) Roxb by HPTLC method. 59th Indian Pharmaceutical Congress C-100, 248, (2007). An HPTLC method has been developed for the estimation of flavonoids tannin and saponin in Caesalpinia bonduc. The dried powdered leaves of the plant were extracted with methanol and used for evaluation. HPTLC on silica gel with ethyl acetate - formic acid - glacial acetic acid - water 20225 for flavonoids, chloroform - glacial acetic acid - methanol - water 16832 for saponin, and ethyl acetate - toluene - formic acid 661 for tannin. Detection by spraying with 5 % methanolic sulphuric acid for saponin, 5 % alcoholic aluminium chloride solution for flavonoids, and 5 % ferric chloride solution for tannin. Densitometric evaluation at 254 nm and 366 nm.

Whole Plant Powder by HPTLC. Chromatographia 65 (11-12), 767-769 (2007). HPTLC of rutin in the whole plant powder of Amaranthus spinosus Linn. on silica gel with ethyl acetate - formic acid - methanol - distilled water 10091117. Quantification by densitometry at 363 nm. Linearity was between 10 and 60 µg/mL for rutin. The concentration of rutin in the whole plant powder was found to be 0.15 %.

Acta Chromatographica 21 (1), 83-93 (2009). HPTLC of quetiapine fumarate on silica gel with toluene - methanol 41. The hRf value of quetiapine fumarate was 37. Quantitative determination by absorbance measurement at 254 nm. There was no chromatographic interference from tablet excipients. The drug is susceptible to treatment with acid and alkaline hydrolysis, oxidation, and photodegradation. The method was able to separate the degradation products from the pure drug, it can be used for stability tests.

Chinese J. Pharm. Anal. 28 (1), 20-23 (2008). TLC of TCM drug extracts on silica gel with toluene – ethyl acetate – methanol – isopropanol – ammonia 126331. Detection under UV light. The method is suitable for quality control of Baozhi pills.

Journal of Pharmaceutical Research 9(2), 60-62 (2010). TLC on silica gel with chloroform - ethyl acetate - methanol - ammonia 2020101 with chamber saturation for 30 min. Densitometric evaluation at 232 nm. The method was linear in the range of 100-800 ng/band for amlodipine, 400-2400 ng/band for hydrochlorothiazide and 1000-8000 ng/band for amlodipine. The average recovery was 99.1-100.4 % for all three compounds. The method was suitable for stability studies (stress conditions acid, base, thermal, UV).

Acta Chromatographica 22 (4), 549-567 (2010). HPTLC of risperidone on silica gel with methanol - ethyl acetate 41. The hRf value of risperidone was 34. Quantitative evaluation by absorbance measurement at 285 nm. The linearity was in the range of 100-600 ng/band (r=0.9996), the LOD was 22 ng/band and the LOQ was 68 ng/band. The method was suitable for selective analysis of risperidone and was successfully used for estimation of the equilibrium solubility of risperidone, and for quantification of risperidone as the bulk drug in a commercially available preparation, in in-house developed mucoadhesive microemulsion formulations, and in solution.

J. Chromatogr. Sci. 54 (1), 36-42 (2016). Description of a method for simultaneous determination of cinnarizine (CIN) and dimenhydrinate (DIM) by TLC on silica gel with ethyl acetate – methylene chloride 41, detection and quantification by densitometry at 254 nm. Linearity was in the range of 0.5-6 µg/band for CIN and 1-6 µg/band for DIM. The mean recovery was 100 % for both substances. The methods were applied to the simultaneous determination of CIN and DIM in bulk powder, laboratory-prepared mixtures and pharmaceutical dosage forms.

J. Chromatogr. Sci. 55, 961-968 (2017). Presentation of two accurate, precise and highly selective stability-indicating methods for simultaneous determination of benztropine mesylate (BNZ) in presence of its hepatotoxic and carcinogenic degradation product, benzophenone (BPH) either in pure form or in the pharmaceutical formulation without any preliminary separation steps. TLC of BNZ and its degradation product on silica gel with hexane – methylene chloride – triethylamine 25253. Quantitative determination by densitometry at 235 nm. The linearity was between 1.5-10 ?g/band and 1-10 ?g/band for BNZ and BPH, respectively. UPLC of the mixture on a RP C8 analytical column, quantification using a diode array detector at 210 nm. The linearity with UPLC was 20-200 ?g/mL and 5-50 ?g/mL for BNZ and BPH, respectively. Comparison of the results showed no significant differences between the TLC and UPLC method regarding both accuracy and precision.