Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      131 084
      Application of the green analytical procedure index to the simultaneous analysis of co formulated tinidazole and ciprofloxacin using an environmentally friendly micellar HPTLC technology
      R. SARAYA*, Y. HASSAN, W. ELTOUKHI, B. SALMAN (*Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Port Said University, Port Said 42511, Egypt,

      J. Planar Chromatogr. 36, 21-30 (2023). HPTLC of tinidazole (1) and ciprofloxacin (2) in pure form, tablet dosage form and human plasma on silica gel with acetone - ethanol - 2 % watery sodium dodecyl sulfate 3:4:2. Quantitative determination by absorbance measurement at 310 nm. The hRF values for (1) and (2) were 22 and 42, respectively. Linearity was in the range of 25-1000 ng/zone for (1) and 80-1000 ng/zone for (2). Intermediate precisions were below 2 % (n=3). LOD and LOQ were 7 and 20 ng/zone for (1) and 25 and 75 ng/zone for (2). Average recovery was 99.9 % for (1) and 99.7 % for (2).

      Classification: 32c
      131 040
      Analytical approaches for determination of COVID-19 candidate drugs in human biological matrices
      R. MAHDAVI, Z. TALEPOUR (*Department of Analytical Chemistry, Faculty of Chemistry, Alzahra University, Vanak, Tehran, Iran,

      Trends Anal. Chem. 160, 116964 (2023). Review of the analysis techniques for the diagnosis and determination of candidate drugs in the treatment of COVID-19 in human biological fluids in the period 2015-2022, including TLC and HPTLC. Sofosbuvir, daclatasvir, ledipasvir, and ribavirin are some of the common antiviral drugs for the treatment of COVID-19, whose separation and simultaneous analysis was performed by HPTLC.

      Classification: 1b, 32a
      131 055
      Analytical method development for exploring pharmacokinetic profile of ursolic acid in rat tissues by high‑performance thin‑layer chromatography
      P. BHATTACHARYA, A. SAHA, S. BASAK* (*Department of Chemical Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 700009, India,

      J. Planar Chromatogr. 36, 9-19 (2023). HPTLC of ursolic acid in rat tissues on silica gel and amino phase with toluene - ethyl acetate - methanol 4:1:1. Detection by heating at 120 °C for 3 min, followed by dipping into 5 % liquid paraffin in hexane. Quantitative determination in remission/fluorescence mode at 366 nm. The hRF value for ursolic acid was 60 in normal phase and 50 in amino phase. Linearity was in the range of 96-384 ng/zone. Recovery was between 94.7 and 97.7 %. 

      Classification: 14
      130 147
      Globotriaosylceramide-related biomarkers of Fabry disease identified in plasma by high-performance thin-layer chromatography – densitometry – mass spectrometry
      C. JARNE, L. MEMBRADO, M. SAVIRÓN, J. VELA, J. ORDUNA, R. GARRIGA, J. GALBÁN, V. L. CEBOLLA* (*Institute of Carbon Chemistry, Spanish National Research Council (CSIC), Saragossa, Spain;

      J Chromatogr A 1638, 461895 (2021). Samples were sphingolipid-rich fractions of unproteinated blood plasma from healthy humans or from Fabry’s disease patients, as well as standards of sphingomyelin (SM) and of globotriaosylceramides (Gb3 = ceramide trihexosides), and related compounds (lyso-ceramide trihexosides, lactosyl ceramide, glucosyl ceramide). HPTLC on silica gel (Lichrosphere with spherical particles) by automated multiple development with a 9-step gradient, starting with pure methanol and ending with dichloromethane – methanol 9:1. Visualization and densitometry under UV 190 nm. Derivatization for Gb3 and derivatives (but not for SM) by immersion into orcinol solution (0.2 %, with sulfuric acid 10 %), followed by 15 min heating at 100 °C and by densitometry under visible light 550 nm. Bands of interest were directly eluted with methanol from underivatized plates into an ion-trap MS, through the oval head of a TLC-MS interface (with stainless steel frit to remove silica gel particles). Two different ionization processes were used: (A) electrospray ionization (ESI, capillary voltage 4 kV, endplate offset voltage -0.5 kV, nebulizer pressure 40 psi, drying gas 9 mL/min at 350 °C); (B) atmospheric pressure chemical ionization (APCI, capillary voltage 2–3 kV, current intensity 4.5 µA, nebulizer pressure 45 psi, drying gas 5 mL/min at 350 °C; vaporization at 450 °C). Full MS spectra were recorded up to m/z 1500 in positive ion mode. The relative ion intensities were used to quantify the detected species. Previous to this study, the precision of the elution head positioning was tested on Gb3 standard zones, comparing 3 positions for analyte elution: from the centre and from each higher or lower side of the band. The same main m/z peaks were observed in the 3 positions, but in different proportions. This was explained by the presence of coeluting Gb3 subclasses (the ceramide moiety CM being either saturated, mono-unsaturated fatty acyl with a slightly higher migration distance, or polar hydroxyl fatty acyl with the opposite effect on migration) and of coeluting Gb3 isoforms (the hexoside moiety consisting of glucose and/or galactose units). This resulted in the broadening and partial splitting of the standard band. In the plasma samples, 19 molecular species of Gb3 were identified (depending on the CM, the sugar isoforms being undistinguishable by MS): 5 with a saturated CM, 7 with two additional double bonds on the CM, 7 with a methylated CM. In case of Fabry’s disease, most Gb3 species with saturated CM were highly increased, whereas other species were decreased.

      Classification: 4e, 11c, 11e, 32f
      130 057
      Sensitive and selective bioscreening of the most commonly used coronavirus disease drug, favipiravir, and its co-administered therapeutic, meropenem, in human plasma
      R. ABDELFATAH, E. ABDELALEEM, E. ABDELMOMEN*, R. ABDELMOETY, A. EMAM (*Faculty of Pharmacy, Nahda University (NUB), New Beni-Suef City, Egypt,

      J. Sep. Sci. 45, 3800-3810 (2022). HPTLC of favipiravir (1) and meropenem (2) in human plasma on silica gel with ethyl acetate - methanol - water - formic acid 50:40:15:3. Quantitative determination by absorbance measurement at 300 nm for (1) and (2). The hRF values for (1) and (2) were 34 and 10, respectively. Linearity was between 0.1 and 20 µg/mL for (1) and 10 and 60 µg/mL for (2). Inter-day and intra-day precisions were below 5 % (n=3). The LOQ were 0.1 and 10 µg/mL for (1) and (2), respectively. Mean recovery was 99.7 % for (1) and 98.0 % for (2).

      Classification: 32f
      130 005
      Multiobjective optimization of microemulsion – thin layer chromatography with image processing as analytical platform for determination of drugs in plasma using desirability functions
      Noura H. ABOU-TALEB*, D. T. EL-SHERBINY, N. M. EL-ENANY, H. I. EL-SUBBAGH (*Medicinal Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt;

      J Chromatogr A, 1619, 460945 (2020). Samples were lamotrigin as standard, or extracted with an oil-in-water microemulsion (10 µL butyl acetate, 4 mL n-butanol, 925 mg sodium dodecyl sulphate, 8.6 mL water) either from patients’ raw plasma (for separation from blood proteins) after spiking, or from commercial tablets dissolved in methanol. TLC on silica gel with a water-in-oil microemulsion of 9 mL butyl acetate, 1 mL n-butanol, 250 mg sodium dodecyl sulphate, 250 µL water. Both optimal microemulsions were predicted using Taguchi orthogonal array and Plackett-Burman design. Evaluation in UV 254 nm, quantification from the digital picture using four image processing software programs. For lamotrigin (hRF 24), limits of quantification were 170 ng for pure drug and 10 ng for spiked plasma. Linearity (in range 20–200 ng/spot) was directly obtained for the calibration curve in spiked plasma; however, for pure drug, linearity was obtained only when using log values of the calculated densities (300–3000 ng/spot).

      Classification: 3a, 3d, 5c, 23e, 32c
      130 012
      HPTLC method for the ultrasensitive detection of triamterene in plasma
      A.A. KHORSHED, M.M. ELSUTOHY*, A.A. MOHAMED, M. ORABY (*2500 Univ. Drive, Schulich Sch. of Engin., Univ. of Calgary, Calgary, Alberta T2N 1N4, Canada,

      J. Chromatogr. Sci. 60 (3), 267-273 (2022). HPTLC for the selective detection of the diuretic drug triamterene in pure form, tablets and human plasma, on silica gel with ethyl acetate - dimethylformamide - ammonia 70:27:3. Quantitative determination by fluorescence measurement at 440 nm improved the method sensitivity 250-fold compared with previously reported studies, and enabled the detection of triamterene in the linear concentration range of 0.8 to 60 ng/band for the pure drug and 1.0 to 60 ng/band for biological samples (human plasma).

      Classification: 32a
      128 004
      Validation and application of a protocol for the extraction and quantitative analysis of sphingomyelin in erythrocyte membranes of patients with non‑alcoholic fatty liver disease
      C. PAPADOPOULOS, K. MIMIDIS, I. TENTES, T. TENTE, K. ANAGNOSTOPOULOS* (*Laboratory of Biochemistry, Department of Medicine, Faculty of Health Sciences, Democritus University of Thrace, 68100 Alexandroupolis, Greece,;

      J. Planar Chromatogr. 34, 411-418 (2021). HPTLC of sphingomyelin in erythrocyte membranes of patients on silica gel with chloroform - methanol - acetic acid - water 60:50:1:4. Detection by exposure to iodine vapor for 30 min. Quantitative determination by densitometric measurement of the intensity of individual zones and the red, green and blue values of the component colors. The hRF value for sphingomyelin was 86. Linearity was between 0.25 and 10 μg/zone. LOD and LOQ were 140 and 410 ng/zone, respectively. Intermediate precisions were below 2 % (n=3). Recovery was between 85 and 97 %.

      Classification: 11c