J. Planar Chromatogr. 9, 450-455 (1996). Analytical TLC of aminoarenes (aminonaphthalene, -phenylnaphthalene, -pyrene, -fluoroanthene, -anthracene, -phenanthrene, -quinoline, isoquinoline, -chrysene) on RP-18 with acetonitrile - water 9:1; semipreparative TLC on aluminium oxide with dichloromethane - hexane - ether 1:1:1. Visualization under UV 254 and 366 nm.

CBS 94, 2-4 (2005). HPTLC-AMD of four pharmaceuticals and extracts of surface water on silica gel prewashed with 2-propanol (immersion for 24 h) with a 25-step gradient based on acetonitrile - formic acid - dichloromethane. Luminographic detection at ng-level by immersion of the developed HPTLC plates into Vibrio fischeri bacteria suspension. Visual evaluation with CCD-camera, exposure time 40 s, inversion and scaling of exposure in pseudocolors. To remove matrix (humic acids) from surface water samples size exclusion chromatography is recommended.

Acta Chrom. 13, 117-134 (2003). HPTLC of 11 heavy metal cations on silica gel F254 with pure organic, mixed organic and mixed aqueous – organic mobile phases. Mobile phases such as methanol – dimethylamine 4:1 and methanol – dimethylamine – formic acid 4:4:1 were found most suitable for rapid separation and identification of mixtures of Cr6+ and Cr3+ and of Cr6+, Ni2+ and Co2+, respectively. Detection of Cd2+, Ag+, Pb2+, Tl+, Bi3+ and Hg2+ by spraying with yellow ammonium sulphide reagent, of VO2+ with a 1 % aqueous solution of potassium ferrocyanide, of Ni2+ and Co2+ with dimethylglyoxime (0.2 % in ammonia), of Cr6+ with a saturated alcoholic solution of AgNO3 and of Cr3+ with a 1 % alizarin red in methanol. The effect of impurities such as inorganic ions, phenols, and surfactants on the separation of Cr6+ and Cr3+ was examined. The proposed method was successfully used for analysis of industrial wastewater samples.

J. Planar Chromatogr. 22, 65-71 (2009). Presentation of two simple and rapid HPTLC methods for early detection of the effects of herbicides using two different groups of plant biomarkers, which were developed as field tests (Herbicide Weed Response test - HWR-Test). Phytochemical changes can be detected before any morphological changes are visible on the plants. These changes are defined as biomarkers and can be detected by HPTLC-screening. After overall identification of the phytochemical biomarker pattern, two different biomarker groups, carbohydrates and amino acids, were detected using modified reagents for color reactions. Evaluation under daylight and videodensitometric analysis of digital images by VideoScan software. The screening method was previously described [H. W. Ravn, M. Hjorth, L. Lauridsen, P. Kudsk, S. K. Mathiassen, L. Mondolot, Bull. Environ. Contam. Toxicol. 75, 236-245 (2005)].

Journal of Environmental Science and Health, Part B 46, 557-568 (2011). Review on techniques and applications of TLC and HPTLC for separation, detection, qualitative and quantitative determination and preparative isolation of pesticides. Covered are sample preparation techniques, stationary phases, sample application, mobile phases, development methods using different chambers, detection under UV or by derivatization with various reagents, identification based on hRf values or by online HPTLC-MS, quantification by scanning densitometry or videodensitometry, preparative layer chromatography and thin-layer radiochromatography. Various applications are described. In the review period especially forensic analyses of human and animal samples for pesticides were numerous. The identification and quantification of components from plant extracts with pesticide activity is also reviewed and it is expected that this area will be especially active in the future given the large amount of ongoing worldwide research on phytochemical compounds.

J. Liq. Chromatogr. Relat. Technol. 36, 2378-2386 (2013). 2D-HPTLC of testosterone (1), progesterone (2), hydrocortison (3), estriol (4), ethinylestradiol (5), sitosterol (6), estrone (7), prednisolone (8), estradiol (9) and norethindrone (10) in waste water on cyanopropyl silica gel with dichloromethane - methanol - cyclohexane 19:1:12 in the first direction and water - acetonitrile - ethanol - dioxane 8:2:1:1+1 drop ammonia in the second direction. Detection by heating at 110 °C for 1 min followed by dipping into a mixture of sulfuric acid 98 % - water 1:49 and heating at 110°C for 10 min. Quantitative determination by absorbance measurement at 366 nm. The hRf values for the first and second direction were 80 and 5 for (1), 72 and 9 for (2), 32 and 21 for (3), 8 and 31 for (4), 31 and 11 for (5), 80 and 0 for (6), 44 and 12 for (7), 12 and 34 for (8), 71 and 1 for (9) and 62 and 16 for (10). LOD and LOQ for 17alpha-ethinylestradiol were 1 and 2 ng/zone, respectively.

J. Planar Chromatogr. 28, 190-204 (2015). The analysis of multiple pesticide residues in environmental samples is often impossible in a single-step process. Comprehensive 2D planar chromatography on mono and bilayers, coupled-layer chromatography, combination of multidimensional planar chromatographic techniques, hyphenated methods are proved to be suitable.

J. Planar Chromatogr. 29, 361-365 (2016). Analysis of phenols in bio-oil from agricultural wastes obtained during fractionation. Column chromatography (CC) on silica gel in a glass column (i.d. 1 cm, 50 cm length) filled with n-hexane. The concentrated organic phase was mixed with silica gel 1:1, loaded onto the column and successively eluted with dichloromethane – acetone 20:1, ethyl acetate, and methanol. 24 eluate fractions were obtained during the fractionation using CC, 12 with dichloromethane – acetone mixture, 5 with ethyl acetate, and 7 with methanol. Phenolic compounds were present only in the dichloromethane – acetone mixture fraction which was therefore selected as the proper eluent to obtain all the phenolic fractions dissolved. To monitor the fractionation, TLC of the eluates on silica gel in saturated chambers (mobile phase not specified) to a distance of 4 cm. Qualitative identification under UV 254 nm and by exposure to iodine vapor. The hRf values of the standard solutions of phenol, cresol and guaiacolin dichlorlomethane were 66, 68 and 78, respectively. Eluate fractions were further analyzed by GC–FID and GC–MS.