J. Liq. Chromatogr. Relat. Technol. 30, 2253-2265 (2007). HPTLC of 17 flavonoids (caffeic acid, ferulic acid, flavone, naringenin, apigenin, acacetin, luteolin, hesperitin, catechin, epicatechin, hyperoside, hesperidin, quercitrin, narinin, rutin, resveratrol, kaempferol) on silica gel (prewashed with acetone) with 28 binary and ternary mobile phases with chamber saturation. Detection by derivatization with diphenylborinic acid 2-aminoethyl ester (natural products reagent) and evaluation under UV 366 nm. Also coupling of HPTLC and HPLC.

ex O.Berg) for its major anthocyanins by densitometry. J. Planar Chromatogr. 26, 363-369 (2013). HPTLC of delphinidin-3,5-diglucoside (1), petunidin-3,5-diglucoside (2), and malvidin-3,5-diglucoside (3) in the fruits of Eugenia jambolana on silica gel with ethyl acetate - formic acid - acetic acid - water - methanol 126:19:10:32:12. Quantification by absorbance measurement at 529 nm. The hRf values for (1), (2) and (3) were 17, 24 and 34, respectively. Linearity was in the range of 400-1000 ng/zone for (1) and (2) and 200-500 ng/zone for (3). LOD and LOQ were 84 and 283 ng/zone for (1), 72 and 242 ng/zone for (2) and 43 and 154 ng/zone for (3), respectively. Average recoveries were 95.4 % for (1), 96.3 % for (2) and 97.3 % for (3). Intermediate/interday/intra-day precision was below 5 % (n=3).

Food Chem. 187, 460-468 (2015). HPTLC-direct bioautography of bioactive compounds in the extracts of cold-pressed hemp (1), flax (2) and canola (3) seed oil on silica gel with toluene - ethyl acetate - formic acid - water 15:30:5:3 for (2) and toluene - ethyl acetate - acetic acid 80:25:4 for (1) and (3). HPTLC-DPPH scavenging activity was determined by dipping into a methanolic DPPH solution, followed by drying for 90 s in the dark and heating at 60 °C for 30 s. The hRF values of dominant radical scavenging zones were in the range of 75-85 for (1), 70-90 for (2) and 64 and 95-100 or (3). HPTLC-antimicrobial Aliivibrio fischeri assay allowed the determination of major antimicrobial zones at hRF 40-49 and 55-66 or (1), 23, 45 and 60 for (3) and 95 for (2). Additional effect-directed analyses employing acetylcholinesterase (AChE) assay, planar yeast estrogen (pYES) bioassay and Bacillus subtilis bioassay as well as subsequent HPTLC-ESI-MS allowed targeted characterization of bioactive compounds.

J. Food Comp. Anal. 48, 25-33 (2016). HPTLC of kavalactones (K, DHM, DHK, M, DMY, Y) and flavokavins (FKA, FKB, FKC) in kava (Piper methysticum) on silica gel with hexane – dioxane 4:1. Individual standards of the following nine compounds of interest were applied on plates and scanned after elution to obtain their UV absorption maxima: flavokavin A (FKA, 361 nm), flavokavin B (FKB, 343 nm), flavokavin C (FKC, 368 nm), kavain (K, 247 nm), dihydromethysticin (DHM, 200 nm), dihydrokavain (DHK, 242 nm), methysticin (M, 306 nm), demethoxyyangonin (DMY, 338 nm), and yangonin (Y, 354 nm). Detection by dipping for 1 s into anisaldehyde – sulfuric acid reagent (10 mL sulfuric acid with 170 mL of ice-cooled methanol, 20 mL of acetic acid, and 1 mL of anisaldehyde reagent), followed by heating at 100 ºC for 3 min. Quality of the samples was assessed by computing two ratios after scanning the plates at 245 nm (kavain/total kavalactones; K/KL) and 366 nm (flavokavins/kavalactones; FK/KL). The ratio K/KL corresponds to the peak area of K versus the sum of the peak areas of all other kavalactones (DHM + DHK + M + DMY + Y). The ratio FK/KL corresponds to the sum of the peak areas of the three FK (A + B + C) versus the peak areas of Y and DMY._x000D_

J. Ethnopharmacol. 193, 490-499 (2016). HPTLC of quercetin in the leaves of Ocimum basilicum on silica gel with toluene – ethyl acetate – formic acid 17:11:2. Quantitative determination by absorbance measurement at 254 nm.

J. Chromatogr. A 1530, 192-196 (2017). HPTLC to measure the antioxidant and hypoglycemic effects in different extracts from Myrmecodia platytyrea, which can be a traditional medicine as alternative treatment for diabetes, because the substances produced by ants can reduce blood sugar levels. Measurement of the antioxidant activity in methanol, ethanol, dichloromethane (DCM) and ethyl acetate (EA) extracts with the HPTLC-2,2-diphenyl-1-picrylhydrazyl free radical (DPPH*) assay, and of the hypoglycemic effects with a newly developed α-amylase inhibitory activity assay. Detection of stigmasterol after derivatization with anisaldehyde reagent as purple colored zone under white light at hRF 66. The results showed the highest antioxidant activity in the ethanol extract rich in polyphenols and flavonoids. No antioxidant activity but significant α-amylase inhibitory activity was observed in the DCM extract. The highest α-amylase inhibitory activity was found in the EA and DCM extracts and was related to their stigmasterol content.

ex Schult. Pharmacogn. Mag. 14, 630-637 (2018). HPTLC of quercetin (1), kaempferol (2) and myricetin (3) in the aerial parts and roots of Aerva lanata on silica gel with toluene – ethyl acetate – formic acid 12:8:1. Quantitative determination by absorbance measurement at 254 nm. Linearity ranged between 100-1000 ng/zone for (1) and 25-1250 ng/zone for (2) and (3).

Biochem. System. and Ecology 12, 183-188 (1984). TLC on silica with ethyl acetate - isopropanol - water 65:22:11; visualization by spraying with 85 % phosphoric acid - acetic acid - aniline - diphenylamine 20 ml + 100 ml + 5 ml + 5 g and heating at 100 °C for 2-3 h. Identification of sugars by co-chromatography with authentic standards.