric acid reagent, followed by heating at 150 °C for 2–4 min. Chromatographic profiling by TLC and GC–MS analysis showed that roots of plants grown in vitro have the ability to produce secondary metabolites, which can be similar to those produced by plants in nature, but in a sustainable way.