ALTEX - Alternatives to animal experimentation, 38(3), 387-397 (2021). Samples were standards of food contact contaminants with genotoxicity (4-nitroquinoline-1-oxide (NQO), aflatoxin B1, hexachloroethane, nitroso-ethylurea, phenformin, PhIP) or negative controls (alosetron, mannitol), and extracts of coated tin cans (extracted with n-hexane – acetone at 25°C for 16 h or by heating at 60 °C with ethanol 95 % for 240 h). HPTLC on RP18W layer, pretreated to harden the binder by heating 1 h at 120 °C, prewashed with methanol and with ethyl acetate and dried 4 min in cold air stream after each development. Application areas were focused to their upper edges by a two-fold elution with ethyl acetate, followed by 1 min drying in cold air stream. Development with toluene – ethyl acetate 8:5, followed by 5 min drying, neutralization with citrate buffer (pH 12) and 4 min drying. Effect-directed analysis for genotoxicity (SOS response – UMU-C test, using NQO as positive control) by immersion (speed 3.5 cm/s, time 3 s) into Salmonella typhimurium suspension and, after 3 h incubation at 37 °C and 4 min drying in cold air stream, into one of two fluorogenic substrate solutions (methylumbelliferyl- vs. resorufin-galactopyranoside). After 1 h incubation at 37 °C, visualization of mutagenic compounds as (blue vs. red) fluorescent zones at FLD 366 nm, and densitometry performed with mercury lamp for fluorescence (at  366 / >400nm vs. 550 / >580 nm, respectively). Further validation experiments, including spiking extracts with NQO, were performed showing good mean reproducibility, no quenching or other matrix effects. Lowest effective concentration of NQO was 0.53 nM (20 pg/band), 176 times lower than in the corresponding microtiter plate assays.

Acta Chromatographica 6, 1-4 (1996). TLC of TNT (trotyl) on silica gel with hexane-benzene 1:1. Detection by spraying with phenol red, bromophenol blue, thymol blue, and bromothymol blue, separately, followed by heating at 100 °C for 10 min. These solutions were prepared immediately before spraying of the plates. The hRf value was 50 (± 2). Linearity was between 2.5 and 10 µg/zone. The correlation coefficient was 0.931. LOD (in average, n = 6) was 1.0 µg/zone (phenol red), 1.0 µg/zone (bromophenol blue), 1.5 µg/zone (thymol blue) and 1.5 µg/zone (bromothymol blue).

J. Planar Chromatogr. 8, 78-80 (1995). HPTLC of nitroimidazoles (dimetridazole, metronidazole, ronidazole) in poultry on silica with ethyl acetate. Detection with fluorescamine (detection limit 15 - 20 ng) and by spraying with pyridine, waiting for 1 min and illumination under UV 366 nm (detection limit 3 - 25 ng).

Braz. J. Microbiol. 46, 103-111 (2015). TLC of 2,4,6-trinitrotoluene (1) and two degradation intermediates with nitrite release into the medium by Pseudomonas aeruginosa, 2,4-dinitrotoluene (2) and 4-aminodinitrotoluene (3) on silica gel with ethyl acetate - hexane 2:3. Identification under UV light at 254 nm. The hRF values for (1) to (3) were 83, 67 and 50, respectively.

J. Planar Chromatogr. 7, 461-463 (1994). TLC on silica with benzene - ethyl acetate 10:1. Detection under daylight and UV 254 nm.

J. High Resol. Chromatogr. 6, 326-328 (1983). TLC of fluorene,2 -nitrofluorene, 2,7-dinitrofluorene, 2,5-dinitrofluorene, 2,4,7-trinitrofluorene, 2,4,5,7-tetranitrofluorene and their -9-ones on a)silica with ethyl acetate - CCl4 1:8 and b) on alumina with chloroform - cyclohexane 2:1 and for both four other solvent systems. Detection by UV

Acta Chromatographica No. 7, 159-171 (1997). TLC of 9 nitrophenones, including isomers and closely related compounds, and reduced derivatives and corresponding alcohols on silica, alumina, and Florisil, with mixtures of ethyl acetate or 2-propanol and heptane. Detection under UV 254 nm. Study of the effect of reduction on the separation of the investigated compounds and on the selectivity of the separation of both groups of substances on various polar sorbents.

J. High Resol. Chromatogr. 7, 520-524 (1984). TLC of 1-nitropyrene after pre-chromatographic reduction as 1-aminopyrene on silica, impregnated with a mixture of 2,6-di-tert.butyl-4-methylphenol and Faublin H-Vac. with hexane - anisole 7:3. Quantitative scanning by fluorescence. Fluorometry.