Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      130 004
      Identification of acetylcholinesterase inhibitors in water by combining two-dimensional thin-layer chromatography and high-resolution mass spectrometry
      Lena STÜTZ*, W. SCHULZ, R. WINZENBACHER (*Laboratory for Operation Control and Research, Zweckverband Landeswasserversorgung, Langenau, Germany;

      J Chromatogr A, 1624, 461239 (2020). Samples were chemical standards of acetylcholinesterase (AChE) inhibitors (azamethiphos, caffeine, donepezil, galanthamine, methiocarb-sulfoxide, paraoxon-ethyl) and of neurotoxic compounds, as well as drinking or contaminated water samples enriched through solid phase extraction. HPTLC on spherical silica gel (pre-washed twice by 20 min immersion in isopropanol, heated 20 min at 120 °C before and after pre-washing with acetonitrile). First separation (preparative TLC) with automated multiple development (16 steps). Effect-directed analysis for AChE inhibitors by immersion (speed 5 cm/s, time 1 s) into enzyme solution, incubation 5 min at 37 °C and immersion into substrate solution (indoxyl acetate 2 % in methanol); visualization under UV 366 nm. Active zones from untreated layers were eluted through the oval head of a TLC-MS interface to a second plate for a second separation with a panel of other mobile phases. Bands of interest were eluted from the second layer with water through the oval elution head of the TLC-MS interface pump, into a RP18 liquid chromatography guard column, followed by a quadrupole time-of-flight mass spectrometer. Full scan mass spectra (m/z 100–1200) were recorded in negative and positive modes using electrospray ionization (and collision-induced dissociation for MS2). Among the water contaminants, lumichrome (riboflavin photolysis product), paraxanthine and linear alkylbenzene sulfonates were identified as AChE inhibitors.

      Classification: 3d, 4d, 4e, 22, 29b, 35d, 37c
      130 005
      Multiobjective optimization of microemulsion – thin layer chromatography with image processing as analytical platform for determination of drugs in plasma using desirability functions
      Noura H. ABOU-TALEB*, D. T. EL-SHERBINY, N. M. EL-ENANY, H. I. EL-SUBBAGH (*Medicinal Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt;

      J Chromatogr A, 1619, 460945 (2020). Samples were lamotrigin as standard, or extracted with an oil-in-water microemulsion (10 µL butyl acetate, 4 mL n-butanol, 925 mg sodium dodecyl sulphate, 8.6 mL water) either from patients’ raw plasma (for separation from blood proteins) after spiking, or from commercial tablets dissolved in methanol. TLC on silica gel with a water-in-oil microemulsion of 9 mL butyl acetate, 1 mL n-butanol, 250 mg sodium dodecyl sulphate, 250 µL water. Both optimal microemulsions were predicted using Taguchi orthogonal array and Plackett-Burman design. Evaluation in UV 254 nm, quantification from the digital picture using four image processing software programs. For lamotrigin (hRF 24), limits of quantification were 170 ng for pure drug and 10 ng for spiked plasma. Linearity (in range 20–200 ng/spot) was directly obtained for the calibration curve in spiked plasma; however, for pure drug, linearity was obtained only when using log values of the calculated densities (300–3000 ng/spot).

      Classification: 3a, 3d, 5c, 23e, 32c
      130 136
      Complementary developing solvents for simpler and more powerful routine analysis by high‑performance thin‑layer chromatography
      Tien DO*, M. SCHMID, I. TRETTIN, M. HäNNI, E. REICH (*CAMAG, Sonnenmattstrasse 11, 4132 Muttenz, Switzerland,

      J. Planar Chromatogr. 35, 299-311 (2022).  HPTLC of powdered herbal drugs and finished products (leaves of Mentha piperita, Olea oleuropea, Ginkgo biloba and Camellia sinensis, fruits of Styphnolobium japonicum and Piper nigrum, roots of Angelica species (A. gigas, A. sinensis, A. dahurica, A. acutiloba, and A. pubescens, Curcuma longa and poly-herbal products containing powdered extracts of Curcuma longa root and Piper nigrum fruits) on silica gel with three complementary developing solvents (CDS): low polar developing solvent (toluene - ethyl acetate 9:1); medium polar developing solvent (cyclopentyl methyl ether - tetrahydrofuran - water - formic acid 40:24:1:1); and high polar developing solvent (ethanol - dichloromethane - water - tetrahydrofuran 16:16:4:1). Detection by heating at 100 °C for 3 min, followed by spraying with NP reagent (1.0 g of 2-aminoethyl diphenylborinate in 100 mL of methanol). For Olea oleuropea and Ginkgo biloba, the derivatization with NP was followed by spraying with anisaldehyde sulfuric acid reagent and heating at 100 °C for 3 min. Analysis was performed under UV light at 254 and 366 nm. Performance of the Universal HPTLC mix (UHM) was assessed in terms of precision. The hRF values for all substances were between 20 and 80. 

      Classification: 3d
      130 137
      Screening of orange peel waste on valuable compounds by gradient multiple development diode‑array high‑performance thin‑layer chromatography
      B. SPANGENBERG*, A. SEIGEL, R. BRAMER (*Institute of Process Engineering, Offenburg University of Applied Sciences: Hochschule Offenburg, Badstrasse 24, 77652 Offenburg, Germany,

      J. Planar Chromatogr. 35, 313-330 (2022).  HPTLC of orange peel extract on silica gel with gradient multiple development using seven different polarity ranges: cyclohexane, cyclohexane - n-heptane 3:7, cyclohexane - methyl tert-butyl ether 43:7, cyclohexane - methyl tert-butyl ether 7:3, cyclohexane - methyl tert-butyl ether 3:7, methyl tert-butyl ether, methyl acetate - ethanol 9:1, ethyl acetate - ethanol - formic acid 44:5:1. Detection by spraying with vanillin reagent (100 mg vanillin dissolved in 9.8 mL ethanol and 0.2 mL sulfuric acid), followed by heating at 100 °C for 2 min. DPPH staining was performed with 2 mL of a DPPH solution (15 mg dissolved in 10 mL of methanol). Bioautography was performed by dipping into Aliivibrio fischeri bacteria suspension for 6 s, followed by measurement of bioluminescence within 15 min. In this sample, more than 50 compounds could be separated.

      Classification: 3d, 32e
      130 138
      Influence of starting zone on the efficiency of micro‑thin‑layer chromatography with controlled mobile phase flow rate
      Aneta GRYSINSKA*, A. KARDASZ, T. DZIDO (*Department of Physical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland,

      J. Planar Chromatogr. 35, 243-250 (2022). Micro TLC of three dyes (1-aminoanthraquinone, fat green and 2-nitroaniline) on silica gel with toluene at distances 1, 1.5, 2, 2.5, or 3 cm. Experiments were performed using a prototype device operated at a controlled velocity of the mobile phase, where the chromatographic plate was placed in the chamber with the adsorbent layer face-down and the mobile phase was delivered onto the adsorbent layer of the chromatographic plate by the pipette, which was driven into movement by a 3D machine controlled by a computer. Different solvents (acetone, methanol, toluene, or heptane) were used to wet and to narrow the starting zones. Detection under UV light at 286 nm. To take full advantage of the benefits of micro-planar chromatography, the size of the starting zone should be reduced as well as the processes
      related to the dissolution kinetics of the starting zones of substances in the mobile phase should be optimized.

      Classification: 3d
      130 017
      Thin-layer chromatography gradient optimization strategy for wet load adsorption flash chromatography
      P. KRECISZ, K. CZARNECKA, P. SZYMANSKI* (*Dep. of Pharm. Chem., Drug Anal. & Radiopharmacy, Med. Univ. of Lodz, ul. Muszynskiego 1, 90-151 Lodz, Poland, or Dep. of Radiobiol. & Rad. Prot., Milit. Inst. of Hygi. & Epidemiol., 4 Kozielska St., 01-163 Warsaw, Poland,

      J Chromatogr Sci, 60(5), 472-477 (2022). Discussion of the extensive use of reversed phase chromatography in analytical and preparative applications in modern pharmaceutical industry and science, stressing a special place of the normal phase adsorption chromatography in purifying post-reaction mixtures or the separation of natural extracts, especially in wet load mode due to its simplicity and high velocity of preparation. The presented TLC gradient optimization strategy for wet load separations yields repeatable results of separations for different compounds without worrying about negative impact of wet loading on separation quality.The strategy uses an elution model of the desired compound, which is used to develop the gradient method. I also allows to standardize the separation time, because gradient methods performed by the TLC gradient optimization strategy have a similar duration time in column volumes. Therefore the method can simply be scaled using the column volume as a base unit in calculations.

      Classification: 3d
      129 071
      Comparison of high-performance thin-layer with overpressured layer chromatography combined with direct analysis in real time mass spectrometry and direct bioautography for tansy root
      Ágnes M. MÓRICZ*, T.T. HÄBE, P.G. OTT, G.E. MORLOCK
      (*Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, 1022 Budapest, Hungary;

      J Chromatogr A, 1603, 355–360 (2019). Samples were ethyl acetate root macerates of fully flowered Tanacetum vulgare (Asteraceae). HPTLC on silica gel (classical irregular particles vs. Lichrosphere with spherical particles) previously washed with methanol, dried for 5 min at room temperature, perimeter-sealed with a polymer coat, and heated for 30 min at 100 °C. Separation with toluene or with toluene – n-hexane 7:3, in classical capillary flow or in OPLC (overpressured layer chromatography). For OPLC, off-line infusion was used (closed mobile phase (MP) outlet, automatically stopping development); external pressure 50 bar, rapid MP flush 175 and 350 µL, MP flow rate 250 and 500 µL/min, 1830 and 3475 µL MP, development time 446 s and 424 s. Derivatization by immersion into vanillin – sulfuric acid reagent, followed by 5 min heating at 110 °C; or into PABA reagent (500 mg p-aminobenzoic acid, 18 mL glacial acetic acid diluted, 20 mL water, 1 mL o-phosphoric acid, 60 mL acetone), followed by 5 min heating at 140 °C. Effect-directed analysis using automated immersion: A) for free radical (DPPH•) scavengers; B) for activity against Gram-negative bacteria using Aliivibrio fischeri bioluminescence assay; C) for activity against Gram-positive bacteria with Bacillus subtilis bioassay. Four active polyynes were identified as hexadiynylidene-epoxy-dioxaspiro-decane (1), pontica epoxyde (nonene-triynyl-vinyl-oxirane) (2), tetradeca-triine-en-one (3) and trans-(hexadiynylidene)-dioxaspiro-decene (4), by hyphenating OPLC to quadrupole-orbitrap HRMS without eluent, using a DART interface (Direct Analysis in Real-Time, needle voltage 4kV, grid voltage 50 V, helium as gas, temperature 500 °C, full scan in positive ionization mode in m/z range 100-750). Polyynes (3) and (4) were coeluting in HPTLC but not in OPLC, demonstrating that (4) is not produced by oxidation during the DART-MS procedure. Separation with OPLC compared to HPTLC was performed in a shorter time and with better resolution at the same time. Layers with spherical particles gave higher resolution; zone distortions occurring in OPLC due to dissolved air in MP were prevented by previous MP sonication.

      Classification: 3b, 3d, 4e, 5a, 8b, 9, 32e
      125 039
      Preliminary results of layer chromatography combined with bilateral band compression to search active minor components
      Agnes MORICZ*, E. MINCSOVICS, P. OTT (*Plant Protection Institute, Centre for Agricultural Research, Budapest 1022, Hungary,

      J. Liq. Chromatogr. Relat. Technol. 43, 300-304 (2020). HPTLC of thymol, carvacrol and linalool in Solidago canadensis on silica gel with n-hexane - acetone 4:1. The method was compared with bilateral band compression (BBC) of the 10 mm wide lanes of HPTLC separation, resulting in more than 6 times increase in peak height and peak area. In BBC a solvent flow perpendicular to the direction of chromatogram development squeezes the chromatographic bands into a smaller area The method improved detection sensitivity of sample components with low abundance.

      Classification: 3d