Contribution to the investigation of 7,8-dihydropteroate synthase. J. Chromatogr. 659, 185-189 (1994). Evaluation of the 7,8-dihydropteroate synthase reaction via determination of the stable pteroic acid from oxidation of the enzymatic product dihydropteroic acid. TLC on amino-bonded silica with a solution of 52% acetonitrile in 100 mM tris-HCl buffer (pH 8.6). Quantification by densitometry. Detection limit: at levels down to 0.5 ng/µL.

Prostaglandins Leukotr Essent Fatty Acids 52, 223-227 (1995). TLC of hydroxy derivatives of arachidonic acid (12-hydroxyheptadecatrienoic acid and 12-hydroxyeicosatetraenoic acid), thromboxane B2, prostaglandins and phospholipids on silica with the upper organic phase of ethyl acetate - isooctane - acetic acid - water 11:5:2:10 or hexane - ether - acetic acid 80:30:1. Detection by exposure to iodine vapor. After marking the spots were scraped off and counted for radioactivity in a liquid scintillation counter.

Chromatogr. (Sepu) 14, 259-263 (1996). Two dimensional TLC of 30 amino acids, 40 organic acids and 20 nucleosides and related compounds on cellulose - silica with 1) isopropanol - ethyl acetate - acetone - methanol - para-amyl alcohol - NH3 - water 9:3:3:1:1:3:3, butanol - isopropanol - acetone - formic acid - water 18:8:8:3:6, 2) tert-butanol - ethanol - NH3 - water 5:6:1:3, isopentanol - petrol ether - ethanol - formic acid - propionic acid - water 45:100:25:20:1:1, 3) isopropanol - ethyl acetate - methanol - NH3 - water 5:3:1:3:1, isopropanol - saturated (NH4)2SO4 - water 4:77:19, for both direction, respectively. Detection under UV 254 nm, by spraying with 0.1 % acridine in ethanol, by spraying with ninhydrin-cadmium acetate reagent. Detection limit 10-10 - 10-11 mol, 2x10-8 - 5x10-8 mol, and 1x10-8 - 3x10-8 mol, respectively.

Acta Chromatographica 14, 92-101 (2004). Study of the chromatographic behavior of some cephalosporins on synthetic inorganic ion-exchange (stannic oxide) layers with citrate and borate buffers as mobile phases. Separation of cephalosporins in synthetic mixtures and quantification by spectrophotometry. Quantitative determination of cephalosporins in blood serum samples from patients. Statistical discussion of recovery, accuracy, and reproducibility of the method.

J. Liq. Chromatogr. & Relat. Technol. 29, 2753-2765 (2006). TLC of tadalafil [6R,12aR)-2,3,6,7,12a-hexahydro-2-methyl-6-[3,4-(methylenedioxy)phenyl]pyrazino-[1’,2’:1,6]pyrido[3,4-b]indole-1,4-dione] on silica gel with n-hexane - ethyl acetate - methanol 4:3:1. Quantitative determination by absorbance measurement at 285 nm.

J. Planar Chromatogr. 20, 39-42 (2007). Displacement TLC of quaternary pyridinium aldoximes (e. g. pralidoxime and obidoxime) on silica gel impregnated with paraffin oil by continous development with 10 % paraffin oil in n-hexane for 18 h with water - acetone - hydrochloric acid 8:1:1. Detection under UV light at 254 nm.

Anal. Chem. 79, 2101-2108 (2007). TLC of benzodiazepines (midazolam, diazepam, lorazepam, oxazepam, N-desalkyl-flurazepam, triazolam, nitrazepam) on monolithic UTLC phase (prewashed with methanol) with dichloromethane - acetone 93:7 for one-dimensional and toluene - acetone - ethanol - 25 % ammonia 70:20:3:1 for two-dimensional separation in a saturated chamber. Developing distance 2 cm; separation time for 1-D chromatography 2-6 min, for 2-D 4-12 min. Quantitative determination by absorbance measurement at 254 nm; for visual detection the analytes were derivatized by spraying with Dragendorff reagent. Identification of the separated compounds by AP-MALDI-MS. The limits of detection were in the picomole range and thus low enough for bioanalysis.

Pharmacie Globale (IJCP) 4(05), 1-4 (2011). TLC of cefixime and ofloxacin in combined tablet dosage formulation on silica gel with n-butanol - 25% ammonia - water - DMSO 8:3:1:2. The hRf value of cefixime was 55 and of ofloxacin 65. Quantitative evaluation by absorbance measurement at 297 nm. The method was linear in the range of 30-180 ng/band for both drugs.