Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      130 007
      Planar chromatography-bioassays for the parallel and sensitive detection of androgenicity, anti-androgenicity and cytotoxicity
      C. RIEGRAF, A.M. BELL, M. OHLIG, G. REIFFERSCHEID, S. BUCHINGER* (*Federal Institute of Hydrology, Koblenz, Germany;

      J Chromatogr A, 1684, 463582 (2022). Samples were concentrated filtrates of leachates of waste deposition sites, as well as testosterone, flutamide, bisphenol A (BPA) and nitroquinoline oxide (NQO) as standards. Automated Multiple Development on HPTLC silica gel (prewashed with methanol and dried 30 min at 110 °C) with 1) methanol up to 20 mm; 2A) chloroform – ethyl acetate –petroleum ether 11:4:5 or 2B) ethyl acetate – n-hexane 1:1 for flutamide and testosterone, up to 90 mm. Effect-directed analysis was performed by automated spraying 3 mL suspension of BJ1991 yeast (transfected Saccharomyces cerevisiae strain, pure for androgenic activity, with 50 ng/mL testosterone for anti-androgenic assay), followed by 20 h incubation at 30 °C in a closed chamber (90 % relative humidity), by 5 min drying under cold air stream, by spraying 2.5 mL MUG solution (4-methylumbelliferyl-galactopyranoside) and by 15 min incubation at 37 °C in an open chamber. Agonistic and antagonistic activities were detected qualitatively under UV 366 nm (light or dark blue bands, respectively, on blue background) and quantitatively documented using automated scanning at excitation wavelength 320 nm (deuterium lamp), with cut-off filter at 400 nm. Dose-response curves for model compounds were established by regression analysis. Anti-androgenic effective doses at 10 % were 28 ng/zone for flutamide and 20 ng/zone for BPA, without toxicity for the yeast. To exclude cytotoxicity where anti-androgenic activity was observed, the HPTLC layers (either without or after the spraying with MUG) were sprayed with 3 mL resazurin solution (0.01 % in water) and incubated 30 min at 30 °C and 90 % humidity. Cytotoxicity bands appeared as pink zones of resorufin on a colorless background (dihydroresorufin) under white light. Densitometric evaluation in absorption mode at 575 nm (under deuterium and halogen-tungsten lamps, no filter applied). NQO was cytotoxic at its lowest tested dose (1 ng/zone).

      Classification: 4b, 4e, 32d, 37c, 37d
      120 025
      Thin-layer chromatography coupled with high performance liquid chromatography for determining tetrabromobisphenol A/S and their derivatives in soils
      A. LIU, Z. SHEN, Y. TIAN, R. SHI, Y. LIU, Z. ZHAO*, M. XIAN (*CAS Key Lab. of Biobased Materials, Qingdao Inst. of Bioenergy & Bioprocess Technol., Chinese Acad. of Sci., Qingdao, 266101, China,

      J. Chromatogr. A 1526, 151-156 (2017). Development of a rapid and cost-effective method for determining brominated flame retardants (BFR) such as tetrabromobisphenol A/S (TBBPA/S) and their derivatives in soils, including TBBPA, TBBPA bis(allyl ether) (TBBPA-BAE), TBBPA bis(2,3-dibromopropyl ether) (TBBPA-BDBPE), TBBPS bis(allyl ether) (TBBPS-BAE) and TBBPS bis(2,3-dibromopropyl ether) (TBBPS-BDBPE), based on TLC sample pre-treatment coupled with HPLC-DAD at UV 214 nm. The LODs and LOQs were between 0.023-0.087 μg/g dw and 0.076-0.29 μg/g dw, respectively. The recoveries were between 41-108 % and both % RSD of repeatability and intermediate precision were < 11 %. The method showed a good performance for analyzing natural soil samples collected from BFRs industrial park, suggesting its great application potential for monitoring environmental TBBPA/S and their derivatives.

      Classification: 4d, 7, 37d
      69 224
      The behaviour of some benzene derivatives on thin layers of aluminium oxide - comparison with plain and Fe(III) impregnated silica gel
      S. KVEDER, S. ISKRIC*, N. ZAMBELI, O. HADZIJA, (*The Ruder Boskovic Institute, Zagreb, Croatia, Yugoslavia)

      J. Liquid Chromatogr. 14, 3277-3282 (1991). TLC of some carboxy and hydroxy benzene derivatives on aluminium oxide and Fe(III) impregnated silica with water. Detection by spraying with 0.05% ethanolic bromocresol green indicator reagent and by inspection under UV light. Comparison of the behavior of both systems.

      Classification: 37d
      73 174
      Mobility of cadmium as influenced by soil properties, studied by soil thin-layer chromatography
      M. SANCHEZ-CAMAZANO*, M.J. SANCHEZ-MARTIN, (*Inst. de Recursos Not. Y Agrobiol., CSIC, Cordel de Merinos 40 - 52, Apdo 257, E-37071, Spain)

      J. Chromatogr. 643, 357-362 (1993). Study of the mobility of cadmium in various natural soils by employing TLC techniques. Investigation of the correlation between Rf and pH value. Discussion of the significance of soil properties to the mobility of cadmium waters from industrial mining and farming provenience.

      Classification: 37d
      78 038
      Application of TLC and GC-MS to the identification of amino-PAHs in sewage sludges
      B. JANOZKA, K. TYRPIEN, D. BODZEK*. (Silesian Med. Acad., Fac. of Med., Dept. of Chem., H. Jordana Street 19, 41-808 Zabrze, Poland)

      J. Planar Chromatogr. 9, 450-455 (1996). Analytical TLC of aminoarenes (aminonaphthalene, -phenylnaphthalene, -pyrene, -fluoroanthene, -anthracene, -phenanthrene, -quinoline, isoquinoline, -chrysene) on RP-18 with acetonitrile - water 9:1; semipreparative TLC on aluminium oxide with dichloromethane - hexane - ether 1:1:1. Visualization under UV 254 and 366 nm.

      Classification: 37d
      79 247
      Study of the effect of exogenous organic matter on the mobility of pesticides in soils using soil thin-layer chromatography
      M. SANCHEZ-CAMAZANO*, M.J. SANCHEZ-MARTIN, E. POVEDA, E. IGLESIAS-JIMENEZ, (*Inst. Rec. Nat. y Agr., CSIC, Cordel Merinas 40-52, E-37071 Salamanca, Spain)

      J. Chromatogr. A 754, 279-284 (1996). Study of the effect of soil amendment using urban compost, agricultural organic amendments and surfactants on the mobility of diazinon and linuron by soil TLC on soil plate with water. 14C pesticide detected by radiography. Discussion of the modifications in Rf values due to the addition of the amendments, and of the correlation between the Rf values and the content of total organic carbon in the amended soils, and of the mechanism of the pesticide mobility.

      Classification: 29, 37d
      85 149
      A simplified HPTLC screening method for the estimation of the PAH content in soil samples
      H. KUTSCH*, U. SCHOEN, (*Catholic Univ. Eichstätt, Phys. Geogr., Landscape Ecology, Geolab., Ostenstrasse 18, D-85072 Eichstätt, Germany)

      Fresenius J. Anal. Chem. 367, 279-283 (2000). HPTLC of a EPA-PAH16 environmental standard and B[a]P, DB[ah]A, B[ghi]P, In[1,2,3-cd]P, Ant/Flu, B[b]F, B[k]F on silica gel RP-18 with ethanol - dichloromethane 1:1, mixed immediately before the run. Visualization under UV 366 nm. - Achievement of a deliberately incomplete separation of the visual fluorescing fraction of PAH and the total EPA-PAH16 content into a few fingerprint-like compressed bands within a determined "PAH window".

      Classification: 37d
      85 150
      Extraction and quantification of benzo[a]pyrene by reversed phase thin layer chromatography
      D.R. NEMERGUT, R.M. JOHNSON, K.G. WUNCH*, J.W. BENETT, (Dept. of Cell and Molecular Biol., Tulane Univ., 2000 Percival Sterne Hall, New Orleans, LA 70118, USA)

      J. Liqu. Chromatogr. 23, 579-586 (2000). HPTLC of benzo[a]pyrene on hydrocarbon impregnated RP-18 plates with methanol - acetonitrile 1:1. Quantification by densitometry at 370 nm.

      Classification: 5b, 37d