Biomed. Chromatogr. 10, 225-227 (1996). TLC on silica with chloroform - acetone - ethyl acetate - methanol 136:64:4:1. Detection by spraying with ethylenediamine - methanol - potassium ferricyanide (1.5%) 1:3:1, incubating for 5 min at 60°C and in the dark at room temperature for 15 min. Quantification by fluoro densitometry at 415/>485 nm.

J. Planar Chromatogr. 17, 255-260 (2004). TLC of fourteen 1-arylpiperazine derivatives on silica gel with monocomponent and binary non-polar mobile phases and on RP-18 with binary mobile phases comprising mixtures of methanol, acetone, or dioxane (as organic modifiers) and water in a horizontal chamber after equilibration for 15 min. Detection under UV light at 254 nm.

J. Planar Chromatogr. 20, 203-207 (2007).. HPTLC of epalrestat (5-[(1Z,2E)-2-methyl-3-phenylpropenylidene]-4-oxo-2-thioxo-3-thiazolidineacetic acid) with nitrofurantoin as internal standard on silica gel with ethyl acetate - toluene - acetic acid 30:20:1. Densitometric scanning in absorbance mode at 290 nm.

Anal. Chem. 81, 10275-10284 (2009). Direct quantitative bioanalysis of drugs from dried blood spot samples using a TLC-MS interface with or without HPLC separation. The method gave acceptable sensitivity, linearity, accuracy, and precision data for bioanalytical validations. The direct elution technique was shown to increase assay sensitivity for a range of analytes. Investigations were performed to optimize extraction time, minimize sample-to-sample carry-over, and compare chromatographic performance. On the basis of this preliminary assessment, it has been demonstrated that this TLC-MS interface has the potential to be an effective tool for the direct analysis of drugs in dried blood samples at physiologically relevant concentrations.

J. of Chromatogr. A 1218 (20), 3089-3084 (2011). Comparison of the separation of the structurally related angiotensin-converting enzyme (ACE) inhibitors lisinopril, cilazapril, ramipril and quinapril and their corresponding active diacid forms (prilates) by conventional TLC on silica gel with the separation on monolithic ultra-TLC (UTLC) phase. Technical modifications of the commercially available equipment for sample application, development and detection were necessary for the use with UTLC plates. Development in a modified horizontal developing chamber with ethyl acetate - acetone - acetic acid - water 16:4:1:2. Detection by absorbance measurement at 220 nm and after exposure to iodine vapors under daylight, as well as by image analysis. As a result the monolithic layer was more efficient for the separation of structurally similar polar compounds, such as prilates, than conventional silica layers. Confirmation of the identity of the compounds by ESI-MS after their online extraction from the UTLC and TLC plates.

extract and its traditional formulation from rat plasma; application to pharmacokinetics. J. Planar Chromatogr. 27, 23-28 (2014). HPTLC of marmelosin in plasma on silica gel with toluene - ethyl acetate - glacial acetic acid 70:30:1. Quantitative determination by absorbance measurement at 310 nm. The hRF value for marmelosin was 57. Linearity was between 1 and 150 µg/mL. The intermediate/interday/intra-day precisions were below 6 % (n=6). The LOD and LOQ for marmelosin was 150 ng/mL and 1000 ng/mL, respectively. Recovery was in the range of 90.9-106.1 %.

J. Chromatogr. Sci. 56 (1), 81-91 (2018). HPTLC of mixures of tamsulosin hydrochloride (TAM) with either tolterodine tartrate (TOL) or solifenacin succinate (SOL) in bulk drug and in combined dosage forms on on silica gel with ethyl acetate – methanol – ammonia 120:80:1. Quantification by densitometry at 224 nm. Linearity was between 0.1-0.7, 0.4-4 and 1-6 μg/band for TAM, TOL and SOL, respectively.

Chromatography the state of the Art, Vol.1. Akademiai Kiado, Budapest 1985, 187-194. OPTLC on cation ion exchange layer with 300 mmol/l potassium phosphate buffer pH 7.5 containing 2 mmol/l NaCl. Separation of methylamine, ornithine, arginine, cadaverine, putrescine, spermidine, agmatine, spermine. Videodensitometry after staining with ninhydrin. Limit of determination 2-60 nmol / spot with 3 % error.