Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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      129 060
      Detection of low levels of genotoxic compounds in food contact materials using an alternative HPTLC-SOS-Umu-C assay
      (*Institute of Nutritional Science, Justus Liebig University Giessen, and TransMIT Center of Effect-Directed Analysis, Giessen, Germany;

      ALTEX - Alternatives to animal experimentation, 38(3), 387-397 (2021). Samples were standards of food contact contaminants with genotoxicity (4-nitroquinoline-1-oxide (NQO), aflatoxin B1, hexachloroethane, nitroso-ethylurea, phenformin, PhIP) or negative controls (alosetron, mannitol), and extracts of coated tin cans (extracted with n-hexane – acetone at 25°C for 16 h or by heating at 60 °C with ethanol 95 % for 240 h). HPTLC on RP18W layer, pretreated to harden the binder by heating 1 h at 120 °C, prewashed with methanol and with ethyl acetate and dried 4 min in cold air stream after each development. Application areas were focused to their upper edges by a two-fold elution with ethyl acetate, followed by 1 min drying in cold air stream. Development with toluene – ethyl acetate 8:5, followed by 5 min drying, neutralization with citrate buffer (pH 12) and 4 min drying. Effect-directed analysis for genotoxicity (SOS response – UMU-C test, using NQO as positive control) by immersion (speed 3.5 cm/s, time 3 s) into Salmonella typhimurium suspension and, after 3 h incubation at 37 °C and 4 min drying in cold air stream, into one of two fluorogenic substrate solutions (methylumbelliferyl- vs. resorufin-galactopyranoside). After 1 h incubation at 37 °C, visualization of mutagenic compounds as (blue vs. red) fluorescent zones at FLD 366 nm, and densitometry performed with mercury lamp for fluorescence (at  366 / >400nm vs. 550 / >580 nm, respectively). Further validation experiments, including spiking extracts with NQO, were performed showing good mean reproducibility, no quenching or other matrix effects. Lowest effective concentration of NQO was 0.53 nM (20 pg/band), 176 times lower than in the corresponding microtiter plate assays.

      Classification: 4e, 5c, 8b, 16, 23d, 23e, 32d
      103 053
      Detection and determination of trotyl by HPTLC
      T. WIDLA*, M. SLIWIOK (*Faculty of Law and Administration, Department of Criminalistics, Silesian University, 40-006 Katowice, Bankowa Street, Poland)

      Acta Chromatographica 6, 1-4 (1996). TLC of TNT (trotyl) on silica gel with hexane-benzene 1:1. Detection by spraying with phenol red, bromophenol blue, thymol blue, and bromothymol blue, separately, followed by heating at 100 °C for 10 min. These solutions were prepared immediately before spraying of the plates. The hRf value was 50 (± 2). Linearity was between 2.5 and 10 µg/zone. The correlation coefficient was 0.931. LOD (in average, n = 6) was 1.0 µg/zone (phenol red), 1.0 µg/zone (bromophenol blue), 1.5 µg/zone (thymol blue) and 1.5 µg/zone (bromothymol blue).

      Classification: 16
      75 085
      A specific and sensitive fluorescent detection method for the planar chromatographic analysis of nitroimidazoles
      M. GAUGAIN*, J.-P. ABJEAN, (Center. Nat. d’Etudes Vétérinaires et Alimentaires, Laboratoire des Médicaments Vétérinaires, La Haute Marche, Javené - 35133 Fougeres, France)

      J. Planar Chromatogr. 8, 78-80 (1995). HPTLC of nitroimidazoles (dimetridazole, metronidazole, ronidazole) in poultry on silica with ethyl acetate. Detection with fluorescamine (detection limit 15 - 20 ng) and by spraying with pyridine, waiting for 1 min and illumination under UV 366 nm (detection limit 3 - 25 ng).

      Classification: 16, 32d
      116 054
      Degradation of 2,4,6-trinitrotoluene by Pseudomonas aeruginosa and characterization of some metabolites
      H. MERCIMEK*, S. DINCER, G. GUZELDAG, A. OZSAVLI, F. MATYAR, A. ARKUT, F. KAYIS, M. OZDENEFE (*Department of Molecular Biology and Genetics, The Faculty of Sciences and Letters, Kilis 7 Aralik University, Kilis, Turkey,

      Braz. J. Microbiol. 46, 103-111 (2015). TLC of 2,4,6-trinitrotoluene (1) and two degradation intermediates with nitrite release into the medium by Pseudomonas aeruginosa, 2,4-dinitrotoluene (2) and 4-aminodinitrotoluene (3) on silica gel with ethyl acetate - hexane 2:3. Identification under UV light at 254 nm. The hRF values for (1) to (3) were 83, 67 and 50, respectively.

      Classification: 16
      75 086
      Identification of 2,4,6-trinitrotoluene and its biodegradation products by thin-layer chromatography
      Z. HANFA*, Z. SHIFEN, H. XIN, Z. YUKUI, L. PEICHANG, (*National Chromatographic R&A Center, Dalian Inst. of Chem. Phys., Acad. Sinica, Dalian 116011, P.R. China)

      J. Planar Chromatogr. 7, 461-463 (1994). TLC on silica with benzene - ethyl acetate 10:1. Detection under daylight and UV 254 nm.

      Classification: 16, 35c
      52 077
      Thin-layer chromatography of nitrofluorenes and nitrofluoren-9-ones prepared by nitration

      J. High Resol. Chromatogr. 6, 326-328 (1983). TLC of fluorene,2 -nitrofluorene, 2,7-dinitrofluorene, 2,5-dinitrofluorene, 2,4,7-trinitrofluorene, 2,4,5,7-tetranitrofluorene and their -9-ones on a)silica with ethyl acetate - CCl4 1:8 and b) on alumina with chloroform - cyclohexane 2:1 and for both four other solvent systems. Detection by UV

      Classification: 16
      80 052
      Chromatographic separation of nitrophenones and their reduced derivatives on thin layers of polar adsorbents
      M. WAKSMUNDZKA-HAJNOS, (Dept. of Inorg. and Anal. Chem., Med. Acad., 20-081 Lublin, Poland)

      Acta Chromatographica No. 7, 159-171 (1997). TLC of 9 nitrophenones, including isomers and closely related compounds, and reduced derivatives and corresponding alcohols on silica, alumina, and Florisil, with mixtures of ethyl acetate or 2-propanol and heptane. Detection under UV 254 nm. Study of the effect of reduction on the separation of the investigated compounds and on the selectivity of the separation of both groups of substances on various polar sorbents.

      Classification: 16
      55 060
      Determination of 1-nitropyrene by thin-layer chromatography in a diesel exhaust particulate extract
      K. BROWN, C. POOLE

      J. High Resol. Chromatogr. 7, 520-524 (1984). TLC of 1-nitropyrene after pre-chromatographic reduction as 1-aminopyrene on silica, impregnated with a mixture of 2,6-di-tert.butyl-4-methylphenol and Faublin H-Vac. with hexane - anisole 7:3. Quantitative scanning by fluorescence. Fluorometry.

      Classification: 16