J. of China Pharm. 23 (20), 38-40 (2014). Nicotine is a toxic substance in tobacco. Studies have shown that nicotine may prevent and treat Alzheimer's disease (AD) and Parkinson's syndrome (PD). At present, the most effective method to treat AD is by inhibiting the activity of acetylcholinesterase (AChE), thus enhancing the cholinergic activity indirectly. In order to clarify the mechanism of nicotine reducing the incidence of AD, the activity of natural AChE inhibitors in Xinjiang Mohe tobacco was screened by TLC on silica gel with (A) ethyl acetate – methanol – ammonium hydroxide 70:30:1, followed by detection via enzyme inhibition (this is not bioautography, as stated in the title): firstly by spraying with 1.0 U/mL AChE (AChE 500 U + tris-HCl buffer solution of pH 7.8 500 mL + bovine serum albumin 500 mg), followed with 1.5 g/L alpha-naphthyl acetate (150 mg in ethanol 40 mL + water 60 mL) and then with 0.5 g/L Fast blue B salt, resulting in white zones on purple background; (B) toluene – ethyl acetate - diethylamine 7:2:1, detection by spraying with 0.2 mmol/L 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical reagent, resulting in white to yellow zones on pale purple background. The results indicated that nicotine in Xinjiang Mohe tobacco could bind to AChE and thus inhibit its activity.

(Fluorodensitometric determination of Michaeladducts of (alpha, beta unsaturated aldehydes in biological material.) Arzneim. Forsch. 35, 552-554 (1984). TLC of dansylhydrazine derivatives of Michael adducts of alpha, beta-unsaturated aldehydes on silica with isopropanol - methyl acetate - methanol - NH3 50:5:30:20, resp. chloroform - petrol ether - acetone 7:1:2. Detection after drying and dipping into 20 % polyethyleneglycol 400 in chloroform by UV. Quantification by fluorescence scanning. Detection limit 10 ng.

J. Chromatogr. 374, 221-222 (1986). TLC of 6 estrogen conjugates on C-8 silica with methanol - tetramethylammonium chloride 1:1. Detection by spraying with 10 % sulfuric acid and heating at 120 °C for 5 mm. Quantification by densitometry at 336 nm. Sensitivity 50 ng, calibration curves linear at 50 -2000 ng.

J. Chromatogr. 382, 308-313 (1986). TLC of 5 phospholipids, 5 neutral lipids, 4 cholesterol ester subfractions on silica with 2 successive runs with chloroform - methanol - water 65:25:4 and n-hexane - acetone 100:1. Analysis of lipids in human blood.

(Chinese). Chinese J. Adv.in Biochem.& Biophys. 2, 37-40 (1987) (Shengwu Huaxue Yu Shengwu Wuli Jinzhan). TLC of 6 amino acids on polyamide with a) benzene - acetic acid 9:1, b) 1.3% aqueous formic acid, c) ethyl acetate - methanol - acetic acid 20:1:1. Detection by dansylation. Quantification by fluorometry.

J. of Natural Products 51, 532-536 (1988). TLC of papaverine and papaveraldine on silica with chloroform - acetone 70:30 or n-butanol saturated with water - methanol 90:10. Detection under UV.

J. of Medicinal Chemistry 32, 174-179 (1989). TLC on silica with 1-butanol - acetic acid - pyridine - water 5:5:4:1, 2-propanol - 25% NH3 - water 3:1:1, ethyl acetate - pyridine - acetic acid - 5:5:1:3 and butanol - acetic acid - water 4:1:5. Visualization with ninhydrin, fluorescamine and iodine vapor.

J. Chromatogr. 488, 5-24 (1989). Consideration of various facets of the history of clinical chromatography with emphasis on their application to the present state of the art, including Lato’s TLC studies of sugars in urine and other chromatographic techniques.