Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      129 071
      Comparison of high-performance thin-layer with overpressured layer chromatography combined with direct analysis in real time mass spectrometry and direct bioautography for tansy root
      Ágnes M. MÓRICZ*, T.T. HÄBE, P.G. OTT, G.E. MORLOCK
      (*Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, 1022 Budapest, Hungary;

      J Chromatogr A, 1603, 355–360 (2019). Samples were ethyl acetate root macerates of fully flowered Tanacetum vulgare (Asteraceae). HPTLC on silica gel (classical irregular particles vs. Lichrosphere with spherical particles) previously washed with methanol, dried for 5 min at room temperature, perimeter-sealed with a polymer coat, and heated for 30 min at 100 °C. Separation with toluene or with toluene – n-hexane 7:3, in classical capillary flow or in OPLC (overpressured layer chromatography). For OPLC, off-line infusion was used (closed mobile phase (MP) outlet, automatically stopping development); external pressure 50 bar, rapid MP flush 175 and 350 µL, MP flow rate 250 and 500 µL/min, 1830 and 3475 µL MP, development time 446 s and 424 s. Derivatization by immersion into vanillin – sulfuric acid reagent, followed by 5 min heating at 110 °C; or into PABA reagent (500 mg p-aminobenzoic acid, 18 mL glacial acetic acid diluted, 20 mL water, 1 mL o-phosphoric acid, 60 mL acetone), followed by 5 min heating at 140 °C. Effect-directed analysis using automated immersion: A) for free radical (DPPH•) scavengers; B) for activity against Gram-negative bacteria using Aliivibrio fischeri bioluminescence assay; C) for activity against Gram-positive bacteria with Bacillus subtilis bioassay. Four active polyynes were identified as hexadiynylidene-epoxy-dioxaspiro-decane (1), pontica epoxyde (nonene-triynyl-vinyl-oxirane) (2), tetradeca-triine-en-one (3) and trans-(hexadiynylidene)-dioxaspiro-decene (4), by hyphenating OPLC to quadrupole-orbitrap HRMS without eluent, using a DART interface (Direct Analysis in Real-Time, needle voltage 4kV, grid voltage 50 V, helium as gas, temperature 500 °C, full scan in positive ionization mode in m/z range 100-750). Polyynes (3) and (4) were coeluting in HPTLC but not in OPLC, demonstrating that (4) is not produced by oxidation during the DART-MS procedure. Separation with OPLC compared to HPTLC was performed in a shorter time and with better resolution at the same time. Layers with spherical particles gave higher resolution; zone distortions occurring in OPLC due to dissolved air in MP were prevented by previous MP sonication.

      Classification: 3b, 3d, 4e, 5a, 8b, 9, 32e
      125 031
      Impregnated silica-based layers in thin layer chromatography
      Anna SOBANSKA (Department of Analytical Chemistry, Faculty of Pharmacy, Medical University of Lodz, Muszynskiego 1, Lodz 90-151, Poland,

      J. Liq. Chromatogr. Relat. Technol. 43, 319-327 (2020). Review of impregnated agents used in TLC and their applications in analytical and medicinal chemistry. Impregnation with inorganic ions, chelating reagents, lipophilic substances, surfactants, chiral selectors and ionic liquids were discussed.

      Classification: 1b, 3b
      124 053
      Highly-porous diatom biosilica stationary phase for thin-layer chromatography
      J.A. KRAAI, G.L. RORRER*, A.X. WANG (*School of Chemical, Biological and Environmental Engineering, Oregon State University, Corvallis, OR 97331, USA,

      J. of Chromatogr. A 1591, 162-170 (2019). TLC of malachite green (1) and fast green (2) on diatom biosilica (a nanostructured, porous stationary phase composed of randomly-deposited biosilica frustules isolated from living cells of diatom Pinnularia sp.) instead of conventional silica gel with 1-butanol - ethanol - water 9:1:1 and 1-butanol - acetic acid - water 5:1:2. Diatom biosilica reduced the flow velocity and permeability constant by a factor two compared to silica gel and thus improved the resolution of (1) and (2). The theoretical plate height for both analytes was reduced ten-fold with 1-butanol - acetic acid - water 5:1:2, and the difference in retention time between malachite green and fast green was increased (ΔhRF 26) with 1-butanol - ethanol - water 9:1:1.

      Classification: 3b
      111 011
      Introduction of special HPTLC and TLC plates for coupling with mass spectrometry
      M. SCHULZ*, B. SCHUBACH, Susanne MINARIK, H. GRIESINGER (*Merck KGaA, MM-LER-CP. Frankfurterstr. 250, 64293 Darmstadt, Germany,

      CBS 110, 10-11 (2013). New HPTLC silica gel plates specially suited for coupling with MS and TLC plates for coupling with NMR. These MS-grade HPTLC plates are low in impurities and show less background noise, the layer thickness is 100 µm. HPTLC of human insulin and its secondary components on MS-grade silica gel with 2-butanol - pyridine - ammonia 25 % - water 39:34:10:26, detection under UV 366 nm after treatment with fluorescamine and by elution with the TLC-MS Interface and Q-TOF MS analysis in ESI positive mode, the eluent was acetonitrile - water 19:1. Separation and identification of insulin and desamido insulin was achieved, even though there is only 1 Da mass difference.

      Keywords: HPTLC
      Classification: 3b, 19
      122 019
      Monolithic thin-layer chromatography plates with covalently bonded matrix for hyphenation with matrix-assisted laser
      E. KUCHERENKO, Anastasiia KANATEVA*, A. KURGANOV, R. BORISOV, A. PIROGOV (*Institute of Petrochemical Synthesis, Russian Academy of Sciences, Lenin av., 29, 119991 Moscow, Russia,

      J. Sep. Sci. 41, 4387-4393 (2018). A new separation layer for TLC plates was developed for the separation in thin layers and direct conjugation to matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) without additional sample preparation. Best results were obtained with layers containing 19 to 29 % glycidyl methacrylate in the initial polymerization mixture.

      Keywords: HPTLC
      Classification: 3b, 4e
      59 018
      Duennschicht-chromatographische Enantiomerentrennung mittels Ligandenaustausch
      K. GÜNTHER

      (Thin-layer chromatographic enantiomeric resolution via ligand exchange.) GIT-Suppl. 3, 6-12 (1986). Separation of enantiomeric amino acids and dipeptides on reversed phase silica gel covered with a chiral selector (proline derivative). Typical developing solvents: methanol - water - acetonitrile 5:5:20 (running time 30 min) and 5:5:3 (running time 90 min).

      Classification: 3b, 18a, 18b
      60 110
      Thin-layer chromatography of some methylated amino acids with aqueous salt solutions used as eluents
      T. CSERHATI*, B. BORDAS, E. TYIHAK, (*Plant Prot. Inst. Hung. Acad. Sci., Herman O.15, H-1022 Budapest)

      J. Chromatogr. 365, 289-296 (1986). TLC of methylated lysine and arginine derivatives on various silicas with various aqueous salt solutions. Investigation of the influence of the origin of silica, impregnation and salt quality on chromatographic performance. The origin of the silica has a dominant influence on chromatographic performance, and the effect of impregnation and salt quality are of secondary importance.

      Classification: 3b, 18a
      65 023
      Optimization of separation of lypophylic fractions of hydrobionts by thin-layer chromatography
      E.S. GANKINA*, L.S. LITVINOVA, N.A. PISAREVA, M.S. ZHAKEVICH, L.R. KOPYLENKO, (*Inst. Macromolecular Compounds of the Academy of Sciences, Leningrad 199004, USSR,)

      J. Planar Chromatogr. 2, 158-160 (1989). TLC of lypophilic fraction of hydrobionts on different commercial plates with various physical characteristics. For hydrocarbons (hexadecylbenzene, dimethyl naphthalene, phenanthrene and 3,4 a-benzopyrene) the selectivity does not change with the grain diameter of the sorbent (5 - 25 µm). The Rf-value is markedly influenced by chamber saturation and specific surface areas. Plates containing a silicium acid sol as binder may be used 10 - 12 times after regeneration (increase of reproducibility).

      Classification: 3b, 5b, 11c