J. Ethnopharmacol. 321, 117566 (2024). HPTLC of friedelin in Euphorbia grantii on silica gel with hexane – ethyl acetate 19:1. Detection by spraying with para anisaldehyde, followed by heating at 105 °C. Quantitative determination by absorbance measurement at 580 nm. LOD and LOQ were 0.5 and 1.8 μg/zone, respectively. 

Phytochem. Anal. 35, 64-76 (2024). HPTLC of specific triterpenoid and phenolic compounds in plant resins on RP-18 with acetonitrile - water 19:1 or methanol - water 7:1. Detection by dipping into cerium-molybdenum reagent, followed by heating. Further analysis by atmospheric pressure chemical ionisation mass spectrometry.

J. Planar Chromatogr. 36, 279-294 (2023). HPTLC of andrographolide (1), gallic acid (2) and quercetin (3) in polyherbal tablets on silica gel with toluene - ethyl acetate - methanol - formic acid 86:60:20:1. Quantitative determination by absorbance measurement at 247 nm. The hRF values for (1) to (3) were 38, 24 and 50, respectively. Linearity was in the range of 400-2400 ng/zone for (1) and 200-1200 ng/zone for (2) and (3). Intermediate precisions were below 1 % (n=3). LOD and LOQ were 48 and 101 ng/zone for (1), 27 and 86 ng/zone for (2) and 19 and 78 ng/zone for (3), respectively. Recovery was between 98.5 and 99.4 %.

J. Planar Chromatogr. 36, 223-235 (2023). HPTLC of stigmasterol in Hygrophila schulli on silica gel with toluene - methanol 9:1. Detection by spraying with anisaldehyde - sulfuric acid reagent, followed y heating at 105 °C for 4-5 min. Quantitative determination by absorbance measurement at 520 nm. The hRF value for stigmasterol was 47. Linearity was in the range of 100-500 ng/zone. Intermediate precisions were below 2 % (n=6). LOD and LOQ were 7 and 21 ng/zone, respectively. Recovery was between 98.9 and 99.2 %.

J. Planar Chromatogr. 36, 111-120 (2023). HPTLC of betulin in Hygrophila schulli on silica gel with toluene - methanol 8:1. Quantitative determination by absorbance measurement at 366 nm. The hRF value for betulin was 49. Linearity was in the range of 200-1000 ng/zone. Intermediate precisions were below 2 % (n=6). LOD and LOQ were 33 and 102 ng/zone, respectively. Average recovery was 98.0 %.

J. Planar Chromatogr. 36, 129-136 (2023). HPTLC of arjunic acid (1), arjungenin (2), arjunetin (3) and arjunglucoside-I (4) in the bark of Terminalia arjuna on silica gel with ethyl acetate - toluene - tetrahydrofuran - acetic acid 10:5:4:1. Detection by spraying with anisaldehyde–sulfuric acid reagent. Quantitative determination by absorbance measurement at 598 nm. The hRF values for (1) to (4) were 64, 39, 24 and 16, respectively. Linearity was in the range of 200-800 ng/zone. Intermediate precisions were below 3 % (n=3). LOD and LOQ were 25 and 75 ng/zone for (1) and (2), 13 and 39 ng/zone for (3) and 13 and 18 ng/zone for (4). Average recoveries for (1) to (4) were 96.2 %, 102.6 %, 98.2 % and 97.9 %, respectively.

Phytochem. Anal. doi:10.1002/pca.3252 (2023). HPTLC of tripterpenes in the roots of Cecropia angustifolia on silica gel with toluene - chloroform - ethanol 4:4:1. Detection by spraying with vanillin–sulfuric acid reagent. Further analysis by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry.

J. Planar Chromatogr. 36, 9-19 (2023). HPTLC of ursolic acid in rat tissues on silica gel and amino phase with toluene - ethyl acetate - methanol 4:1:1. Detection by heating at 120 °C for 3 min, followed by dipping into 5 % liquid paraffin in hexane. Quantitative determination in remission/fluorescence mode at 366 nm. The hRF value for ursolic acid was 60 in normal phase and 50 in amino phase. Linearity was in the range of 96-384 ng/zone. Recovery was between 94.7 and 97.7 %.