(Des plantes pour maigrir: dangers). Schweiz. Apothekenzeitung 139, 82-85 (2001). TLC of aristolochic acid and methanolic plant extracts from the Chinese plant Aristolochia fangchi on silica gel with chloroform - methanol - acetic acid 65:20:2. Detection 1) by spraying with 0.5% diphenylamine in 60% sulfuric acid and heating for 10 min at 100°C; 2) observation under UV at 366 nm. Simple and fast TLC method.

J. Liq. Chrom. & Rel. Technol. 25, 1623-1632 (2002). Quantitative silver ion TLC and RP-TLC were applied in complementary ways to determine the triacylglycerol composition of sesame seeds. Preparative isolation of TAGs on silica gel with petroleum ether - acetone 25:2. Detection by spraying the edge of the plate with 2,7-dichlorofluorescein. TLC of TAGs on silica gel impregnated by dipping into a 0.5% methanolic solution of silver nitrate, with petroleum ether - acetone 25:1 and hexane - acetone - ethanol 25:2:1. Detection by treatment with bromine and sulfuryl chloride vapors and charring at 180-200°C. Quantitative RP-TLC on silica gel treated for 6 hours with vapors of DMDS and washed with methanol. A three component mobile phase, acetone - acetonitrile - water 7:3:x was used with acetone - acetonitrile ratio kept constant and varying the water proportions. Visualization by spraying with 50% ethanolic sulfuric acid and heating at 200-220°C. Quantitation by densitometry at 450 nm.

Anal. Chim. Acta 488, 203-209 (2003). Dansylation of aromatic carboxyl compounds (i.e. aspirin), aromatic primary amines, and aliphatic carboxyl compounds in 1 M Na2CO3 buffer at pH 11. TLC on silica gel of fluorescent labeled analytes using methylene chloride, ethyl acetate, acetone, or desired mixture of the solvents. Methylene chloride was superior to ethyl acetate or acetone. Fluorescent analytes were observed under UV lamp. Limits of detection for dansylated carboxyl compounds was 1-5 µg.

Part IV. Separation on RP 18 plates with ternary mobile phases. J. Planar Chromatogr. 18, 228-233 (2005). HPTLC of heptanoic to eicosanoic acids on RP-18 (with and without concentrating zone). The best chromatographic conditions for separation of the fatty acids were RP-18 plates without concentrating zone and methanol - ethanol - water 9:9:2, and RP 18 plates with concentrating zone and methanol - ethanol - water or methanol -n-propanol - water 9:9:2. Detection by exposure to iodine vapour. Separation of acids from methanoic to butanoic and from tetracosanoic to triacontanoic acid was not possible.

J. Planar Chromatogr. 20, 141-143 (2007). TLC of methyl, ethyl, propyl p-hydroxybenzoate, p-hydroxybenzoic acid, benzoic acid, sodium benzoate, butylated hydroxyanisol, and butylated hydroxytoluene on the inorganic ion exchanger stannic silicate in a twin-trough chamber with n-hexane - ethyl methyl ketone - acetic acid 80:20:3. Quantitation by scanning densitometry at 260 nm.

60th Indian Pharmaceutical Congress PA-217 (2008). HPTLC of oleanolic acid in total methanolic extract of fruits of Randia dumetorum lam. on silica gel with toluene - ethyl acetate - acetic acid 70:30:1 in a twin trough chamber saturated for 10 min. Detection by treatment with 10 % sulphuric acid in methanol, followed by heating at 110 °C and immediate densitometric evaluation. Quantitative determination by absorbance measurement at 540 nm. The method was linear in the range of 50-500 ng/spot. Recovery was in the range of 99.4-100.8 %.

International Journal of PharmaTech Research 03, 527-538 (2009). A stability indicating HPTLC method has been developed for analysis of cephalexin in bulk and dosage formulation. HPTLC on silica gel with ethyl acetate - methanol - 25 % ammonia 6:4:1. The hRf value was 56. Densitometric quantification at 260 nm. The method was linear in range of 500-1500 ng/band. Cephalexin was subjected to forced degradation (acid, alkali, oxidation, thermal, photolytic). All degradation products were well separated from the drug, indicating specificity of the method.

Scholars Research Library 2(1), 489-494 (2010). HPTLC of montelukast sodium and levocetirizine dihydrochloride on silica gel with toluene - chloroform - methanol - glacial acetic acid 6:20:10:1 in a twin trough chamber with chamber saturation for 25 min. The hRf value of levocetirizine was 64 and of montelukast 89. Densitometric evaluation at 302 nm. The method was linear in the range of 200-3200 ng/band for montelukast and 400-1200 ng/band for levocetirizine. The recovery was 99.9-100.0 %.