J. Liq. Chromatogr. Relat. Technol. 39, 322-324 (2016). HPTLC of neutral (1) and polar lipids (2) in Biomphalaria glabrata snails on silica gel with petroleum ether – diethyl ether – glacial acetic acid 80:20:1 for (1) and chloroform – methanol – water 130:50:9 for (2). Detection of neutral lipids by spraying with 5 % phosphomolybdic acid in absolute ethanol and polar lipids using 10 % copper (II) sulfate in 8 % phosphoric acid.

J. Planar Chromatogr. 31, 72-78 (2018). HPTLC of equol in ethanolic cattle manure extract on RP-18 with n-hexane – ethyl – acetate – acetone 9:3:2. Detection by planar yeast estrogen screening (pYES) by dipping into a yeast suspension, followed by incubation at 30 °C for 4 h. After incubation, the plate was dried in a 37 °C incubator for 15 min and dipped into the combined reaction buffer followed by incubation at 37 °C for 60 min and 90 % relative humidity. The combined reaction buffer was prepared by mixing 20 mL of buffer C (5.3 g of sodium phosphate dibasic and 0.4 g of potassium chloride were dissolved in about 490 mL water, the solution was adjusted with sodium hydroxide to pH 13, 0.5 g of benzalkonium chloride were added and the mixture was filled up to 500 mL) and 0.2 mL of a freshly prepared X-Gal solution (0.05 g/mL X-Gal in DMSO). Fluorescence evaluation under UV 366 nm. The hRF value for equol was 47.

Detection by their inhibition of esterase of rat or pig.

Determination of organo-phosphorus ester in food products originating from animals.) (Hungarian). MSZ (Hungarian Norm), 14475/44, 15 (1988). TLC of trichlorphon, dimetoat, dichlorphos, diazinon, malathion, methylparathion, parathion and bromofos on silica with benzene - acetone 95:5, hexane - acetone 6:4 or 85:15. Detection by enzymatic method (colinesterase + ß-naftilacetate). Detection limit 1-10 ng organo-phosphorus ester.

J. Planar Chromatogr. 2, 262-267, (1989). Preconcentration procedure and HPTLC separation of 13 pesticides (phenylureas, carbamates, triazines, and other N-heterocyclic compounds) using HPTLC, development by a 30-step gradient (methanol - chloroform) using the AMD technique; detection by measuring the UV reflectance at 6 wavelengths ( = 190, 220, 240, 260, 280, and 300 nm). CV ± 2,2% at a concentration range between 20 and 500 ng.

J. Planar Chromatogr. 3, 504-510 (1990). Multiple and stepwise development combined with gradient elution as a highly suitable method for the systematic determination of crop protection agents. The migration distance is highly reproducible (on the same HPTLC plate and on different ones). Screening and confirmation gradients coupled with reflectance spectroscopy (multiwavelength scanning) and postchromatographic, microchemical derivatization make it possible to detect crop protection agents in drinking, table, and ground waters. At least 100 substances can be checked for their presence on one HPTLC plate. Example: HPTLC separation of 21 crop protection agents in ground and drinking water with AMD. Two 23-step gradients (33 runs) based on acetonitrile, dichloromethane, hexane, formic acid, NH3 25% and on tert buthyl methyl ether, acetonitrile, hexane, formic acid, NH3. Multiwavelength scanning by adsorbance at 190, 220, 240, 260, 280, and 300 nm

J. Planar Chromatogr. 7, 372-375 (1994). TLC of the technical products from the synthesis of the title substance on silica with methanol - pyridine - formamide 80:15:5. Detection by inhibition of choline esterase: spraying the plates first with enzyme solution, and after incubation for 30 min at room temperature, with the mixed substrate - DTNB solution. After use of ninhydrin, drying for 10 min and heating at 120 °C red and reddish-blue spots appear on a pink background.

J. Chromatogr. A 710, 117-128 (1995). A review with 61 references including PC and TLC methods. Discussion of the advantages of each.