Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      131 007
      Principal component analysis and DoE-Based AQbD Approach to Multipurpose HPTLC method for synchronous estimation of multiple FDCs of metformin HCl, repaglinide, glibenclamide and pioglitazone HCl
      P. PRAJAPATI*, K. RADADIYA, S. SHAH (*Department of Quality Assurance, Maliba Pharmacy College, Uka Tarsadia University, Tarsadi, Gujarat, India;

      J Chrom Sci, bmad055 (2022). Standards of antiglycemic drugs were metformin hydrochloride (S1, a biguanide), glibenclamide (S2 = glyburide, a sulfonylurea), pioglitazone hydrochloride (S3, a thiazolidinedione), repaglinide (S4, a glinide). Samples were methanolic solutions of commercial tablets of S1 with each of the other molecules. The following method was developed by a software-assisted AQbD approach (analytical quality by design): (1) Several TLC separations were tried with toluene together with other solvents and with acidic or basic modifiers, with also variations of 24 method or instrumental parameters. (2) Principal component analysis (PCA) was performed in order to identify two principal components (PCs) responsible for 98 % of the observed variations: namely, resolution and tailing factor. Three critical method parameters (CMPs) had a statistically significant impact on the PCs: mobile phase (MP) composition, ammonium acetate concentration in MP, and saturation time. (3) To optimize these CMPs, the Box–Behnken design was implemented in 15 software-proposed experiments; the impacts of the 3 CMPs on the 2 PCs were evaluated by ANOVA, multiple regression analysis, and 2D and 3D contour plots. (4) The optimal CMPs ranges were determined by defining a MODR (method operable design region) on the superposed contour plots, and one TLC condition was selected as analytical control point.
      TLC on silica gel pre-washed with 10 mL methanol, dried and activated 10 min at 100° C. Separation with toluene – ethyl acetate – methanolic solution of 4 % ammonium acetate 7:7:6 after 15 min pre-saturation with 35 % relative humidity. Absorption emasurement at UV 254 nm. The hRF values were 13 for S1, 72 for S2, 82 for S3, 38 for S4. LOQ were 263, 387, 73 and 35 ng/zone, respectively. Linearity range was 25–75 µg/zone for S1, 100–300 ng/zone for S2 and S4, 750–2250 ng/zone for S3. Intermediate precision was below 2 %. For accuracy tests, recovery rates were between 97.6–101.4 %.

      Classification: 2e, 5c, 7, 8b, 17a, 17c, 23d, 23e, 24, 32a
      131 004
      Simultaneous determination of montelukast sodium and loratadine by eco-friendly densitometry and spectrophotometric methods
      Shimaa A. MAHMOUD*, A.M. EL-KOSASY, F.A. FOUAD
      (*Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt;

      J Chrom Sci, bmad025 (2023). Standards (separated and mixed) were montelukast sodium (MKT) and loratadine (LRT). Samples were methanolic solutions of commercial tablets, and purified blood plasma as biological fluid, from patients taking MKT or LRT as oral treatment. TLC on silica gel with ethyl acetate – ethanol 9:1. Visualization under UV 254nm. The hRF values were 80 for MKT and 71 for LRT. Densitometric absorbance measurement at 260 nm (20 mm/s scanning speed). System suitability was verified by resolution, selectivity, capacity and absence of tailing. The method was validated for linearity range (0.3–3.6 μg/zone for MKT, 0.2–4 µg/zone for LRT), for precision, for reproducibility, for robustness, and for accuracy expressed as average recovery values (100 % overall mean) at different concentrations. The TLC-densitometric method was also found statistically equivalent (Student’s t-test and F-test) to a previously described method (HPLC – spectrophotometry), but was better in terms of environmental and health impacts, using green analytical procedure index (GAPI) and analytical eco-scale (scores based on solvents/reagents, energy consumption, occupational hazard and waste generation). The TLC method was also compared to three (equally “green”) different analytical methods of spectrophotometry (without chromatography): response correlation, absorptivity-centering and LRT-MKT ratio derivatives. The TLC method was more sensitive (LOQ values were 82 ng/zone for MKT, 20 ng/zone for LRT).

      Classification: 5c, 7, 17c, 23d, 23e, 24, 32a, 32c
      130 005
      Multiobjective optimization of microemulsion – thin layer chromatography with image processing as analytical platform for determination of drugs in plasma using desirability functions
      Noura H. ABOU-TALEB*, D. T. EL-SHERBINY, N. M. EL-ENANY, H. I. EL-SUBBAGH (*Medicinal Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt;

      J Chromatogr A, 1619, 460945 (2020). Samples were lamotrigin as standard, or extracted with an oil-in-water microemulsion (10 µL butyl acetate, 4 mL n-butanol, 925 mg sodium dodecyl sulphate, 8.6 mL water) either from patients’ raw plasma (for separation from blood proteins) after spiking, or from commercial tablets dissolved in methanol. TLC on silica gel with a water-in-oil microemulsion of 9 mL butyl acetate, 1 mL n-butanol, 250 mg sodium dodecyl sulphate, 250 µL water. Both optimal microemulsions were predicted using Taguchi orthogonal array and Plackett-Burman design. Evaluation in UV 254 nm, quantification from the digital picture using four image processing software programs. For lamotrigin (hRF 24), limits of quantification were 170 ng for pure drug and 10 ng for spiked plasma. Linearity (in range 20–200 ng/spot) was directly obtained for the calibration curve in spiked plasma; however, for pure drug, linearity was obtained only when using log values of the calculated densities (300–3000 ng/spot).

      Classification: 3a, 3d, 5c, 23e, 32c
      129 060
      Detection of low levels of genotoxic compounds in food contact materials using an alternative HPTLC-SOS-Umu-C assay
      (*Institute of Nutritional Science, Justus Liebig University Giessen, and TransMIT Center of Effect-Directed Analysis, Giessen, Germany;

      ALTEX - Alternatives to animal experimentation, 38(3), 387-397 (2021). Samples were standards of food contact contaminants with genotoxicity (4-nitroquinoline-1-oxide (NQO), aflatoxin B1, hexachloroethane, nitroso-ethylurea, phenformin, PhIP) or negative controls (alosetron, mannitol), and extracts of coated tin cans (extracted with n-hexane – acetone at 25°C for 16 h or by heating at 60 °C with ethanol 95 % for 240 h). HPTLC on RP18W layer, pretreated to harden the binder by heating 1 h at 120 °C, prewashed with methanol and with ethyl acetate and dried 4 min in cold air stream after each development. Application areas were focused to their upper edges by a two-fold elution with ethyl acetate, followed by 1 min drying in cold air stream. Development with toluene – ethyl acetate 8:5, followed by 5 min drying, neutralization with citrate buffer (pH 12) and 4 min drying. Effect-directed analysis for genotoxicity (SOS response – UMU-C test, using NQO as positive control) by immersion (speed 3.5 cm/s, time 3 s) into Salmonella typhimurium suspension and, after 3 h incubation at 37 °C and 4 min drying in cold air stream, into one of two fluorogenic substrate solutions (methylumbelliferyl- vs. resorufin-galactopyranoside). After 1 h incubation at 37 °C, visualization of mutagenic compounds as (blue vs. red) fluorescent zones at FLD 366 nm, and densitometry performed with mercury lamp for fluorescence (at  366 / >400nm vs. 550 / >580 nm, respectively). Further validation experiments, including spiking extracts with NQO, were performed showing good mean reproducibility, no quenching or other matrix effects. Lowest effective concentration of NQO was 0.53 nM (20 pg/band), 176 times lower than in the corresponding microtiter plate assays.

      Classification: 4e, 5c, 8b, 16, 23d, 23e, 32d
      106 021
      Development and validation of stability indicating HPTLC method for determination of prasugrel
      T. BOROLE*, R. MEHENDRE, M. DAMLE, K. BOTHARA (*Dept. of Pharmaceutical Chemistry, AISSMS College of Pharmacy, Pune, Maharashtra, India)

      Journal of Chemical and Pharmaceutical Research 2(4), 907-913 (2010). TLC on silica gel with dichloromethane - methanol 99:1. The hRf value of prasugrel was 58±3. Prasugrel was subjected to stress test conditions like acid, alkali, neutral hydrolysis, oxidation, dry heat, and photo degradation. The zones corresponding to degradation products were well resolved from the main drug. Densitometric evaluation in absorbance mode at 254 nm. Linearity was in the range of 300-1500 ng/band.

      Classification: 5c
      106 022
      HPTLC method development and validation quantification of paliperidone in formulations and in vitro release study
      R. PATEL*, Mrunali PATEL, K. BHATT, B. PATEL (*A. R. College of Pharmacy and G. H. Patel Institute of Pharmacy, Sardar Patel University, Vallabh Vidyanagar 388120, India,

      Analytical Methods 2, 521-531 (2010). An HPTLC method for determination for paliperidone in formulation as well as for in vitro release studies has been developed. HPTLC on silica gel with methanol - ethyl acetate 4:1. The hRf value was 54. Quantification was performed by densitometric evaluation at 254 nm. The method was linear in the range of 100-600 ng/band. The method was suitable for estimation of the drug in mucoadhesive microemulsion formulations, as well as for solubility and diffusion studies.

      Classification: 5c
      106 023
      Application of TLC-densitometry method for estimation of acebrophylline in pharmaceutical dosage forms
      W. SOLOMON*, M. MANU, R. SIVAKUMAR, P. ANAND, R. VENKATANARAYANAN (*Dept. of Pharmaceutical Analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore, T.N., India,

      Journal of Pharmacy Research 3(11) (2010). TLC on silica gel (plates pre-washed with methanol) with chloroform - isopropanol - toluene 8:1:1. The hRf value was 22. Densitometric evaluation at 254 nm. The method was linear in the range of 100-5000 ng/band. The recovery was in the range of 99.8-101.5 %.

      Classification: 5c
      106 129
      HPTLC determination of amoxicillin trihydrate and bromhexine hydrochloride in oral solid dosage forms
      M. DHOKA*, V. GAWANDE, P. JOSHI (*Dept. of Q.A. AISSMS College of Pharmacy, Kennedy Rd., near R.T.O. Pune 411001, M.S., India )

      J. Pharma. Sci. & Res. 2(8), 477-483 (2010). TLC on silica gel with ethyl acetate - glacial acetic acid - methanol - water 10:5:3:2. The hRf value was 51 and 74 for amoxicillin and bromhexine, respectively. Densitometric evaluation at 260 nm (bromhexine) and at 320 nm (amoxicillin). The method was linear in the range of 200-1000 ng/band and 10-30 µg/band for bromhexine and amoxicillin, respectively. The recovery was between 98.1-101.5 %.

      Classification: 5c, 28a