Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      129 067
      Guided isolation of new iridoid glucosides from Anarrhinum pubescens by high-performance thin-layer chromatography – acetylcholinesterase assay
      E. MAHRAN, Gertrud E. MORLOCK*, M. KEUSGEN (*Institute of Nutritional Science, Justus Liebig University Giessen, and TransMIT Center of Effect-Directed Analysis, Giessen, Germany; gertrud.morlock@uni-giessen.de)

      J Chromatogr A, 1609, 460438 (2020). HPTLC of defatted hydro-methanolic extract of Anarrhinum pubescens (= A. duriminium) aerial parts (Plantaginaceae) on silica gel with chloroform – methanol 9:2. When intended for MS experiments, layers were previously washed twice with methanol – water 4:1 and heated 20 min at 110 °C. Derivatization by automatic piezoelectric spraying of anisaldehyde sulfuric acid reagent, followed by heating 4 min at 105 °C. Effect-directed analysis for acetyl-cholinesterase (AChE) inhibitors was performed by successive piezoelectric sprayings with TRIS buffer, with AChE solution and (after 30 min incubation at 37 °C) with naphthyl acetate and Fast Blue salt B solution. White inhibiting zones on purple background were documented under white light, and densitometry was measured by scanning in fluorescence mode at 500 nm. One of the active bands was eluted from untreated layer with methanol through the oval elution head of a TLC-MS interface pump, into a quadrupole-Orbitrap mass spectrometer using heated electrospray ionization (HESI); a full scan mass spectrum (m/z 50−750) in the positive ionization mode was recorded, as well as HRMS/MS data across a range of collision energies (10–50 V). The compound was identified as foliamenthoyl-cinnamoyl-antirrhinoside. It was applied with two other active antirrhinosides (iridoids), all isolated from the extract through column chromatography, on an HPTLC layer without migration and submitted to AChE assay; their activity was expressed as equivalency towards rivastigmine tartrate as positive control. 

      Classification: 4e, 7, 8b, 14, 32e
      129 028
      Marker‑based standardization of Terminalia arjuna bark for the detection of probable adulterants by quantitative high‑performance thin‑layer chromatography
      J. TULSI*, A. VIDHU (*Department of Pharmacognosy and Phytochemistry (SEPR), Jamia Hamdard, New Delhi 11062, India, tulsilimbu2016@gmail.com)

      J. Planar Chromatogr. 35, 169-179 (2022). HPTLC of arjungenin (1) and arjunetin (2) in the bark of Terminalia arjuna on silica gel with toluene - ethyl acetate - formic acid 5:4:1. Detection by spraying with 10 % solution of sulfuric acid dissolved in methanol. Quantitative determination by absorbance measurement at 420 and 475 nm for (1) and (2). The hRF values for (1) and (2) were 43 and 67, respectively. Linearity was between 100 and 1000 ng/zone for (1) and (2). The method facilitated the detection of traces amount of adulterants in herbal products.

      Classification: 14
      129 033
      Identification of potential source of quality raw material of Costus speciosus from Western coast of Malabar
      A. SRIVASTAVA, P. RAWAT, M. KUMAR, V. NIRALA, S. SINGH, K. PRABHU, V. SUNDARESAN, S. SRIVASTAVA* (*Pharmacognosy Division, CSIR-National Botanical Research Institute, Lucknow, U.P. 226001, India, sharad_ks2003@yahoo.com)

      J. Planar Chromatogr. 35, 139-151 (2022). HPTLC of diosgenin in Costus speciosus on silica gel with n-hexane - ethyl acetate 7:2. Detection by spraying with anisaldehyde  -sulfuric acid (0.5 mL anisaldehyde in 1 mL of 97 % sulfuric acid), followed by drying at 105 °C. Quantitative determination by absorbance measurement at 440 nm. The hRF value for diosgenin was 23. Linearity was between 0.1 and 0.7 µg/zone. The LOD and LOQ were 0.84 and 2.55 µg/zone. 

      Classification: 14
      129 047
      Stimulation quantification of four natural lipase inhibitors from Alismatis Rhizoma by high‑performance thin‑layer chromatography method
      F. YANG (Yang Fei), M. KIM, L. GU (Gu Lihua), L. LI (Li Linnan), L. YANG (Yang Li), Z. WANG* (Wang Zhentao) (*Shanghai R&D Centre for Standardization of Chinese Medicines, Shanghai, China, ztwang@shutcm.edu.cn)

      J. Planar Chromatogr. 35, 3-12 (2022). HPTLC of alisol B 23-acetate (1), alisol B (2), alisol A (3) and alisol C 23-acetate (4) in the rhizomes of Alisma orientale on silica gel with cyclohexane - ethyl acetate 1:1. The hRF values for (1), (4), (2) and (3) were 62, 42, 28 and 9, respectively Quantitative determination by absorbance measurement at 254 nm for (1) and 208 nm for (2), (3) and (4). Linearity was between 125 and 2000 μg/zone for (1) and 83 and 2000 μg/zone for (2), (3) and (4). Interday and intra-day precisions were below 1 % (n=3). Average recovery was 94.2 % for (1), 90.3 % for (2), 97.0 % for (3) and 90.2 % for (4).

      Classification: 14
      128 091
      A new integrated HPTLC - ATR/FTIR approach in marine algae bioprofiling
      S. AGATONOVIC-KUSTRIN, G. RAMENSKAYA, E. KUSTRIN, D. BABAZADEH ORTAKAND, D.W. MORTON* (*School of Pharmacy and Biomedical Sciences, La Trobe University, Bendigo, Australia; ulrike.grienke@univie.ac.at)

      J. Pharm. Biomed. Anal. 189, 113488 (2020). Various extracts from red alga Plocamium dilatatum (Plocamiaceae), green alga Codium fragile tasmanicum (Codiaceae) and brown algae Carpoglossum confluens (1), Cystophora platylobium (2) and C. retorta (3) (Sargassaceae), Ecklonia radiata (Lessoniaceae), Hormosira banksia (Hormosiraceae), Phyllospora comosa (4) (Seirococcaceae) were separated on HPTLC silica gel with n-hexane – ethyl acetate – acetic acid 70:27:3. Detection A) for antioxidant activity by spraying with methanolic DPPH solution, followed by waiting for 30 min at room temperature; B) for steroids and terpenes with anisaldehyde - sulfuric acid solution, followed by heating for 10 min at 110°C; C) for carbohydrates and polyols with thymol - sulfuric acid, followed by heating for 15-20 min at 120°C. Image-based evaluation in white light and UV 366 nm. The most active bands were also characterized by ATR-FTIR (= attenuated total reflectance – Fourier-transformed infrared) spectroscopy.

      Classification: 10, 13, 14, 15, 24, 32e
      128 003
      Characterization of astragaloside I-IV based on the separation of HPTLC from Pleurotus ostreatus cultivated with Astragalus
      H. LI, Y. ZHAO, W. YANG, Z. ZHANG* (*School of Chemical Engineering and Technology, North University of China, Taiyuan, P. R. China, bdx_sp@163.com)

      J. Food Sci. 85, 3183-3190 (2020). HPTLC of astragalosides standards (I-IV) in Pleurotus ostreatus on silica gel with trichloromethane - methanol - ethyl acetate - water 13:7:2:2. Detection by spraying with 10 % sulfuric acid in ethanol and visualization under IV light at 365 nm. The hRF values for (I) to (IV) were 71, 54, 34 and 31, respectively. Further analysis by mass spectrometry.

      Classification: 14
      128 018
      Evaluation of antioxidant, anti-inflammatory and anticancer activities of diosgenin enriched Paris polyphylla rhizome extract of Indian Himalayan landraces
      D. GUPTA*, S. MISHRA, S. VERMA, A. SHEKHER, H. TAG, P. HUI (*Department of Biotechnology, National Institute of Technology (NIT)-Arunachal Pradesh, Yupia, 791112, Papum Pare, Arunachal Pradesh, India, dasguptadevmalya@gmail.com)

      J. Ethnopharmacol. 270, 113842 (2021). HPTLC of diosgenin in the rhizomes of Paris polyphylla on silica gel with toluene - ethyl acetate 7:3. Detection by spraying with anisaldehyde sulphuric acid reagent, followed by heating at 105 °C for 5 min. Quantitative determination by absorbance measurement at 430 nm. The hRF value of diosgenin was 53.

      Classification: 14
      127 023
      HI-HPTLC-UV/Vis/FLD-HESI-HRMS and bioprofiling of steviol glycosides, steviol, and isosteviol in Stevia leaves and foods
      Gertrud MORLOCK*, J. HEIL (*Institute of Nutritional Science, Chair of Food Science, and TransMIT Center for Effect-Directed Analysis, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany, Gertrud.Morlock@uni-giessen.de)

      Anal. Bioanal. Chem. 412, 6431-6448 (2020). Hydrophilic interaction high-performance
      thin-layer chromatography (HI-HPTLC) of 
      Stevia leaf extracts and 20 different food products on silica gel with acetonitrile - water 4:1. Detection by spraying with 2-naphthol sulfuric acid reagent (2 g in 180 mL ethanol plus dropwise 8 mL 50 % sulfuric acid) followed by heating at 160 ºC for 2 min, primuline reagent (0.1 g in 40 mL water plus 160 mL acetone), followed by documentation at 366 nm or anisaldehyde sulfuric acid reagent (1 mL 4-methoxybenzaldehyde, 8 mL sulfuric acid, 16 mL glacial acetic acid and 140 mL methanol), followed by heating at 110 ºC for 5 min. Quantitative determination by absorbance measurement at 500 nm after derivatization with the 2-naphthol sulfuric acid reagent and at 366/> 400 nm after derivatization with primuline reagent. Full scan mass spectra (m/z 100–1500) were recorded via heated electrospray ionization (HESI) in the positive and negative ionization mode. Bioprofiling and effect-directed detections were performed using a Gram-negative A. fischeri bioassay, Gram-positive B. subtilis bioassay, β-glucuronidase assay, tyrosinase assay, α-glucosidase assay, AChE assay and DPPH assay. 

      Classification: 14