Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. The saved items can be printed to PDF using the print function of your web browser.

      125 019
      High-performance thin-layer chromatography marker-based standardization of piperine, asiaticoside, and withanolide‑A in the developed polyherbal formulation and in vitro evaluation of acetylcholinesterase inhibition
      V. PAI, K. CHANDRASHEKAR, P. PICHESHWARA, M. SETTY (*Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal ‑ 576 104, Karnataka, India, mm.setty@manipal.edu)

      Pharmacogn. Mag. 15, 256-260 (2019). HPTLC of piperine (1), asiaticoside (2), and withanolide A (3) in a polyherbal formulation of Piper longum, Centella asiatica, and Withania somnifera on silica gel with toluene - ethyl acetate 9:1 for (1), ethyl acetate - methanol - water 20:5:2 for (2) and toluene - ethyl acetate - formic acid 5:5:1 for (3). Detection by spraying with 5 % aqueous sulfuric acid, followed by heating at 110 °C. The developed plates were scanned at 254 nm, 366 nm and visible light. The hRF values for (1) to (3) were 24, 71 and 77, respectively.

      Classification: 14, 22
      125 027
      Metabolic differentiation and quantification of gymnemic acid in Gymnema sylvestre (Retz.) R.Br. ex Sm. leaf extract and its fermented products
      J. KHAN, S. SHEORAN, W. KHAN, B. PANDA* (* Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education & Research, Jamia Hamdard. New Delhi, India, bibhu_panda31@rediffmail.com)

      Phytochem. Anal. 31, 57-67 (2019). HPTLC of gymnemagenin in the leaves of Gymnema sylvestre on silica gel with toluene - chloroform - methanol 5:8:3. Detection by spraying with vanillin sulphuric acid reagent, followed by heating at 110 ºC for 5 min. Quantitative determination by absorbance measurement at 610 nm. The hRF value for gymnemagenin was 37. Linearity was between 400 and 3000 ng. The LOD and LOQ were 68 and 206 ng, respectively.

      Classification: 14
      125 037
      Comprehensive HPTLC fingerprinting: A novel economic approach to evaluating the quality of Ganoderma lucidum fruiting body
      Débora FROMMENWILER*, D. TREFZER, M. SCHMID, S. CAÑIGUERAL, E. REICH (*Unit of Pharmacology, Pharmacognosy and Therapeutics, Faculty of Pharmacy and Food Sciences, University of Barcelona, Av. Joan XXIII, 27-3, ES-08028 Barcelona, Spain, debora.frommenwiler@camag.com)

      J. Liq. Chromatogr. Relat. Technol. 43, 414-423 (2020). HPTLC fingerprint of Ganoderma lucidum fruiting body on silica gel with toluene - tetrahydrofuran - acetic acid 70:30:1. Detection by dipping into a solution of 10% sulfuric acid in methanol, followed by heating at 100 ºC for 3 min. Qualitative determination under UV light at 254 and 366 nm. The hRF values for ganoderic acids D, B, A, G and C2 were in the zone between 10 and 50. This zone was used for quantification of total triterpene acids by absorbance measurement at 260 nm. Linearity of each of the main peaks between hRF 10 to 50 was determined. The linear range of ganoderic acid A was between 200 and 500 ng/zone.  The study proposes a new method for evaluation, based on “comprehensive high-performance thin layer chromatography (HPTLC) fingerprinting.” Instead of several different methods using different chromatographic techniques, a single HPTLC analysis for identification of Ganoderma lucidum fruiting body with a test for adulteration and quantitative determination of the content of total triterpene acids is proposed. As an alternative to the current tests in the  USP monograph on G. lucidum fruiting body this HPTLC method is a single, low-cost test, which eliminates the UHPLC assay of total triterpene acids.

      Classification: 3g, 14
      124 059
      Simultaneous high-performance thin-layer chromatographic quantification of withaferin A and withanolide A in Solanum nigrum L. “Black Nightshade”
      R. PREET*, R. GUPTA, M. KAUR (*Department of Botany, Punjabi University, Patiala, India, ramanbrar247@gmail.com)

      J. Planar Chromatogr. 32, 339-342 (2019). HPTLC of withaferin A (1) and withanolide A (2) in Solanum nigrum on silica gel with toluene - ethyl acetate - formic acid - ethanol 60:30:1:6. Detection by spraying with p-anisaldehyde sulfuric acid (1 mL of p-anisaldehyde solution in 2 mL of concentrated sulfuric acid and 100 mL of acetic acid). Quantitative determination by absorbance measurement at 530 nm. The hRF values for (1) and (2) were 43 and 55, respectively. 

      Classification: 14
      124 002
      Three new abietane-type diterpenoids from Plectranthus africanus and their antibacterial activities
      R.T. NZOGONG, B.K. NGANOU, A.T. TEDONKEU, M.D. AWOUAFACK, M. TENE*, T. ITO, P. TANE, H. MORITA** (*Laboratory of Natural Products Chemistry, Department of Chemistry, Faculty of Science, University of Dschang, Dschang, Cameroon; ** Institute of Natural Medicine, University of Toyama, Toyama, Japan; * mtene2001@yahoo.fr; ** hmorita@inm.u-toyama.ac.jp)

      Planta Medica 84(1), 59-64 (2018). A multi-step fractionation through silica gel column chromatography (CC) of a methanolic extract of Plectranthus africanus (whole plant, Lamiaceae) was monitored through TLC on silica gel with various solvent mixtures (n-hexane or dichloromethane with either acetone or methanol). Zones were detected under UV and further by spraying with sulfuric acid 20 % and heating at 100°C. For each fraction or TLC profile, the authors provide the CC gradient, the optimal proportions of the solvents used for the TLC mobile phase, as well as the RF values of the molecules isolated by this CC method: new abietane-type diterpenoids (plectranthroyleanones A, B, C), betulinic and oleanolic acids, heterosides of apigenin, rhamnetin and sitosterol.

      Keywords: herbal
      Classification: 8a, 14, 15a, 32e
      124 048
      Development and validation of stability indicating high-performance thin-layer chromatographic (HPTLC) method for quantifcation of asiaticoside from Centella asiatica L. and its marketed formulation
      L. PATEL, M. RAVAL, S. PATEL* (*Charusat University, Ramanbhai Patel College of Pharmacy, Department of Pharmaceutical Chemistry and Analysis, Hwy 139, off Nadiad-Petlad Rd, Changa, India, samirpatel.ph@charusat.ac.in)

      J. AOAC Int. 102, 1014-1020 (2019). HPTLC of asiaticoside in Centella asiatica on silica gel with toluene - ethyl acetate - methanol - glacial acetic acid 2:7:3:1. Detection by spraying with anisaldehyde sulfuric acid reagent. Quantitative determination by absorbance measurement at 595 nm. The hRF values for asiaticoside were between 43 and 47 in the standard, plant powder and marketed formulations. Linearity was between 200 and 1800 ng/zone. Intermediate precisions were below 2 % (n=6). The LOD and LOQ were 51 and 155 ng/zone. Recovery rate was between 97.7 and 105.5 %.

      Classification: 14
      124 064
      Simultaneous HPTLC quantification of three caffeoylquinic acids in Pluchea indica leaves and their commercial products in Thailand
      S. CHEWCHIDA, B. VONGSAK* (*Innovative Research Center of Herbs and Natural Products, Faculty of Pharmaceutical Sciences, Burapha University, Chonburi, Thailand, boonyadist@go.buu.ac.th)

      Rev. Bras. Farmacogn. 29, 177-181 (2019). HPTLC of chlorogenic acid (1), 3,4-O-dicaffeoylquinic acid (2), and 3,5-O-dicaffeoylquinic acid (3) in the leaves of Pluchea indica on silica gel with ethyl acetate - water - formic acid - toluene 20:2:2:1. Quantitative determination by absorbance measurement at 326 nm. The hRF values for (1) to (3) were 34, 63 and 79, respectively. Linearity was between 100 and 400 ng/zone for (1) to (3). Intermediate precisions were below 3 % (n=3). The LOD and LOQ were 9.9 and 30.1 ng/zone for (1), 17.6 and 53.3 ng/zone for (2) and 6.7 and 20.2 ng/zone for (3), respectively. Recovery rate was 99.0 % for (1), 97.5 % for (2) and 99.6 % for (3).

      Classification: 14
      124 013
      Secondary metabolites in Arctium lappa L.: variation among plant parts and phenological stages
      N.F. BHATT*, R.C. GUPTA, Y. BANSAL (*Punjabi University, Patiala, Punjab, India, bhattnusrat91@gmail.com)

      J. Planar Chromatogr. 32, 461-465 (2019). HPTLC of betulinic acid (1), oleanolic acid (2) and lupeol (3) on silica gel with benzene - ethyl acetate - formic acid 679:227:94 for (1), n-hexane - ethyl acetate - glacial acetic acid 40:20:1 for (2) and n-hexane - ethyl acetate 4:1 for (3). Detection by spraying with anisaldehyde sulfuric acid, followed by heating at 105-110 ºC for 5-10 min. Quantitative determination by absorbance measurement at 530 nm. The hRF values for (1) to (3) were 79, 46 and 44, respectively. Intermediate precision was below 2 % (n=3).

      Classification: 14