Vergleich UV/Vis und IR. (Spectral detection in thin layer chromatography - Comparison UV/Vis and IR). Dünnschicht-Chromatographie InCom Sonderband 1996, 1-13. Description of spectral UV/Vis detection and direct HPTLC-FTIR-in situ measurement, in particular for investigation of mixtures where spectral detection can be used directly for quantification. Discussion of detection limits and possibilities for identification. Substances chromatographed: 7,7'-diethylamino-4-methylcoumarin, perazine, caffeine, nitrazepam.

J. Chromatogr. Sci. 33, 417-425 (1995). Quantitative hydrocarbon group type analysis of a deasphalted heavy oil from petroleum and its derived hydrocracking products by configuration of detector, an automatic sample spotter, a careful selection of the operating parameters. Use of the method for the detection and quantification of saturates, alkylaromatics, aromatics, polar and an uneluted fraction. Discussion of the repeatability and the linearity range for each separated fraction.

J. AOAC Int. 81, 329-332 (1998). Critical evaluation of the present state of hyphenated techniques in thin-layer chromatography, enumeration of possible advantages and disadvantages of the newest developments, and prerequisites for the successful application of the newest results. Compilation and evaluation of methods coupling TLC to other analytical procedures - such as GC, MS, Raman spectroscopy, Fourier transformation infrared spectrometry (FTIR), square-wave stripping voltammetry, solid-phase nuclear magnetic resonance spectroscopy, atomic absorption spectrometry, and HPLC.

J. Chromatogr. 813, 145-152 (1998). 2-D TLC on silica gel with 1) ethyl acetate for the 1st direction and 2) methanol for the 2nd. Detection by FTIR with the help of the Gram-Schmidt procedures. Identification by calculating quasi-absorbance spectra and comparison with the reference spectra of the library. Quantitation by densitometry at 230 nm. Detection limit and quantitation down to 0.05%. Precision 4.1% for demoxepan, 1.3% for nordazepam, 4.1% for aminochlorobenzophenone.

J. Chinese Trad. & Herb. Drugs (Zhongcaoyao) 32 (5), 456 -458 (2001). TLC on silica gel with 1) petroleum ether (60-90°C) - ethyl acetate 9:1, 2) benzene - ethyl acetate 17:3. Detection 1) by spraying with 2,4-dinitrophenylhydrazine - ethanol reagent, 2) by spraying with potassium iodobismuthate reagent. Also microscopy and UV spectra.

CBS 93, 14-15 (2004). HPTLC of heterocyclic aromatic amines on silica gel WRF (prewashed with methanol by chromatography followed by drying at 120 °C for 30 min) with a 6 step gradient with diethyl ether, methanol and chloroform, with alkaline conditioning via gas phase over max. 60 mm. Quantitative determination by multi-wavelength scan in absorbance mode at 252, 262, 316 nm and fluorescence measurement at 366/>400 nm. Polynomial evaluation via peak area and height. Limit of quantitation is between 1-45 ng absolute on the plate. Online coupling with MS by use of a new extractor developed by Luftmann.

Rapid Commun. Mass Sp. 19, 3659-3655 (2005). HPTLC of a preparation of neutral Stx1-binding glycosphingolipids from human erythrocytes, comprising 21.4 % and 59.1 % of the high-and low-affinity Stx1-binding ligands Gb3Cer/CD77 and Gb4Cer respectively and their antibody positive bands on silica gel with chloroform – methanol – water 15:30:4. Crude extracts were used without any further purification for an analysis by nanoelectrospray ionization quadrupole time-of-flight mass spectrometry in the negative mode. Only analytical quantities in the microgram scale of a single glycosphingolipid species are required for the structural MS characterization.

Anal. Chem. 76, 6484-6491 (2004). TLC on silica gel with chloroform - methanol - 0.2 % calcium chloride 11:9:2 or chloroform - 2-propanol - 50 mM potassium chloride 10:67:23 for separation of glycolipids, oligosaccharides, lipids, and compounds of environmental and pharmaceutical interest with coupling to an external ion source MALDI-Fourier transform (FT) MS instrument without compromising mass accuracy and resolution of the spectra. Furthermore, when the FTMS, with >70 000 resolving power, has a vibrationally cooled MALDI ion source, fragile glycolipids can be desorbed from TLC plates without fragmentation, even to the point that desorption of intact molecules from “hot” matrixes such as a-cyano-4-hydroxycinnamic acid is possible. TLC of whole brain gangliosides derivatized with orcinol-sulfuric acid reagent. The positions of the fractions on a parallel developed plate were determined; the TLC plates were attached directly to the MALDI target using double-sided adhesive tape or the strip cut from TLC plate. Different matrices were tested.