Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      130 004
      Identification of acetylcholinesterase inhibitors in water by combining two-dimensional thin-layer chromatography and high-resolution mass spectrometry
      Lena STÜTZ*, W. SCHULZ, R. WINZENBACHER (*Laboratory for Operation Control and Research, Zweckverband Landeswasserversorgung, Langenau, Germany; stuetz.l@lw-online.de)

      J Chromatogr A, 1624, 461239 (2020). Samples were chemical standards of acetylcholinesterase (AChE) inhibitors (azamethiphos, caffeine, donepezil, galanthamine, methiocarb-sulfoxide, paraoxon-ethyl) and of neurotoxic compounds, as well as drinking or contaminated water samples enriched through solid phase extraction. HPTLC on spherical silica gel (pre-washed twice by 20 min immersion in isopropanol, heated 20 min at 120 °C before and after pre-washing with acetonitrile). First separation (preparative TLC) with automated multiple development (16 steps). Effect-directed analysis for AChE inhibitors by immersion (speed 5 cm/s, time 1 s) into enzyme solution, incubation 5 min at 37 °C and immersion into substrate solution (indoxyl acetate 2 % in methanol); visualization under UV 366 nm. Active zones from untreated layers were eluted through the oval head of a TLC-MS interface to a second plate for a second separation with a panel of other mobile phases. Bands of interest were eluted from the second layer with water through the oval elution head of the TLC-MS interface pump, into a RP18 liquid chromatography guard column, followed by a quadrupole time-of-flight mass spectrometer. Full scan mass spectra (m/z 100–1200) were recorded in negative and positive modes using electrospray ionization (and collision-induced dissociation for MS2). Among the water contaminants, lumichrome (riboflavin photolysis product), paraxanthine and linear alkylbenzene sulfonates were identified as AChE inhibitors.

      Classification: 3d, 4d, 4e, 22, 29b, 35d, 37c
      130 005
      Multiobjective optimization of microemulsion – thin layer chromatography with image processing as analytical platform for determination of drugs in plasma using desirability functions
      Noura H. ABOU-TALEB*, D. T. EL-SHERBINY, N. M. EL-ENANY, H. I. EL-SUBBAGH (*Medicinal Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt; nourahemdan@yahoo.com)

      J Chromatogr A, 1619, 460945 (2020). Samples were lamotrigin as standard, or extracted with an oil-in-water microemulsion (10 µL butyl acetate, 4 mL n-butanol, 925 mg sodium dodecyl sulphate, 8.6 mL water) either from patients’ raw plasma (for separation from blood proteins) after spiking, or from commercial tablets dissolved in methanol. TLC on silica gel with a water-in-oil microemulsion of 9 mL butyl acetate, 1 mL n-butanol, 250 mg sodium dodecyl sulphate, 250 µL water. Both optimal microemulsions were predicted using Taguchi orthogonal array and Plackett-Burman design. Evaluation in UV 254 nm, quantification from the digital picture using four image processing software programs. For lamotrigin (hRF 24), limits of quantification were 170 ng for pure drug and 10 ng for spiked plasma. Linearity (in range 20–200 ng/spot) was directly obtained for the calibration curve in spiked plasma; however, for pure drug, linearity was obtained only when using log values of the calculated densities (300–3000 ng/spot).

      Classification: 3a, 3d, 5c, 23e, 32c
      129 071
      Comparison of high-performance thin-layer with overpressured layer chromatography combined with direct analysis in real time mass spectrometry and direct bioautography for tansy root
      Ágnes M. MÓRICZ*, T.T. HÄBE, P.G. OTT, G.E. MORLOCK
      (*Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, 1022 Budapest, Hungary; moricz.agnes@atk.hu)

      J Chromatogr A, 1603, 355–360 (2019). Samples were ethyl acetate root macerates of fully flowered Tanacetum vulgare (Asteraceae). HPTLC on silica gel (classical irregular particles vs. Lichrosphere with spherical particles) previously washed with methanol, dried for 5 min at room temperature, perimeter-sealed with a polymer coat, and heated for 30 min at 100 °C. Separation with toluene or with toluene – n-hexane 7:3, in classical capillary flow or in OPLC (overpressured layer chromatography). For OPLC, off-line infusion was used (closed mobile phase (MP) outlet, automatically stopping development); external pressure 50 bar, rapid MP flush 175 and 350 µL, MP flow rate 250 and 500 µL/min, 1830 and 3475 µL MP, development time 446 s and 424 s. Derivatization by immersion into vanillin – sulfuric acid reagent, followed by 5 min heating at 110 °C; or into PABA reagent (500 mg p-aminobenzoic acid, 18 mL glacial acetic acid diluted, 20 mL water, 1 mL o-phosphoric acid, 60 mL acetone), followed by 5 min heating at 140 °C. Effect-directed analysis using automated immersion: A) for free radical (DPPH•) scavengers; B) for activity against Gram-negative bacteria using Aliivibrio fischeri bioluminescence assay; C) for activity against Gram-positive bacteria with Bacillus subtilis bioassay. Four active polyynes were identified as hexadiynylidene-epoxy-dioxaspiro-decane (1), pontica epoxyde (nonene-triynyl-vinyl-oxirane) (2), tetradeca-triine-en-one (3) and trans-(hexadiynylidene)-dioxaspiro-decene (4), by hyphenating OPLC to quadrupole-orbitrap HRMS without eluent, using a DART interface (Direct Analysis in Real-Time, needle voltage 4kV, grid voltage 50 V, helium as gas, temperature 500 °C, full scan in positive ionization mode in m/z range 100-750). Polyynes (3) and (4) were coeluting in HPTLC but not in OPLC, demonstrating that (4) is not produced by oxidation during the DART-MS procedure. Separation with OPLC compared to HPTLC was performed in a shorter time and with better resolution at the same time. Layers with spherical particles gave higher resolution; zone distortions occurring in OPLC due to dissolved air in MP were prevented by previous MP sonication.

      Classification: 3b, 3d, 4e, 5a, 8b, 9, 32e
      125 039
      Preliminary results of layer chromatography combined with bilateral band compression to search active minor components
      Agnes MORICZ*, E. MINCSOVICS, P. OTT (*Plant Protection Institute, Centre for Agricultural Research, Budapest 1022, Hungary, moricz.agnes@agrar.mta.hu)

      J. Liq. Chromatogr. Relat. Technol. 43, 300-304 (2020). HPTLC of thymol, carvacrol and linalool in Solidago canadensis on silica gel with n-hexane - acetone 4:1. The method was compared with bilateral band compression (BBC) of the 10 mm wide lanes of HPTLC separation, resulting in more than 6 times increase in peak height and peak area. In BBC a solvent flow perpendicular to the direction of chromatogram development squeezes the chromatographic bands into a smaller area The method improved detection sensitivity of sample components with low abundance.

      Classification: 3d
      100 009
      Indirect resolution of enantiomers of penicillamine by TLC and HPLC using Marfey's reagent and its variants
      R. BHUSHAN*, H. BRÜCKNER, V. KUMAR (*Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247 667, India)

      Biomed. Chromatogr. 21 (10), 1064-1068 (2007). Indirect chiral separation of penicillamine (3,3-dimethylcysteine) enantiomers after derivatization with Marfey's reagent (FDNP-Ala-NH2) and two of its structural variants, FDNP-Phe-NH2 and FDNP-Val-NH2, with phenol - water 3:1 and solvent combinations of acetonitrile and triethylamine phosphate buffer in normal and reversed-phase TLC, respectively. Also separation of the diastereomers on a reversed-phase HPLC column with gradient elution of acetonitrile and 0.01 m trifluoroacetic acid. Comparison of the results due to these three reagents. Successful application of the method for checking the enantiomeric impurity of l-penicillamine in d-penicillamine and to check the enantiomeric purity of pharmaceutical formulations of d-penicillamine.

      Classification: 3d
      106 017
      Forced-flow planar liquid chromatographic techniques (after twenty-two years)
      E. TYIHÁK*, E. MINCSOVICS (*Plant Protection Institute, Hungarian Academy of Sciences, Herman O. Str. 15, P. O. Box 102, 1525 Budapest, Hungary; etyih@nki.hu)

      J. Planar Chromatogr. 23, 382-395 (2010). The paper summarizes progress in the main forced-flow planar liquid chromatographic (FFPLC) techniques taking into account one group of FFPLC used in practice, OPLC and rotation planar chromatography and another group like e. g. electrochromatographic techniques and shear-driven chromatography which show interesting results at an experimental level. Progress in FFPLC deals with the diversity of further instrumental developments and its basis, the instrument developments, determination of the role of the adsorbent layer in OPLC, challenges in the OPLC instrument development, and analytical and preparative applications of OPLC. Centrifugal layer or rotation planar chromatography is mentioned briefly. Two electrochromatographic techniques have been developed to accelerate the mobile phase flow, planar electrochromatography and planar dielectrochromatography using direct and alternating currents, respectively. One of the newest techniques among FFPLC is shear-driven chromatography in which the mobile phase is between two plates and is forced to flow above and inside the very thin adsorbent layer with shear-driven force generated by a moving plate. For the study of the biological activity of natural and synthetic compounds the planar adsorbent layer is advantageous, namely for in-vitro and in-vivo studies.

      Keywords: review
      Classification: 3d
      117 026
      Application of orthogonal pressurized planar electrochromatography to micropreparative separation of test dye mixture – preliminary results
      R. GAJOS, E. LOPACIUK, T. DZIDO* (*Department of Physical Chemistry, Chair of Chemistry, Medical University, Lublin, Poland, tadeusz.dzido@umlub.edu.pl)

      J. Planar Chromatogr. 29, 77-81 (2016). Orthogonal pressurized planar electrochromatography (OPPEC) of 4-(2-pyridylazo)resorcinol monosodium salt (1), patent blue (2), and azorubine (3) on RP-18W with 45 % methanol in buffer solution (pH 3.2, final concentration of buffer components in the mobile phase: 2.28 mmol/L of citric acid and 1.34 mmol/L of disodium hydrogen phosphate). The external pressure exerted on the adsorbent layer was set up as 15.0 bar. The polarization voltage applied to the electrodes was 1.5 kV, and the separation time was 150 min.

      Classification: 3d
      122 004
      Opportunities for 3D printed millifluidic platforms incorporating on-line sample handling and separation
      D. COCOVI-SOLBERG, P. WORSFOLD, M. MIRO* (*FI-TRACE Group, Department of Chemistry, University of the Balearic Islands, Carretera de Valldemossa km 7.5, 07122 Palma de Mallorca, Illes Balears, Spain, dj.cocovi.solberg@gmail.com)

      Trends Anal. Chem. 108, 13-22 (2018). Review of the potential of 3D printing for the manufacturing of millifluidic platforms for analytical chemistry. Key features of 3D printed devices incorporating chromatographic/electrophoretic separation was summarized, including photopolymer inkjet printing in a planar chromatography format for the separation of visible dyes and fluorescently tagged proteins.

      Keywords: HPTLC review
      Classification: 1, 3d