J. Chromatogr. B 823 (2), 152-157 (2005). HPTLC of lamotrigine (extracted from serum by ethyl acetate) on silica with toluene - acetone - ammonia 14:6:1. Densitometric measurement at 312 nm, hRf of lamotrigine at 54. The analytical method has excellent linearity (r = 0.998) in the range of 20–300 ng/spot. This assay range is adequate for analyzing human serum, as it corresponds to lamotrigine concentrations measured in human serum from epileptic patients. The method was validated for sensitivity, selectivity, extraction efficiency, accuracy and intra and inter-day reproducibility. The limit of detection and limit of quantification were found to be 6.4 and 10.2 ng, respectively. Good accuracy and high precision (CV) is reported, i.e. in the range of 92.1-97.1 % and 0.5-2.6 % respectively. The method was applied for determination of serum lamotrigine levels in epileptic patients and in pharmacokinetic study of lamotrigine administered orally to rabbits.

CBS 101, 2-4 (2008). HPTLC of amino acids (from hydrolysis of peptides) on silica gel, Diol phase, and cellulose with either 2-butanol - acetic acid - pyridine - water 15:3:10:12 or 2-butanol - 25 % ammonia - pyridine - water 39:10:34:26 in a twin-trough chamber or horizontal chamber. Detection by dipping in ninhydrin solution (0.5 % in 2-propanol) followed by heating at 110 °C for 5 min. Better results are achieved by adding ninhydrin directly to the mobile phase at a 0.5 %-level. Quantitative determination by absorbance measurement at 440 nm. Selectivity was better on the cellulose and silica gel plate. Selection of the chromatographic system depended on which amino acids had to be separated and no general recommendation could be given. HPTLC analysis of a hydrolyzed peptide sample (containing Phe, Trp, D-Bal, Apc and Inp) on cellulose with the acidic mobile phase. All five amino acids were quantified between 70 and 130 % of the theoretical value for non-stable amino acids (degradation 5 to 30 %).

Asian Journal of Plant Sciences 9(1), 44-50 (2010). Beta-sitosterol is one of the major phytoconstituents in cornsilk (style and stigma of Zea mays Linn.). An HPTLC method for the standardization of cornsilk as a bioavailable source of beta-sitosterol is reported. HPTLC of methanolic extracts of dried cornsilk and plasma of rabbits (1 h after injection of plant slurry) on silica gel with toluene - ethyl acetate - methanol - glacial acetic acid 80:10:5:3 with chamber saturation for 30 min. Detection by treatment with Liebermann-Burchard reagent. Beta-sitosterol was well resolved with an hRf value of 48. Quantitative determination by fluorescence measurement at 366 nm. The method was suitable for the pharmacokinetic study (absorption - elimination). The study confirmed the bioavailability of beta-sitosterol from cornsilk, which is therefore a potential natural source of beta-sitosterol.

J. Planar Chromatogr. 24, 428-434 (2011). HPTLC of 6-benzyl-, 6-methyl-, and 6-propyl-2-thiouracil and spiked urine samples on silica gel with methanol in a horizontal chamber saturated for 15 min at ambient temperature. Detection by spraying with a freshly prepared mixture of 4 % sodium azide and 1 % starch solution adjusted to pH 5.5, followed by exposure to iodine vapor for 5 s. Quantitative evaluation by use of an office scanner at 300 dpi resolution. The images were inverted and stored in the form of 24-bit-true color images, which were analysed by TLSee software. The determination range was 7-16 pmol/zone, 80-160 nmol/mL urine, or 133-266 nmol/mL serum. The recovery was between 93-106 %. The LOQ was 4 pmol/zone for the studied thiouracils in three investigated matrices.

J. Planar Chromatogr. 28, 152-156 (2015). HPTLC of cholesterol from lung tissues on silica gel with petroleum ether - diethyl ether - acetic acid 90:10:1 in an unsaturated chamber. Detection by dipping in phosphomolybdic acid. Quantitative determination by absorbance measurement at 580 nm. Comparable results were obtained by HPLC analyses of the same samples.

Lipids 17, 107-110 (1982). TLC of glycolipids (glucocerebromide, lactosyl ceramide, globotriosyl ceramide, gangliotriosyl ceramide, globoside I, asialo-GM1) on silica with chloroform methanol - 0.2 % aq. CaCl2 55:45:10. Detection with anthrone-sulfuric acid reagent.

Apoth. Ztg. 125, 2062-2068 (1985). Dtabilität von Barbitursäurederivaten in wässrigen Lösunge. (Stability of barbiturates in aqueous solutions.) TLC on silica with isopropanol - chloroform -23 % NH3 45:45:10. Phenylacetylurea was found to be the main product of hydrolytic degradation of phenobarbital. Stability testing of solutions of aminobarbital, barbital and phenobarbital in dependence of the pH-value showed minor degradation rate between pH 8.0 and 9.5 than at higher pH-values.

Detection under UV at 365 nm. Quantification by fluorometry following elution with methanol.