Br. by High-Performance Thin-Layer Chromatography. J. Planar Chromatogr. 20, 117-120 (2007). HPTLC of ursolic acid (3beta-hydroxyuro-12-enoic acid) on silica gel prewashed with methanol in a twin-trough chamber with toluene - ethyl acetate - triethylamine - methanol 7:2:1:1. After derivatization with Liebermann-Burchard reagent the chromatograms were evaluated densitometrically at 366 nm in the fluorescence mode.

J. Planar Chromatogr. 22, 183-186 (2009). HPTLC of ethyl 8-oxo-5,6,7,8-tetrahydrothiazolo[3,2-a][1,3]diazepin-3-carboxylate (HIE-124) in plasma (with diazepam as internal standard) on silica gel with chloroform - ethyl acetate 4:1 with chamber saturation for 30 min. Quantitative determination by absorbance measurement at 265 nm. The limit of detection and quantification was 20 µg/L (40 ng/band) and 40 µg/L (80 ng/band), respectively.

J. Planar Chromatogr. 24, 435-440 (2011). TLC of nine N-cyclohexyl-N-substituted-2-phenylacetamides on RP-18 with different aqueous eluents; water - acetone, water - acetonitrile, and water - dioxane. The volume fraction of the organic modifier in the aqueous mobile phase comprised 60-80 % (dioxane, acetone) and 70-90 % (acetonitrile) and varied in steps of 5 %. Detection under UV light at 254 nm. The effects on retention behavior of all investigated modifiers were similar. The linear relationship between retention parameters and organic modifier content allowed the extrapolation procedure. The result of the investigation showed that reversed-phase RM0 proved to express the lipophilic nature of an investigated compound as well as its biological activity.

J. Planar Chromatogr. 27, 107-112 (2014). HPTLC of disulfiram on RP-18 with methanol - water 9:1. Detection by spraying with copper(II) sulfate (0.5 mol/L), followed by heating at 50 ºC for 2 min. Quantitative evaluation with an office scanner at 300 dpi followed by image processing with software. The hRf value for disulfiram was 67. Linearity was in the range of 1-10 μg/zone. The intermediate/interday/intra-day precisions were below 1 % (n=6). The LOD and LOQ were 260 and 860 ng/zone, respectively. Recovery was in the range of 92.4-102.0 %.

J. Chromatogr. A 1530, 192-196 (2017). HPTLC to measure the antioxidant and hypoglycemic effects in different extracts from Myrmecodia platytyrea, which can be a traditional medicine as alternative treatment for diabetes, because the substances produced by ants can reduce blood sugar levels. Measurement of the antioxidant activity in methanol, ethanol, dichloromethane (DCM) and ethyl acetate (EA) extracts with the HPTLC-2,2-diphenyl-1-picrylhydrazyl free radical (DPPH*) assay, and of the hypoglycemic effects with a newly developed α-amylase inhibitory activity assay. Detection of stigmasterol after derivatization with anisaldehyde reagent as purple colored zone under white light at hRF 66. The results showed the highest antioxidant activity in the ethanol extract rich in polyphenols and flavonoids. No antioxidant activity but significant α-amylase inhibitory activity was observed in the DCM extract. The highest α-amylase inhibitory activity was found in the EA and DCM extracts and was related to their stigmasterol content.

Lipids 18, 87-89 (1983). The chromatographic mobilities of 17 sterols and squalene on reversed-phase TLC plates with 4 nonaqueous solvent systems is described. Solvent Systems: hexane - ethyl acetate 9:1 and other systems were used. Visualization: 10 % phosphomolybdic acid in 95 % ethanol, followed by heating at 100 °C; iodine vapor; 10 % sulfuric acid in 50 % methanol, followed by heating at 100 °C.

J. Chromatogr. 362, 303-307 (1986). TLC of 18 sulfonamides and a related drug (trimethoprim) on silica with ethyl acetate - methanol 9:1. Detection with 0.5 % 2,5-dichloro-p-benzoquinone in DMSO. This spray reagent produces very rapidly sharp colors which are highly stable. See also: S. P. Agarwal and J. Nwaiwu, J. Chromatogr. 351, 383-387 (1986).

Anal. Biochem. 156, 447-453 (1986). Two-dimensional TLC of 4 phosphoinositide cycle phospholipids and commonly encountered major and minor phospholipids on silica with chloroform - methanol - acetic acid 55:25:5. The method was applied successfully to human erythrocytes, human platelets and BL/VL3 murine lymphoma cells.