Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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      129 015
      Comparison of yeast estrogen screening on HPTLC and in microtiter plates
      A. BERGMANN*, E. SIMON, A. SCHIFFERLI, A. SCHÖNBORN, E. VERMEIRSSEN (*Swiss Centre for Applied Ecotoxicology, Eawag, Überlandstrasse 133, 8600 Dübendorf, Switzerland, alanjames.bergmann@oekotoxzentrum.ch)

      CBS 125. HPTLC of estrogen-active compounds in a migrate of a food contact material on silica gel with chloroform - acetone - petroleum ether 11:4:5. Planar Yeast Estrogen Test (P-YES) was performed by spraying 2 mL yeast cells onto HPTLC plates, followed by incubation at 30 °C for 3 h and spraying with 2 mL 0.5 mg/mL 4-methylumbelliferyl-beta-D-galactopyranoside and incubation for 20 min at 37 °C. The method was compared with microtiter bioassay (L-YES). P-YES was more sensitive than L-YES and results can be repeated upto one year later.

      Classification: 4e, 13b
      129 029
      New solvent systems to separate some estrogenically active compounds by high‑performance thin‑layer chromatography (HPTLC)
      B. SPANGENBERG (Institute of Process Engineering, Offenburg University of Applied Sciences: Hochschule Offenburg, Badstrasse 24, 77652 Offenburg, Germany, Spangenberg@HS-Offenburg.de)

      J. Planar Chromatogr. 35, 189-195 (2022). Planar yeast estrogen screen (pYES) of estriol, daidzein, genistein, 17β-estradiol, 17α-ethinyl estradiol, estrone, 4-nonylphenol and bis(2-ethylhexyl) phthalate on silica gel with cyclohexane - methyl ethyl ketone 2:1 or cyclohexane - cyclopentyl-methyl ether 3:2. Detection under UV light at 272 nm.

      Classification: 13b
      127 030
      High-performance thin-layer chromatography in combination with a yeast-based multi-effect bioassay to determine endocrine effects in environmental samples
      N. BAETZ, L. ROTCHE, V. WIRZBERGER, B. SURES, T. SCHMIDT, J. TUERK* (*Institute of Energy and Environmental Technology, Bliersheimer Str. 58 – 60, 47229 Duisburg, Germany, tuerk@iuta.de)

      Anal. Bioanal. Chem. 413, 1321-1335 (2021). HPTLC of estrone (1), 17β-estradiol
      (2), 17α-ethinylestradiol (3), 5α-dihydrotestosterone (4), and progesterone (5) in wastewater and surface water samples on silica gel with a 
      mixture of dichloromethane, cyclohexane, and acetone in different proportions. Detection by spraying with 8 % sulfuric acid in ethanol, followed by heating at 105 ºC for 10 min. Qualitative identification under UV light at 310 nm. Yeast multi endocrine-effect screen was performed by spraying the HPTLC plates with a mixed suspension of genetically modified Arxula adeninivorans yeast strains, which contain either the human estrogen, androgen, or progesterone receptor. The HPTLC plates were incubated at 30 ºC for 18 h and at 100 % humidity. After incubation, densitometric evaluation at: 445/K460 nm, 475/K500 nm and 542/K560 nm to determine the fluorescence of the cyan fluorescent protein (CFP, gestagen), green fluorescent protein (GFP, androgen), and DsRed2 protein (estrogen), respectively. The hRF values for (1) to (5) were 21, 22, 29, 34 and 39, respectively.

       

       

      Classification: 13b
      123 009
      Equol determination in cattle manure by HPTLC-DART-TOF-MS
      V. PETERS, B. SPANGENBERG* (*Department of Process Engineering, University of Offenburg, Badstrasse 24, 77652 Offenburg, Germany, Spangenberg@HS-Offenburg.de)

      J. Liq. Chromatogr. Relat. Technol. 42, 311-316 (2019). HPTLC of equol in cattle manure with methyl t-butyl ether - cyclohexane 1:1. The plate was scanned with a Time of Flight – Direct Analysis in Real Time – Mass Spectrometry (TOF-DART-MS) system. The hRF value of equol was 71. The LOD and LOQ for equol were 2.4 µg/zone and 4.5 µg/zone, respectively. 

      Classification: 13b
      120 086
      Bioprofiling of Salicaceae bud extracts through high-performance thin-layer chromatography hyphenated to biochemical, microbiological and chemical detections
      S. HAGE, Gertrud E. MORLOCK* (*Chair of Food Sci., Inst. for Nutr. Sci. & Interdisciplinary Res. Center (IFZ), Justus Liebig Univ. Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany, Gertrud.Morlock@uni-giessen.de)

      J. Chromatogr. A 1490, 201-211 (2017). HPTLC for comparison of the phenolic profiles of polar extracts from Populus nigra L. (1), Populus alba L. (2) and Salix alba L. (3) buds. Five chemotypical patterns were distinguished after derivatization with Natural Products reagent and confirmed by principal component analysis. The HPTLC analysis was directly hyphenated to various microbiological and biochemical assays as well as spectrometric techniques, which directly linked to active molecules in the chromatograms. The results showed that polyvalent compounds were evident when all derivatization and activity assays were combined together. Detection of at least three antimicrobial compound zones using Aliivibrio fischeri and Bacillus subtilis bioassays and of one phyto-estrogen with the planar yeast estrogen screen in Populus buds. Detection of several inhibitors of acetyl- and butyrylcholinesterase and rabbit liver esterase in all samples. Bioactive compounds were assigned by HPTLC-MS, e.g. chrysin as selective cholinesterase inhibitor, and caffeic acid and galangin as antimicrobials in (1) and (2). The method is suitable to determine the botanical origin and quality of Populus bud extracts and propolis samples.

      Classification: 13b, 32e
      121 022
      Unprecedented sensitivity of the planar yeast estrogen screen by using a spray-on technology
      A. SCHOENBORN*, P. SCHMID, S. BRÄM, G. REIFFERSCHEID, M. OHLIG, S. BUCHINGER (ZHAW Life Sciences und Facility Management, Grüental, 8820 Wädenswil, Switzerland)

      J. Chromatogr. A 1530, 185-191 (2017). Development of a new spray-on method for applying yeast cells to HPTLC plates, leading to a much higher sensitivity of the planar yeast estrogen screen (p-YES), which can serve as a highly valuable and sensitive screening tool for the detection of estrogenic compounds in various sample matrices such as water and wastewater, personal care products and foodstuff. HPTLC of sample constituents and direct detection of estrogenic compounds by spraying with yeast cells. This resulted in much sharper signals compared to those in previous publications. Satisfying results were achieved using cultures with cell densities of 1000 FAU with reduced signal broadening, thus lower LOQ for estrogenic compounds, e.g. estrone 2 pg/zone, 17β-estradiol 0.5 pg/zone, 17α-ethinylestradiol 0.5 pg/zone and estriol 20 pg/zone. Demonstration of the possibility of the method to characterize profiles of estrogenic activity of wastewater samples with high quality and reproducibility by using native samples from wastewater or even surface water directly applied on HPTLC plates without the need for prior sample treatment.

      Classification: 4e, 13b, 37c
      121 054
      Effect-based and chemical analytical methods to monitor estrogens under the European Water Framework Directive
      Sara KONEMANN*, R. KASE, E. SIMON, K. SWART, S. BUCHINGER, M. SCHLÜSENER, H. HOLLER, B. ESCHER, I. WERNER, S. AÏSSA, E. VERMEIRSSEN, V. DULIO, S. VALSECCHI, S. POLESELLO, P. BEHNISCH, B. JAVURKOVA, O. PERCEVAL, C. DI PAOLO, D. OLBRICH, E. SYCHROVA, R. SCHLICHTING, L. LEBORGNE, M. CLARA, C. SCHEFFKNECHT, Y. MARNEFFE, C. CHALON (*Institute for Environmental Research, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany, sarah.koenemann@rwth-aachen.de)

      Trends Anal. Chem. 102, 225-235 (2018). The study described reliable effect-based methods for screening of endocrine disrupting compounds to fulfill the requirements of the European Decision EU 2015/495 regarding steroidal estrogens of the Water Framework Directive. The study identified TLC-based methods such as the planar Yeast Estrogen Screen (pYES) for the quantification of the overall estrogenic activity present in the sample by means of 17ß-estradiol-equivalence concentrations.

      Classification: 13b
      102 037
      Evaluation of visualizing reagents for estradiol on thin layer by densitometric method
      Alina PYKA*, W. KLIMCZOK, D. GURAK (*Department of Analytical Chemistry, Faculty of Pharmacy, Medical University of Silesia, 4 Jagiellonska Street, 41-200, Sosnowiec, Poland; apyka@slam.katowice.pl)

      J. Liq. Chromatogr. Relat. Technol. 31, 555-566 (2008). Five new derivatization reagents (gentian violet, methylene violet, methylene blue, malachite green, and Janus blue) were used to detect estradiol on aluminium oxide. Barton’s reagent, rhodamine B, and sulfuric acid were used as the comparative derivatization reagents. Limit of detection, detection index, modified broadening index, modified contrast index, and linearity range were determined for estradiol after derivatization with these reagents. Quantitative determination by absorbance measurement between 200 and 600 nm.

      Classification: 13b