Abstract G-33, IPC (2005). HPTLC of epalrestat in plasma on silica gel with ethyl acetate - toluene - acetic acid 30:20:1. Nitrofuranloin was used as internal standard. Quantitative determination by absorbance measurement at 390 nm. Linearity was obtained the range of 0.01-0.20 µg/mL with recovery of 99-107 %. The method was validated as per ICH guidelines.

J. AOAC Int. 91, 1218-1226 (2008). HPTLC of glycosphingolipids on silica gel with chloroform - methanol - water 60:35:8 or 65:25:4. Detection by spraying with orcinol reagent followed by heating at 110 °C. Detection with HPTLC/MS by direct coupling of HPTLC to matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry showed to be a reliable and reproducible method to obtain structural information and fundamental properties of glycosphingolipids.

IPA Convention, 2010, RA-PO 03. HPTLC of edaravone in samples of human plasma (purified by liquid-liquid extraction) on RP-18 with n-butanol - methanol - diethyl ether 1:8:1. The compound was well resolved with an hRf value of 81. Densitometric evaluation at 240 nm. The limit of detection and quantification was 25 ng and 150 ng respectively. The linearity was 600-2400 ng/band. Recovery (%) based on analysis of spiked sample was more than 65 %.

J. Ethnopharmacol. 137, 99-104 (2011). HPTLC of karanjin in rabbit plasma on silica gel with dichloromethane - toluene - methanol 35:15:3. Qualitative evaluation under UV 366 nm and quantitative determination by densitometry at 366 nm. Linearity was between 1.0 and 15.0 µg/mL. LOD and LOQ were 0.5 and 1.0 µg/mL, respectively. The inter-day and intra-day accuracies were 97.1 % and 92.2 %, respectively. Recovery (by standard addition) was between 98.2 % and 99.9 %.

J. Planar Chromatogr. 27, 416-419 (2014). HPTLC of (1) cotinine and (2) trans-3'-hydroxycotinine in urine on RP-18 with acetonitrile - water 4:1. Quantitative determination by absorbance measurement at 260 nm. Linearity was in the range of 65-325 ng/zone (at a low calibration range) and 325-1300 ng/zone (at a high calibration range) for (1) and 62.5-312.5 and 312.5-1250 ng/zone for (2). The intermediate precisions were below 9 % (n=3). The LOD and LOQ were 13 and 38 ng/zone for (1) and 17 and 50 ng/zone for (2), respectively.

J. Planar Chromatogr. 31, 265-271 (2018). HPTLC of aspirin (1), paracetamol (2), and caffeine (3) in saliva, after ultrasound-assisted emulsification microextraction, with chloroform on silica gel with ethyl acetate - acetic acid 19:1. Detection under UV light at 254 nm. Quantitative determination using ImageJ software. The hRF values for (1) to (3) were 90, 80 and 50, respectively. Linearity ranged between 4 and 200 μg/zone for (1) to (3). LOD and LOQ were 12 and 38 μg/zone for (1), 8 and 26 μg/zone for (2), and 7 and 23 μg/zone for (3), respectively. The intermediate precision was <10 % (n=3). Recovery was in the range of 89-94 % in saliva for (1) to (3).

V. Danube Symposium on Chromatography Yalta, November 11 -16, 1985. Samples of 100 ml urine acidified with acetic acid, urineporphyrins adsorbed on talcum. TLC of methylesters on silica. Densitometry by fluorescence.

J. Biochem. (Tokyo) 99, 771-775 (1986). Reversed-phase TLC of cholestanol after conversion to alpha and beta -epoxides with m-chloroperbenzoic acid. Detection by spraying with phosphomolybdic acid. Quantification by densitometry. The calibration curves were linear 100-1000 ng. Correlation among TLC, GC/MS, and GC methods was 1:1:1. TLC method is useful for primary diagnostic screening of CTX.