60th Indian Pharmaceutical Congress PG-256 (2008). HPTLC of rosmarinic acid in Salvia officinalis on silica gel with toluene - ethyl acetate - formic acid 5:4:1. Quantitative determination by absorbance measurement at 328 nm. Linearity was 0.1-1.0 ng/mL, recovery was 99.4 %. The method was found suitable for routine quality control of the Salvia officinalis raw material.

J. Liq. Chromatogr. Relat. Technol. 34, 817-828 (2011). HPTLC of diclofenac (1) and ibuprofen (2) in aqueous environmental samples, on cyano phase with dichloromethane - methanol - cyclohexane 19:1:8. Detection by dipping into a Vibrio fisheri bacteria suspension for 3 sec. Then a glass plate was placed on top of the layer and a light-sensitive camera was used to measure the luminescence for 1 to 10 min. Linearity was between 10 and 2000 ng/zone. Limits of detection and quantification were 89 and 129 ng/band for (1), and 20 and 26 ng for (2). The coupling of HPTLC with a luminescent bacteria assay is suitable to determine drugs in aqueous environmental samples.

J. Planar Chromatogr. 26, 395-401 (2013). HPTLC of 17alpha-ethinylestradiol (1), 17beta-estradiol (2) and estrone (3) on silica gel with chloroform - acetone - petroleum 11:4:5. The method was combined with the Yeast Estrogen Screen for the detection of estrogenic effects in sediment extracts of a river. Quantitative determination by absorbance measurement at 320 nm. The hRf values of compounds (1) to (3) were 74, 61 and 84, respectively.LOD and LOQ were calculated for the estrogenic model compound (1): LOD was 0.46 pg without prior development of the TLC plate and 0.48 pg after development, LOQ was 0.8 pg without development and 1.6 pg after development.

J. Planar Chromatogr. 28, 162-166 (2015). HPTLC of (1) sucrose, (2) glucose, (3) fructose, (4) arabinose, (5) cellobiose, (6) maltose, (7) mannose, (8) melezitose, (9) melibiose, (10) raffinose, (11) rhamnose, (12) stachyose, (13) trehalose and (14) xylose on silica gel with n-butanol - i-propanol - boric acid solution - formic acid 50:130:55:1 to a migration distance of 60 mm. Detection under white light after immersion into aniline diphenylamine o-phosphoric acid reagent and heating at 110 °C for 10 min. Qualitative detections were performed under white light and by absorption measurement at 380 nm. A total of 131 samples were analyzed with regard to their individual sugar composition and classified according to their sugar ratios.

J. Liq. Chromatogr. Relat. Technol. 40, 226-238 (2017). Review of the following topics for the period of November 1, 2014 to November 1, 2016: two-dimensional (2D) TLC and multidimensional TLC methods for effective separations of complex pesticide mixtures; ultraviolet (UV) spectrometry, HPLC, potentiometry, calorimetry, titrimetry, and HPTLC methods for determination of the nitroimidazole fungicides omidazole and tinidazole; and chromatographic methods for identification of plant protection products (formulations), as well as book chapters reviewed on pesticide analysis by instrumental TLC, particularly using automated multiple development.

Application to real samples. J. High Resol. Chromatogr. 8, 135-139 (1985). Separation of inorganic and organic mercury dithizonates on silica (after conditioning with 5 M NH3) with hexane - acetone 85:15. Quantification by densitometry at 470 nm.

Investigations on the analysis of multi-valent phenolic compounds on TLC sorption layers impregnated with ammonium molybdate. Z. Anal. Chem. 330, 531 (1988). TLC of 1,2-, 1,3-, 1,4-dihydroxybenzene with toluene - acetone - chloroform 40:35:25 and of 1,2,3-, 1,3,5-, 1,2,4-trihydroxybenzene with benzene - acetone 70:30 on ammonium molybdate impregnated silica. Detection limits 0.6-1 µg.

J. Planar Chromatogr. 2, 290-296 (1989). HPTLC separation of 15 urea herbicides on alumina with dichloromethane, on silica C-18 with methanol - water 75:25, and on silica with chloroform - diisopropylether 9:1, and diisopropyl ether - isobutyl methyl ketone 8:2. Detection by fluorescence quenching and/or reflectance resp. absorbance scanning.