External punishment function method.) (Chinese). Chinese J. Chromatogr. (Sepu) 13, 379-382 (1995). Proposal of a method for the optimization of the solvent systems of TLC, demonstrated by using sulfa drugs as example. Discussion of the effects of the composition and the proportion of the solvents.

Chromatographia 45, 369-372 (1997). Determination of apparent Rf values of a compound after successive development steps in incremental multiple development (IMD). Prediction of the migration and the zone width of investigated compounds in IMD with linearly and quadratically increasing development distances using naturally occurring compounds as model substances on different stationary phases.

J. Planar Chromatogr. 15, 144-146 (2002). Description of an improved method of two-dimensional development in TLC. The new feature is that before the second development the layer on the glass plate is divided into several zones and these zones can be developed with different mobile phases, instead with the same mobile phase. A mixture of 10 dyes was used to evaluate the technique; the results obtained were satisfactory.

Proc. Intern. Symp. on Planar Separations Plan. Chrom. 153-161 (2003). Optimum Performance Laminar Chromatography (OPLC) is a liquid chromatography technique using planar sorbent beds of low thickness (200 to 500 mm) in which the eluent is injected under pressure allowing parallel separation of samples. OPLC separation and isolation of a mixture of synthesized molecules and plant extract on planar sorbent beds (HTSorb) sealed on 4 edges on silica with e.g. 1)toluene or dichloromethane - ethyl acetate and on 2) RP-18 with a gradient water - acetonitrile 13:7 to 1:1 in 18 min or hexane - ethyl acetate from 99:1 to 1:99. The HTSorb were equilibrated before their use with the starting solution. Detection with optic fibers and Diode Array Detector (200-600 nm). New technique for chromatogram development.

J. Planar Chromatogr. 19, 109-114 (2006). Investigation of a new version of TLC with a closed adsorbent layer and an electroosmotic pump which was placed on the front of the plate and used to induce rapid movement of the mobile phase. Experimental evaluation of the new version of forced-flow TLC suggests that further elaboration of this version of TLC is appropriate.

J. AOAC Int. 91, 1196-1202 (2008). Thermostated micro HPTLC of Spirulina maxima dyes on RP18 with acetone - n-hexane 3:7 in a homemade temperature-controlled removable horizontal micro TLC chamber at 40 °C with chamber saturation. Detection by direct digital scan under visible light conditions. Experimental data indicated that under such conditions, with an office scanner used for chromatogram digitalization, spot quantification could be accurately performed within the analyte mass range of two orders of magnitide.

J. Liq. Chromatogr. Relat. Technol. 35, 294-307 (2012). A new chamber configuration, in which the feeding of the mobile phase to the TLC plate is carried out directly over the sorption layer of the plate, was proposed. The chamber is suitable for linear and circular TLC in ascending, horizontal, and descending modes of development.

J. Chromatogr. Sci. 54 (1), 64-69 (2016). Micro-TLC of 11 species of the Mentha genus and two finished pharmaceutical products in two-dimensional mode in normal (NP) and reversed-phase (RP) systems on cyano phase with non-aqueous eluents for NP-TLC and with mixtures of acetonitrile with water or methanol with water for RP-TLC. Optimization of one-dimensional systems was performed by determining RM values vs. composition of mobile phase dependencies for standards occurring in various Mentha sp. On the basis of these dependencies the most selective chromatographic systems for each run were chosen. Then most selective eluents were applied to optimize two-dimensional systems by creating Rf in NP systems vs. Rf in RP systems correlations. The best two-dimensional systems were chosen on the basis of R(2) values for Rf vs. Rf correlations (the lowest values of R(2) coefficients). Two-dimensional micro-TLC was used to separate phenolic compounds of the examined plant materials.