Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      130 004
      Identification of acetylcholinesterase inhibitors in water by combining two-dimensional thin-layer chromatography and high-resolution mass spectrometry
      Lena STÜTZ*, W. SCHULZ, R. WINZENBACHER (*Laboratory for Operation Control and Research, Zweckverband Landeswasserversorgung, Langenau, Germany; stuetz.l@lw-online.de)

      J Chromatogr A, 1624, 461239 (2020). Samples were chemical standards of acetylcholinesterase (AChE) inhibitors (azamethiphos, caffeine, donepezil, galanthamine, methiocarb-sulfoxide, paraoxon-ethyl) and of neurotoxic compounds, as well as drinking or contaminated water samples enriched through solid phase extraction. HPTLC on spherical silica gel (pre-washed twice by 20 min immersion in isopropanol, heated 20 min at 120 °C before and after pre-washing with acetonitrile). First separation (preparative TLC) with automated multiple development (16 steps). Effect-directed analysis for AChE inhibitors by immersion (speed 5 cm/s, time 1 s) into enzyme solution, incubation 5 min at 37 °C and immersion into substrate solution (indoxyl acetate 2 % in methanol); visualization under UV 366 nm. Active zones from untreated layers were eluted through the oval head of a TLC-MS interface to a second plate for a second separation with a panel of other mobile phases. Bands of interest were eluted from the second layer with water through the oval elution head of the TLC-MS interface pump, into a RP18 liquid chromatography guard column, followed by a quadrupole time-of-flight mass spectrometer. Full scan mass spectra (m/z 100–1200) were recorded in negative and positive modes using electrospray ionization (and collision-induced dissociation for MS2). Among the water contaminants, lumichrome (riboflavin photolysis product), paraxanthine and linear alkylbenzene sulfonates were identified as AChE inhibitors.

      Classification: 3d, 4d, 4e, 22, 29b, 35d, 37c
      130 007
      Planar chromatography-bioassays for the parallel and sensitive detection of androgenicity, anti-androgenicity and cytotoxicity
      C. RIEGRAF, A.M. BELL, M. OHLIG, G. REIFFERSCHEID, S. BUCHINGER* (*Federal Institute of Hydrology, Koblenz, Germany; buchinger@bafg.de)

      J Chromatogr A, 1684, 463582 (2022). Samples were concentrated filtrates of leachates of waste deposition sites, as well as testosterone, flutamide, bisphenol A (BPA) and nitroquinoline oxide (NQO) as standards. Automated Multiple Development on HPTLC silica gel (prewashed with methanol and dried 30 min at 110 °C) with 1) methanol up to 20 mm; 2A) chloroform – ethyl acetate –petroleum ether 11:4:5 or 2B) ethyl acetate – n-hexane 1:1 for flutamide and testosterone, up to 90 mm. Effect-directed analysis was performed by automated spraying 3 mL suspension of BJ1991 yeast (transfected Saccharomyces cerevisiae strain, pure for androgenic activity, with 50 ng/mL testosterone for anti-androgenic assay), followed by 20 h incubation at 30 °C in a closed chamber (90 % relative humidity), by 5 min drying under cold air stream, by spraying 2.5 mL MUG solution (4-methylumbelliferyl-galactopyranoside) and by 15 min incubation at 37 °C in an open chamber. Agonistic and antagonistic activities were detected qualitatively under UV 366 nm (light or dark blue bands, respectively, on blue background) and quantitatively documented using automated scanning at excitation wavelength 320 nm (deuterium lamp), with cut-off filter at 400 nm. Dose-response curves for model compounds were established by regression analysis. Anti-androgenic effective doses at 10 % were 28 ng/zone for flutamide and 20 ng/zone for BPA, without toxicity for the yeast. To exclude cytotoxicity where anti-androgenic activity was observed, the HPTLC layers (either without or after the spraying with MUG) were sprayed with 3 mL resazurin solution (0.01 % in water) and incubated 30 min at 30 °C and 90 % humidity. Cytotoxicity bands appeared as pink zones of resorufin on a colorless background (dihydroresorufin) under white light. Densitometric evaluation in absorption mode at 575 nm (under deuterium and halogen-tungsten lamps, no filter applied). NQO was cytotoxic at its lowest tested dose (1 ng/zone).

      Classification: 4b, 4e, 32d, 37c, 37d
      124 063
      Determination of anthelmintic pharmaceuticals in wastewater by solid-phase extraction and thin-layer chromatography
      Dragana PAVLOVIC*, T. KRALIKEVIC, R. PAVIC, J. MRDA (*Department of Analytical Chemistry; and T. Gazivoda Kraljevic, Department of Organic Chemistry, Faculty of Chemical Engineering and Technology, University of Zagreb, Marulicev trg 19, 10000 Zagreb, Croatia, dmutavdz@fkit.hr)

      J. Planar Chromatogr. 32, 421-429 (2019). HPTLC of levamisole (1), pyrantel pamoate (2), albendazole (3), and febantel (4) on silica gel with dichloromethane - methanol - formic acid 18:1:1. Quantitative determination by absorbance measurement at 254 nm. The hRF values for (1) to (4) were 10, 15, 47 and 79, respectively. Linearity was between 600 and 1500 µg/L for (1), 400 and 1000 µg/L for (2), 200 and 1000 µg/L for (3) and (4). The obtained RSD values were in the range from 7.0 % to 8.8 % for intra-day precision and from 8.1 % to 13.1 % for inter-day precision (n=3). The LOD and LOQ were 300 and 600 µg/L for (1), 200 and 400 µg/L for (2) and 100 and 200 µg/L for (3) and (4), respectively. Recovery rate was above 95 % for (1) to (4).

      Classification: 37c
      123 029
      Identification of genotoxic transformation products by effect-directed analysis with High-Performance Thin-Layer Chromatography and non-target screening
      Lena STÜTZ*, Patricia LEITNER, W. SCHULZ, R. WINZENBACHER (*Laboratory for Operation Control and Research, Zweckverband Landeswasserversorgung, Am Spitzigen Berg 1, 89129 Langenau, Germany, Stuetz.L@lw-online.de)

      J. Planar Chromatogr. 32, 173-182 (2019). HPTLC of chlorinated metformin samples with genotoxic effect obtained by umu assay (Salmonella typhimurium TA1535/pSK1002 Assay) on silica gel in a gradient development with methanol - formic acid 2000:1, dichloromethane and n-hexane. A TLC-MS interface was used for further analysis by LC-high-resolution mass spectrometry. 

      Classification: 32a, 37c
      100 185
      Factors affecting the separation of phthalate esters, and their analysis, by HPTLC
      R. ZHANG* (Zhang Rong), Y. YUE (Yue Yongde), R. HUA (Hua Rimao), W. YAN (Yan Wen) (*Recourses and Environment College of Anhui Agricultural University, Agri-food Security Key Lab of Anhui Province, No. 130, Changjiang West Road, Hefei, China; z_rong163@163.com)

      J. Planar Chromatogr. 20, 321-326 (2007). Investigation of factors affecting the separation, including the use of different stationary and mobile phases, different methods of development, humidity, and chamber saturation. TLC and HPTLC of dimethyl, diethyl, di-n-butyl, and bis-(ethylhexyl) phthalate on silica gel, prewashed with chloroform - methanol 1:1 or the mobile phase, in horizontal chambers, Vario chambers, and twin-trough chambers with 12 different mobile phases. Best separations were achieved with hexane - acetone 4:1 or hexane - toluene - ethyl acetate 9:8:3. Densitometric evaluation at 220 nm.

      Classification: 37c
      120 061
      Planar yeast estrogen screen with resorufin-?-D-galactopyranoside as substrate
      D. SCHICK, W. SCHWACK* (*Inst. of Food Chem., Univ. of Hohenheim, Garbenstraße 28, 70599 Stuttgart, Germany, wolfgang.schwack@uni-hohenheim.de)

      J. Chromatogr. A 1497, 155-163 (2017). Development of a planar yeast estrogen screen for the determination of estrogen active compounds. Introduction of resorufin-β-D-galactopyranoside, providing the orange fluorescing resorufin after enzymatic cleavage, as the planar yeast estrogen screen (pYES) substrate to determine estrogen active compounds (EAC). For samples containing blue fluorescent components, this substance is better suited than the generally employed substrate 4-methylumbelliferyl-β-D-galactopyranoside, which delivered blue fluorescing 4-methylumbelliferone after enzymatic cleavage by the YES reporter β-D-galactosidase. The mean LOD and LOQ was 3.5 and 6.5 pg/zone for 17β-estradiol (1) and 17α-ethinylestradiol (2), respectively; recoveries were close to 100% for (1) and (2) from spiked water samples in a concentration range of 2–20 ng/L.

      Classification: 13b, 37c
      75 111
      Application of TLC and GC-MS to the identification of azaarenes in sewage sludges
      K. TYRPIEN, D. BODZEK*, B. JANOSZKA, (Silesian Med. Acad., Fac. of Med., Dept. of Chem., 41-808 Zabrze, H. Jordana Street 19, Poland)

      J. Planar Chromatogr. 8, 75-77 (1995). TLC of azaarenes (i.a. acridine, benzo(h)quinoline, benzo(f)quinoline, phenanthridine, azafluorene) on silica with dichloromethane - methanol 20:1. Detection under UV 254 and 265 nm, followed by spraying with specific reagents for detection of functional groups. GC-MS after elution with dichloromethane - methanol 1:1.

      Classification: 23e, 37c
      97 162
      Solid-phase extraction and TLC quantification of enrofloxacin, oxytetracycline, and trimethoprim in wastewater
      Danijela Asperger*, D. Mutavdzic, S. Babic, A. J. M. Horvat, M. Kastelan-Macan (*Faculty of Chemical Engineering and Technology, Laboratory of Analytical Chemistry, Marulicev Trg 19, 10000 Zagreb, Croatia)

      J. Planar Chromatogr. 19, 129-134 (2006). HPTLC of enrofloxacin, oxytetracycline, and trimethoprim on cyano phases with 0.5 M oxalic acid - methanol (5:5; 6:4; 7:3; 8:2). Detection under UV light at 254 nm. Quantitation by videodensitometry at 254 nm.

      Classification: 37c