A new versatile technique of forced flow planar chromatography. Proc. 6th Int. Symp. Instrum. Planar Chromatogr., (Interlaken 1991), Inst. Chromatogr., Bad Dürkheim, FRG, 61-69 (1991). A new technique of a Forced Flow Planar Chromatography was described. Normal precoated HPTLC silica gel glass plates were covered with a solvent resistant polyethylene (PE) - membrane and developed by vacuum in a special chromatographic apparatus.

Chromatogr. Sci. 55, 283-315 (1991). A review with 68 references on preparative TLC for separation, analysis, determination of biological activity, and structure elucidation.

J. Planar Chromatogr. 6, 13-20 (1993). The chromatographic process on completely covered and pressurized chromatoplates as used in OPLC, theoretically and practically resembles a planar version of high performance column liquid chromatography. The interaction between the sorbent surface and the coversheet (generally plastic) has been demonstrated with electron micrographs.

1. Effect of temperature on Rf values.) (Japanese). Kagaku to Kyoiku (Jap. J. Educ.) 42, 206-210 (1994). Examination of the effects of temperature on Rf values in TLC, with some dyes and simple organic compounds, e.g. nitrobenzene, phenol, etc., and steroids as analytes.

J. Chem. Educ. 73, 77 (1996). Description of a novel TLC developing chamber that greatly enhanced the efficiency of screening column chromatographic fractions in laboratory.

J. Liqu. Chromatogr. 23, 531-549 (2000). Description of the basic features of planar displacement chromatography, including differences from elution chromatography, and that of column displacement chromatography, the possibility of using spacer displacement chromatography. Parameters limiting displacement chromatography, and their optimization to achieve better separation are discussed. Displacement thin-layer chromatography has the advantage of planar arrangement of the stationary phase, and opens new possibilities for analytical separations.

J. Planar Chromatogr. 16, 176-182 (2003). A commercially available horizontal DS chamber has been applied to the planar electrochromatographic separation of a dye mixture and of the enantiomers of phenylalanine on non-polar stationary phases with ethanol-buffer or acetonitrile-buffer as mobile phases. The mobile phases were driven electroosmotically by applying a 50-250 V/cm field along a 10 cm RP-18 plate. Compared with conventional TLC separations the efficiency of electrochromatography was higher and the development time shorter. Conventional HPTLC of a dye mixture (1-aminoanthraquinone, fat brown, 4-diethylaminoazobenzene, 4-hydroxybenzeneazonaphthol-2, 4-(4-(N,N-ethylethanol)benzeneazo)-N-methylphthalimide, 4-nitroaniline) on RP-18 with 80% ethanol in pH 10 buffer (diluted 1:3 with water) and of DL-phenylalanine on CHIR plates in a horizontal chamber after presaturation; detection of DL-phenylalanine by application of ninhydrin solution before densitometry. Densitometry at 440 nm for the dye test mixture and at 520 nm for DL-phenylalanine.

J. Liq. Chrom. Rel. Technol. 27, 1105-1113 (2004). Two-dimensional (2D) TLC has been mostly used for the separation of a large number of compounds that cannot be separated in a single dimension TLC experiment. In 2D TLC, separation is on one surface spread along the entire area of the plate. The resolving power of the 2D TLC has great application, especially in the areas of biochemistry, biology, natural products, pharmaceuticals, and environmental analysis. Detailed description of two-dimensional development, two-dimensional development on bilayers, two-dimensional separation by TLC/electrophoresis, and chromatogram evaluation.