CBS 95, 10-13 (2005). HPTLC of five sulfonamides on silica gel with dichloroethane - methanol - 2-propanol - ammonia 25:5:5:1 in the twin trough chamber and ADC 2 with varied chamber saturation. Densitometric evaluation by absorbance measurement at 254 nm. Comparison of chamber saturation in conventional twin trough chamber and Automatic Development Chamber ADC 2 respectively. Reproducibility of Rf-values is better in ADC 2 due to higher quality in chamber saturation and less manual operations.

Isolation of the enantiomers. Chromatographia 68 (11-12), 1048-1051 (2008). TLC resolution of the enantiomers of the racemic drugs ketamine and lisinopril on silica gel with 1) ethyl acetate - methanol - water 3:1:1 and enantiomerically pure tartaric acid and (-)-mandelic acid as chiral impregnating reagents, for ketamine; and 2) acetonitrile - methanol - water - dichloromethane 14:2:2:1 with (+)-tartaric acid as the impregnating agent for lisinopril and using (+)-tartaric acid as mobile phase additive; and 3) acetonitrile - methanol - (+)-tartaric acid (0.5 % in water, pH 5) - acetic acid 70:10:11:7 which enabled successful resolution of the enantiomers of lisinopril. Investigation of the effects of temperature, pH, and the amount of chiral selector. Detection with iodine vapour. Isolation and identification of the separated enantiomers. The LOD was 0.25 and 0.27 µg for each enantiomer of ketamine with (+)-tartaric acid and (-)-mandelic acid, respectively, whereas 0.14 and 0.16 µg for each enantiomer of lisinopril with (+)-tartaric acid (both conditions) and (-)-mandelic acid, respectively.

J. of Chromatogr. A 1218 (33), 5693-5704 (2011) A micro-TLC platform for the fast analysis of low-molecular mass compounds from spirulina samples was developed. The target compounds were extracted with methanol, acetone or tetrahydrofuran. HPTLC on RP-18W with acetone - n-hexane 3:7 in an unsaturated chamber using a temperature controlled micro-planar chromatographic device based on a horizontal chamber. Detection under visible light before and after exposure to iodine vapor. Pictures of the chromatograms were acquired with an office scanner and digitalized. The quantitative data was analyzed using cluster analysis and principal components analysis. With this method it was possible to distinguish genuine spirulina and non-spirulina samples as well as fresh and expired commercial products.

J. Liq. Chromatogr. Relat. Technol. 39, 627-639 (2016). Review of the theory, materials and techniques of two-dimensional thin layer chromatography, including recent applications for the analysis of lipids, proteins and peptides, plant extracts and steroids on polyamide, cellulose, RP-18, cyano and diol phase._x000D_

R.E. Kaiser (Ed) "Proc. of the 3rd Int. Symp. on Instr.HPTLC, Wuerzburg", Bad Duerkheim 1985, 71-82. Description of the principle of automated multiple development technique and a fully automatic machine for the complexe developing process. A universal elution gradient is suitable for the separation of compounds differing widely in their polarity and those with unknown chromatographic behaviour. Several application examples.

International Lab. 15, (5) 22-3 (1985). 15 references.

The technique offers reproducible results of development.

J. Chromatogr. 404, 365-371 (1987). TLC, HPTLC, OPLC and UCLC of 8 main iridoid glycosides on silica with various solvent systems. Detection by spraying with 1% vanillin in sulfuric acid and densitometry at 420 nm. Comparison of the applicability of the techniques. UCLC = Ultra-micro-chamber Centrifugal Layer Chromatography.