Chinese J. Chromatogr. (Sepu) 12, 380-381 (1994). TLC of lactic acid dehydrogenase (LDH) and LDH isozyme on polyacrylamide with Tris-Gly system (0.05 mol/L, pH 7.9). Detection by densitometry. Investigation of the periodic variation by comparison of the finger prints of the samples sampled during hedgehog hibernation.

J. Chromatogr. 698, 361-367 (1995). Two-dimensional gel electrophoresis of proteins. Discussion of the approach in providing data on protein synthesis in different stages of limb regeneration or comparative data with other regenerative processes such tail and lens regeneration. Comparison with other methods employing gene cloning or generation of antibodies.

Biomed. Chromatogr. 10, 251-255 (1996). Gel electrophoresis on 15% polyacrylamide gel at pH 8.3 and pH 4.5. Detection by staining for 1 h in a 12.5% trichloracetic acid, development with 0.25% Coomassie Blue in 35% methanol for 1 h, and destained in 10% acetic acid. Quantification by densitometry. Purification by preparative chromatography on Amberlite XAD-7-gradient elution.

Abstract G-33, IPC (2005). HPTLC of epalrestat in plasma on silica gel with ethyl acetate - toluene - acetic acid 30:20:1. Nitrofuranloin was used as internal standard. Quantitative determination by absorbance measurement at 390 nm. Linearity was obtained the range of 0.01-0.20 µg/mL with recovery of 99-107 %. The method was validated as per ICH guidelines.

J. AOAC Int. 91, 1218-1226 (2008). HPTLC of glycosphingolipids on silica gel with chloroform - methanol - water 60:35:8 or 65:25:4. Detection by spraying with orcinol reagent followed by heating at 110 °C. Detection with HPTLC/MS by direct coupling of HPTLC to matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry showed to be a reliable and reproducible method to obtain structural information and fundamental properties of glycosphingolipids.

IPA Convention, 2010, RA-PO 03. HPTLC of edaravone in samples of human plasma (purified by liquid-liquid extraction) on RP-18 with n-butanol - methanol - diethyl ether 1:8:1. The compound was well resolved with an hRf value of 81. Densitometric evaluation at 240 nm. The limit of detection and quantification was 25 ng and 150 ng respectively. The linearity was 600-2400 ng/band. Recovery (%) based on analysis of spiked sample was more than 65 %.

J. Ethnopharmacol. 137, 99-104 (2011). HPTLC of karanjin in rabbit plasma on silica gel with dichloromethane - toluene - methanol 35:15:3. Qualitative evaluation under UV 366 nm and quantitative determination by densitometry at 366 nm. Linearity was between 1.0 and 15.0 µg/mL. LOD and LOQ were 0.5 and 1.0 µg/mL, respectively. The inter-day and intra-day accuracies were 97.1 % and 92.2 %, respectively. Recovery (by standard addition) was between 98.2 % and 99.9 %.

J. Planar Chromatogr. 27, 416-419 (2014). HPTLC of (1) cotinine and (2) trans-3'-hydroxycotinine in urine on RP-18 with acetonitrile - water 4:1. Quantitative determination by absorbance measurement at 260 nm. Linearity was in the range of 65-325 ng/zone (at a low calibration range) and 325-1300 ng/zone (at a high calibration range) for (1) and 62.5-312.5 and 312.5-1250 ng/zone for (2). The intermediate precisions were below 9 % (n=3). The LOD and LOQ were 13 and 38 ng/zone for (1) and 17 and 50 ng/zone for (2), respectively.