Biomed. Chromatogr. 10, 245-250 (1996). Description of methods for glycosphingolipid extraction in excellent yield without the need for using toxic chloroform. TLC of lipid extracts on silica with various solvent systems. Detection by spraying with different reagents. Quantification by densitometry at 550 nm and 650 nm. Determination of sialic acid by HPLC. Calculation of the rank correlation coefficient. Comparison of the alternative solvent mixtures and chloroform in yields.

CBS 93, 2-4 (2004). HPTLC of signaling ceramides on silica gel with a 7 step gradient from methanol over dichloromethane to n-hexane over 42 min. Detection by dipping in manganese chloride reagent for 1 s, followed by drying at 120 °C for 20 min. Quantitative determination by absorbance measurement at 550 nm and Michaelis-Menten regression 2 via peak area. Signaling ceramides are separated from other lipids (shingomyelin, phosphatidylcholine, cholesterol) contained in cellular lipid extracts. Comparison with determination of ceramide formation via isotope labeled standards and conventional TLC method.

J. Liq. Chromatogr. Relat. Technol. 28, 2571-2580 (2005). TLC of lipids, triacylglycerol, diacylglycerol, and sphingosine on silica gel with hexane - diethyl ether - formic acid 40:10:1 for neutral lipids, and with chloroform - methanol - water 65:25:4 for glycolipids. Phospholipids were separated by two dimensional development with chloroform - methanol - 28 % ammonium hydroxide 65:25:4 in the first direction, followed by drying and development with chloroform - acetone - methanol - acetic acid - water 30:40:10:10:1 in the second direction. Detection of neutral lipids by spraying with charring or phosphomolybdic acid reagent; detection of glycolipids by spraying with orcinol reagent (orcinol in 70 % sulfuric acid), and of phospholipids by spraying with molybdenum blue reagent for phosphate groups or ninhydrin reagent for phospholipids containing free amino groups.