Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      130 141
      Two-dimensional high-performance thin-layer chromatography for the characterization of milk peptide properties and a prediction of the retention behavior – a proof-of-principle study
      M. TREBLIN, T. VON OESEN, L.-C. CLASS, G. KUHNEN, I. CLAWIN-RÄDECKER, D. MARTIN, J. FRITSCHE, S. ROHN* (*Department of Food Chemistry and Analysis, Institute of Food Technology and Food Chemistry, Technical University of Berlin, Berlin, Germany; rohn@tu-berlin.de)

      J Chromatogr A 1653, 462442 (2021). Samples were peptides obtained through tryptic hydrolysis of the 5 most abundant milk proteins: α-lactalbumin (α-LA), β-lactoglobulin (β-LG), α-, β- and κ-casein (CA). As standards, synthetic whey and pea (Pisum sativum, Fabaceae) peptides (selected based on the in silico tryptic digest of α-LA, β-LG, legumin A, and vicilin with one or zero miscleavages) were only used in the last assay for prediction of the RF values of peptides with known amino-acid (AA) sequences. Two-dimensional HPTLC on silica gel (pre-washed with methanol and activated 10 min at 100°), first with basic mobile phase sec-butanol – pyridine – ammonia – water 39:34:10:26, and (after 12h drying) in the orthogonal direction with acidic mobile phase sec-butanol – pyridine – acetic acid – water 11:8:2:5. Derivatization for peptides and proteins by immersion into fluorescamine (0.05 % in acetone); visualization under UV 254 nm and 365 nm. Computer-assisted determination of the x- and y-coordinates of the derivatized zones. Repeatability (n=8) of the 2D-HPTLC was statistically tested with the Kolmogorov-Smirnov test for normal distribution and with Dixon’s Q test for outliers. Relative standard deviation (RSD) for the RF values was 12.9 % for the first dimension (y-coordinates) and 16.5 % for the second dimension (x-coordinates). According to their higher intensity and sharpness, 15 – 20 detected zones from each protein hydrolyzate were selected, manually scraped from the derivatized layer, dissolved in formic acid solution (0.1 % in acetonitrile – water 3:2), mixed with an equal volume of matrix (dihydroxybenzoic acid 2 % in acetonitrile – water 3:7), crystallized on air on a ground steel target, before being desorbed by the laser beam of the MALDI-TOF-MS/MS (matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry). Direct hyphenation of HPTLC to MS was not performed, to avoid zone diffusion during plate coating with the matrix and to circumvent the stronger binding of polar peptides on the layer.  The MS spectra were acquired in positive reflector mode in m/z range 340 – 4000 (10 – 2500 for fragments), using an external peptide as calibration standard. Identification of 51 from the 85 selected peptides according to AA sequences was performed, using software programs allowing m/z calculation of protein fragments and estimation of cleavage sites. Correlation of the retention behaviour of the peptides with their properties (molecular weight MW, isoelectric point IEP, charges, polarity) was tested with Student’s two-sided t-test after calculation of Pearson’s correlation coefficients. The correlation was significant with IEP, percentages of anionic AA and of non-polar AA; but not with the following properties: MW, percentages of cationic AA and of uncharged polar AA. Finally, based on the correlation results, regression formulas were found to calculate the x- and y-coordinates of any known peptide from the percentage of non-polar AA (or vice-versa). The prediction power of these formulas was verified by repeating the complete 2D-HPTLC-MS experiment with the standard peptides of whey and of peas, and measuring the absolute and relative deviations between the actual x- and y-coordinates and the predicted values. The absolute deviations were higher in the lower RF zones. The average, relative RF value deviations (range 22.1 – 25.7 %) were not different between whey and pea peptides.

      Classification: 2c, 2d, 4e, 18b, 19, 32e
      130 135
      Applications of the solvation parameter model in thin‑layer chromatography
      C. POOLE (Department of Chemistry, Wayne State University, Detroit,
      MI 48202, USA, cfp@chem.wayne.edu)

      J. Planar Chromatogr. 35, 207-227 (2022). The paper discussed the applications of the solvation parameter model in TLC, as a transfer model for biphasic systems in which isotropic interactions in each phase are responsible for the preferential solvation of a solute in one phase over the other. The model emphasizes the importance of water in the mobile phase and the general contributions of mobile phase interactions on the retention mechanism. In addition, the selected solvation of the stationary phase in contact with the mobile phase makes an important contribution to system selectivity. System constants for reversed-phase separations on chemically bonded silica gel HPTLC layers with different mobile phases compositions were also discussed and applied to construct correlation diagrams to facilitate the comparison of selectivity for different layers with the same mobile phase composition or different mobile phase compositions for the same layer.

      Keywords: HPTLC
      Classification: 2c
      130 100
      Hydrophilic retention mechanism of imidazoline and serotonin receptor ligands in thin‑layer and high‑performance liquid chromatography systems
      D. OBRADOVIC*, J. SAVIC, J. JOKSIMOVIC, T. KOWALSKA, D. AGBABA (*Department of Pharmaceutical Chemistry, University, of Belgrade, Faculty of Pharmacy, Belgrade, Serbia, darija@pharmacy.bg.ac.rs)

      J. Planar Chromatogr. 35, 251-263 (2022). HPTLC of ten imidazoline and serotonin
      receptor ligands on amino phase with acetonitrile modified with different volume fractions of methanol and water as mobile phases, acidified with 20 mM ammonium acetate and 0.1 % acetic acid. Detection under UV light at 254 nm. Correlation between retention descriptors and molecular properties was analyzed and different elution orders and separation performances were obtained for the compounds, depending on the chromatographic system employed. 
       

      Classification: 2c
      130 022
      Predicting pharmacokinetic properties of potential anti‑cancer agents using micellar thin‑layer chromatography
      M. JANICKA*, A. SLIWINSKA, M. SZTANKE, K. SZTANKE (*Department of Physical Chemistry, Faculty of Chemistry, Institute of Chemical Science, Maria Curie-Skłodowska University, Lublin, Poland, malgorzata.janicka@mail.umcs.pl)

      J. Planar Chromatogr. 35, 265-272 (2022). HPTLC of newly synthesized azaisocytosine-containing congeners on RP-CN with buffered (pH = 7.4) 0.1 M sodium dodecyl sulphate with 20 % of tetrahydrofuran. Detection under UV light at 254 nm. The micellar chromatographic parameters measured using the TLC technique were proposed as lipophilicity descriptors, and, together with the molecular weight and polarizability, the descriptors were applied in the QSARs methodology to predict the compounds’ blood‒brain distribution. 

      Classification: 2c
      129 020
      Forecasting structure of natural products through color formation process by thin layer chromatography
      L. XU (Xu Lujing), S. LIU (Liu Songbai)* (*Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology, China, songbailiu@zju.edu.cn)

      Food Chem. 334, 127496 (2021). TLC of 36 compounds of 11 classes of bioactives including polyphenols, flavonoids, phytosterols, saponin and saccharides on silica gel. The relationship between structure and color of the bioactives was studied using a broad-spectrum staining solution: p-anisaldehyde - acetic acid - 95 % ethanol - sulfuric acid 920:375:338:125, followed by heating at 250 °C for 10-20 s. Color formation was recorded by a mobile phone camera. Videos were disassembled into frames employing the functions of Matlab. 

      Classification: 2c
      127 015
      Determination of the soil–water partition coefficients (log KOC) of some monoand poly-substituted phenols by reversed-phase thin-layer chromatography
      F. ANDRIC, J. TRIFKOVIC, A. RADOICIC, S. SEGAN, Z. TESIC, D. OPSENIKA* (*Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, P.O. Box 51, 11158 Belgrade, Serbia, dusankam@chem.bg.ac.rs)

      Chemosphere. 81, 299-305 (2010). HPTLC of eleven mono- and poly-substituted phenolic
      compounds on RP-18 with 
      methanol - water (with increasing methanol content from 40-70 %) (1), acetonitrile - water (40-70 %) (2), and acetone - water (45-75 %) (3) and on cyano phase with methanol - water (30-60 %) (4) and acetonitrile - water (30-55 %) (5). In all instances, the content of organic modifier was increased in steps of 5 %. Detection under UV light at 254 nm. Chromatographic descriptors were statistically established to determine the soil–water partition coefficient for the compounds.

      Classification: 2c
      126 033
      A comparative study of chromatographic lipophilicity and bioactivity parameters of selected spirohydantoins
      K. TOT, A. LAZIC, Tatjana SEKULIC* (*Department of Chemistry, Biochemistry and Environmental Protection, University of Novi Sad Faculty of Sciences, Trg Dositeja Obradovica 3, 21000 Novi Sad, Republic of Serbia, tatjana.djakovic-sekulic@dh.uns.ac.rs)

      J. Liq. Chromatogr. Relat. Technol. 43, 925-933 (2020). HPTLC of 21 cycloalkylspiro-5-hydantoins on RP-18 with mixtures of water and organic solvent (acetone: 0.5-0.8 v/v, 1,4-dioxane: 0.5-0.74 v/v, tetrahydrofuran: 0.4-0.7 v/v, acetonitrile: 0.5-0.7 v/v). Detection at UV 254 nm. The logarithm of partition coefficient, log P, for the investigated compounds was calculated. Multivariate data analysis (hierarchical cluster analysis (HCA) and principal component analysis (PCA) was performed.

      Classification: 2c
      126 034
      In silico approach in the assessment of chromatographic parameters as descriptors of diphenylacetamides’ biological/pharmacological profile
      Suzana APOSTOLOV*, D. MIJIN, S. PETROVIC, G. VASTAG (*Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Novi Sad 21000, Serbia, suzana.apostolov@dh.uns.ac.rs)

      J. Liq. Chromatogr. Relat. Technol. 43, 852-862 (2020). HPTLC of diphenylacetamide derivatives on RP-18 with binary mixtures of organic modifier (ethanol, n-propanol, tetrahydrofuran and acetonitrile 36–52 % with 4 % increments). Detection at UV 254 nm. Lipophilicity of examined derivatives was determined. Two multivariate methods, Cluster Analysis, (CA) and Principal Component Analysis, (PCA) were performed to study the compliance of the chromatographic parameters, the standard measure of lipophilicity, the pharmacokinetic predictors and the toxicity parameters of the substances.

      Classification: 2c, 32a