Trends Anal. Chem. 149, 116554 (2022). Review of extraction, separation, purification, and identification of Cannabis sativa bioactive compounds. The paper described TLC and HPTLC methods for the analysis of flavonoids, polyphenols, and terpenoids in C. sativa, as well as the characterization of cannabinoids in hemp and cannabis preparations, and its combination with mass spectrometry.

Food Chem. 137, 108790 (2022). HPTLC of ursolic acid and oleanolic acid in Ocimum species on silica gel with 1 % iodine solution in chloroform up to 1.3 cm. Plates were kept for 10 min, followed by developing with hexane - ethyl acetate - methanol 82:18:5. Detection by spraying with 1 % methanolic sulfuric acid. 

Chinese Medicine 10, 13 (2015). Samples were root and rhizome extracts of Panax notoginseng (Araliaceae), either raw or in the form of commercial granules. Standards were ginsenosides Rg1, Rb1, Rd, Re and Rg2, notoginsenoside NR1. TLC on silica gel with chloroform – ethyl acetate – methanol – water 15:40:22:9, followed by 10 min air drying. Derivatization for ginsenosides by immersion into sulfuric acid (10 % in ice cold methanol), followed by 10 min air drying and 5 min heating at 100 °C. Quantification by densitometric fluorescence measurement (deuterium and tungstene lamp, 366 nm). For each standard the linear range was 0.05-1 mg/mL (LOQ comprised between 38 and 431 µg/µL). As NR1 and Re (ratio ca. 2:1) had almost the same hRF, they were quantified together as one substance. Multivariate analysis through hierarchical (HCA) and principal component analyses (PCA) was used to order the samples into two clusters, according to the analyte concentrations, the raw plant extracts being richer than most of the commercial products. This TLC method was compared to quantification through UPLC-PDA (Ultra-performance liquid chromatography with photo diode array), which was more sensitive (LOQ between 10 and 49 µg/µL) but did not allow the separation between Rg1 and Re (ratio ca. 6:1).

Chinese Medicine 7, 12 (2012). TLC of a Soxhlet hydro-ethanolic extract of Trichosanthes lobata leaves (Cucurbitaceae) on silica gel with n-hexane – ethyl acetate 7:3. Derivatization with anisaldehyde – sulfuric acid reagent. The presence of flavonoids, saponins, and tannins was found.

Food Control. 136, 108840 (2022). HPTLC of flavonoids, coumarins, sesquiterpene lactones and phenolic acids in German Chamomile (Matricaria recutita L.) on silica gel with ethyl acetate - methanol - water - acetic acid 200:25:20:1 and ethyl acetate - toluene 2:1. Detection of flavonoids and phenolic acids by spraying with Natural product reagent. Detection of sesquiterpene lactones by spraying with anisaldehyde sulfuric acid reagent. Qualitative analysis under UV light at 366 nm. Principal component analysis (PCA) was used for reducing data dimensionality and representing samples across principal components.

Food Chem. 383, 132597 (2022). HPTLC of protodioscin and escin in Camellia and Fenugreek on RP-18 with methanol - water - formic acid 65:35:1. Detection by spraying with 0.5 % p-anisaldehyde in a sulfuric methanol solution, followed by heating at 150 °C until visualization of zones.

J. Liq. Chromatogr. Relat. Technol. 44, 599-609 (2021). HPTLC of lupeol in Betula alnoides on silica gel with chloroform. Detection by spraying with anisaldehyde sulfuric acid reagent. Quantitative determination by absorbance measurement at 525 nm for lupeol. The hRF value for lupeol was 44. Linearity was between 0.8 and 2.4 µg/zone. Inter-day and intra-day precisions were below 5 % (n=3). The LOD and LOQ were 0.2 and 0.5 µg/zone. Recovery was between 100.5 and 106.8 %.

J Chromatogr A, 1628, 461461 (2020). Samples were hydro-methanolic extracts of 100 genuine saffron samples (Crocus sativus stigmata, Iridaceae) from South Khorasan (SK) and Razavi Khorasan (RK) provinces (Iran), pure or mixed in several proportions with common vegetal adulterants: C. sativus style, Calendula officinalis petals (Asteraceae, Asteroideae), Carthamus tinctorius petals (Asteraceae, Carduoideae), Rubia tinctorum rhizomes (Rubiaceae). Commercial saffron samples (containing artificial adulterants) were also tested. TLC on silica gel with ethyl acetate – methanol – water – acetic acid 66:23:11:1. Evaluation at 254 nm, 366 nm, and 440 nm. Crocin (carotenoid, hRF 38) was used for optimization of extraction (parameters being first calculated by chemometry), using multilinear regression and ANOVA. Image data (pixel intensities and colors of each sample under the three selected wavelengths) were unfolded into a data matrix and transformed into a vector, used for multivariate image analysis of the chromatogram fingerprints. This allowed: A) separation of genuine samples by principal component analysis (PCA) into 2 clusters according to origin (cold climate in Northern half of RK vs. warm climate in SK and Southern part of RK) with 92 % prediction accuracy; B) separation of samples according to purity / vegetal adulterant groups by partial least squares – discriminant analysis (PLS-DA) with 98 % accuracy (if 10 µL extract applied); C) separation with 100 % prediction accuracy by PCA between genuine, mixed, and commercial samples.