Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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      132 008
      In silico exploration of Serratia sp. BRL41 genome for detecting prodigiosin Biosynthetic Gene Cluster (BGC) and in vitro antimicrobial activity assessment of secreted prodigiosin
      Farhana BOBY*, N.H. BHUIYAN, B. KANTI SAHA, S. SANDHANI DEY, A. KUMAR SAHA, M. AL BASHERA, S. PROSAD MOULICK, F. JAHAN, A. UZ ZAMAN, S.F. CHOWDHURY, S.R. NASER, S. KHAN, M. HASAN SARKAR (*Bangladesh Council of Scientific and Industrial Research (BCSIR Laboratories), Dhaka, Bangladesh;

      PLoS ONE 18(11), e0294054 (2023). Sample was a pigment extracted with methanol from cell pellets of Serratia marcescens strain BRL41 (Yersiniaceae). TLC on silica gel with ethyl acetate. Detection under daylight. The pink pigment had the same hRF value (87) and colour as prodigiosin from standard strain ATCC-13880.



      Classification: 9, 23a
      131 002
      Bioassay-guided fractionation leads to the detection of cholic acid generated by the rare Thalassomonas sp.
      F. PHEIFFER, Y. K.-H. SCHNEIDER, E. H. HANSEN, J. HAMMER ANDERSEN, J. ISAKSSON, T. BUSCHE, C. RÜCKERT, J. KALINOWSKI, L. van ZYL, Marla TRINDADE* (*Institute for Microbial Biotechnology and Metagenomics, Department of Biotechnology, University of the Western Cape, Bellville, Cape Town, South Africa;

      Marine Drugs 21(1), 2 (2023). Samples were methanol extracts of cultivated marine bacteria Thalassomonas actiniarum, T. viridans and T. haliotis (Colwelliaceae),  as well as cholesterol, cholic acid, and deoxycholic acid as standards. TLC on silica gel with n-hexane – ethyl acetate – methanol – acetic acid 20:20:5:2. After drying at room temperature, visualization by spraying with phosphomolybdic acid (10 % in ethanol) and heating with a heat-gun. For isolation of cholic acid (hRF 80), present in all samples, preparative TLC on silica gel with the same mobile phase, the corresponding band was scraped off with a surgical blade and extracted with methanol overnight. The isolated cholic acid was identified by LC-MS.

      Classification: 13c, 13d
      130 035
      Solid phase extraction and simultaneous chromatographic quantification of some non-steroidal anti-inflammatory drug residues: an application in pharmaceutical industrial wastewater effluent
      M. KORASHY*, S. GAWAD, N. HASSAN, M. ABDELKAWY (*Quality Department, Pharmaoverseas Company, 2Pharmaceutical Chemistry Department, College of Pharmacy, Prince Sattam Bin Abdul-Aziz University, Kingdom of Saudi Arabia,

      Braz. J. Pharm. Sci. 58, e18691 (2022). HPTLC of paracetamol (1), diclofenac sodium (2), ibuprofen (3), and indomethacin (4) in wastewater effluents on silica gel with n-hexane - ethyl acetate - acetic acid 12:7:1. Quantitative determination by absorbance measurement at 254 nm. The hRF values for (1) to (4) were 18, 56, 69 and 44, respectively. Linearity was between 0.1 and 0.9 µg/zone for (1) to (4). Inter-day and intra-day precisions were below 1 % (n=3). Mean recovery was 99.7 % for (1), 99.8 % for (2), 99.7 % for (3) and 99.2 % for (4). 

      Classification: 37c
      130 047
      High‑performance thin‑layer chromatography in combination with an acetylcholinesterase‑inhibition bioassay with pre‑oxidation of organothiophosphates to determine neurotoxic effects in storm, waste, and surface water
      N. BAETZ, T. SCHMIDT, J. TUERK* (*Institute of Energy and Environmental Technology, Bliersheimer Str. 58 – 60, 47229 Duisburg, Germany,

      Anal. Bioanal. Chem. 414, 4167-4178 (2022). HPTLC of organothiophosphates malathion, parathion, and chlorpyrifos in storm, waste, and surface water on silica gel with an eluent mixture of cyclohexane, dichloromethane, and acetone in different proportions and with increasing migration distances. The plate was sprayed with n-bromosuccinimide to oxidize the organothiophosphates. AChE‑inhibition assay was performed by spraying with an AChE solution, followed by incubation at 37 °C for 5 min. Plates were sprayed with the substrate indoxyl acetate freshly prepared in methanol, followed by incubation for 45 min at room temperature. Detection at 670 nm using the fluorescence mode. 

      130 073
      Global distribution and potential risks of artificial sweeteners (ASs) with widespread contaminant in the environment: The latest advancements and future development
      X. WANG (Wang Xinglei), X. LIANG (LIang Xujun), X. GUO (Guo Xuetao)* (*College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, 712100, China,

      Trends Anal. Chem. 159, 116915 (2023). Review of analytical methodologies, distribution, ecotoxicity and removal of artificial sweeteners to determine the ecological and health risks that these substances may pose based on available data. The paper described analytical techniques, including HPTLC for the determination of artificial sweeteners in water samples.

      Classification: 37c
      130 074
      Recent advances in sampling and sample preparation for effect-directed environmental analysis
      S. HUANG (Huang Shuyao), M. FAN (Fan Mengge), N. WAWRYK, J. QIU (Qiu Junlang)*, X. YANG (Yang Xin), F. ZHU (Zhu Fang), G. OUYANG, X. LI (Li Xingfang) (*School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, 510006, China,

      Trends Anal. Chem. 154, 116654 (2022). Review of recent advances of sampling and sample preparation in effect-directed environmental analysis with a special focus on innovative approaches. The paper summarized sampling, enrichment and fractionation techniques for effect-directed analysis of water samples, including HPTLC methods for the analysis of potential toxicity contributors.

      Classification: 37c
      130 004
      Identification of acetylcholinesterase inhibitors in water by combining two-dimensional thin-layer chromatography and high-resolution mass spectrometry
      Lena STÜTZ*, W. SCHULZ, R. WINZENBACHER (*Laboratory for Operation Control and Research, Zweckverband Landeswasserversorgung, Langenau, Germany;

      J Chromatogr A, 1624, 461239 (2020). Samples were chemical standards of acetylcholinesterase (AChE) inhibitors (azamethiphos, caffeine, donepezil, galanthamine, methiocarb-sulfoxide, paraoxon-ethyl) and of neurotoxic compounds, as well as drinking or contaminated water samples enriched through solid phase extraction. HPTLC on spherical silica gel (pre-washed twice by 20 min immersion in isopropanol, heated 20 min at 120 °C before and after pre-washing with acetonitrile). First separation (preparative TLC) with automated multiple development (16 steps). Effect-directed analysis for AChE inhibitors by immersion (speed 5 cm/s, time 1 s) into enzyme solution, incubation 5 min at 37 °C and immersion into substrate solution (indoxyl acetate 2 % in methanol); visualization under UV 366 nm. Active zones from untreated layers were eluted through the oval head of a TLC-MS interface to a second plate for a second separation with a panel of other mobile phases. Bands of interest were eluted from the second layer with water through the oval elution head of the TLC-MS interface pump, into a RP18 liquid chromatography guard column, followed by a quadrupole time-of-flight mass spectrometer. Full scan mass spectra (m/z 100–1200) were recorded in negative and positive modes using electrospray ionization (and collision-induced dissociation for MS2). Among the water contaminants, lumichrome (riboflavin photolysis product), paraxanthine and linear alkylbenzene sulfonates were identified as AChE inhibitors.

      Classification: 3d, 4d, 4e, 22, 29b, 35d, 37c
      130 007
      Planar chromatography-bioassays for the parallel and sensitive detection of androgenicity, anti-androgenicity and cytotoxicity
      C. RIEGRAF, A.M. BELL, M. OHLIG, G. REIFFERSCHEID, S. BUCHINGER* (*Federal Institute of Hydrology, Koblenz, Germany;

      J Chromatogr A, 1684, 463582 (2022). Samples were concentrated filtrates of leachates of waste deposition sites, as well as testosterone, flutamide, bisphenol A (BPA) and nitroquinoline oxide (NQO) as standards. Automated Multiple Development on HPTLC silica gel (prewashed with methanol and dried 30 min at 110 °C) with 1) methanol up to 20 mm; 2A) chloroform – ethyl acetate –petroleum ether 11:4:5 or 2B) ethyl acetate – n-hexane 1:1 for flutamide and testosterone, up to 90 mm. Effect-directed analysis was performed by automated spraying 3 mL suspension of BJ1991 yeast (transfected Saccharomyces cerevisiae strain, pure for androgenic activity, with 50 ng/mL testosterone for anti-androgenic assay), followed by 20 h incubation at 30 °C in a closed chamber (90 % relative humidity), by 5 min drying under cold air stream, by spraying 2.5 mL MUG solution (4-methylumbelliferyl-galactopyranoside) and by 15 min incubation at 37 °C in an open chamber. Agonistic and antagonistic activities were detected qualitatively under UV 366 nm (light or dark blue bands, respectively, on blue background) and quantitatively documented using automated scanning at excitation wavelength 320 nm (deuterium lamp), with cut-off filter at 400 nm. Dose-response curves for model compounds were established by regression analysis. Anti-androgenic effective doses at 10 % were 28 ng/zone for flutamide and 20 ng/zone for BPA, without toxicity for the yeast. To exclude cytotoxicity where anti-androgenic activity was observed, the HPTLC layers (either without or after the spraying with MUG) were sprayed with 3 mL resazurin solution (0.01 % in water) and incubated 30 min at 30 °C and 90 % humidity. Cytotoxicity bands appeared as pink zones of resorufin on a colorless background (dihydroresorufin) under white light. Densitometric evaluation in absorption mode at 575 nm (under deuterium and halogen-tungsten lamps, no filter applied). NQO was cytotoxic at its lowest tested dose (1 ng/zone).

      Classification: 4b, 4e, 32d, 37c, 37d