Planta Med. 85(2), 154-159 (2019). Two new cycloartane triterpene heterosides (cimiheraclein F and a shengmanol derivative) isolated from Actaea heracleifolia aerial parts (Ranunculaceae) were submitted to acid hydrolysis (HCl 0.4 M in methanol 60 %, 90°C, 2 h). The glycone moieties were purified and developed on TLC silica gel with ethyl acetate – chloroform – methanol – water 3:2:2:1. Detection after spraying with sulfuric acid 10 % in water and heating. The glycones were identified in both cases as L-arabinopyranose by comparison of the RF values (and of specific optical rotation) to a standard.

Quim. Nova. 43, 878-883 (2020). HPTLC of caryophyllene (1) and the diterpenic acids copalic (2), hydroxy-copalic (3), acetoxycopalic (4), agathic (5), and hardwickiic (6) in oilresin of Copaifera on silica gel with hexane - ethyl acetate 7:3. Detection by spraying with ethanol in 10 % sulfuric acid, followed by heating for 5 min. Qualitative analysis at 366 nm. The hRF values for (1) to (6) were 80, 50, 8, 28, 16 and 40, respectively.

J. Planar Chromatogr. 33, 281-291 (2020). HPTLC of Weikangling capsules (a Chinese patent medicine for treating chronic gastritis, containing Paeoniae Radix Alba, Glycyrrhizae Radix et Rhizoma, Bletillae Rhizoma, Notoginseng Radix et Rhizoma, Poria, Corydalis Rhizoma, Sepiae Endoconcha, and Belladonnae Extractum) on silica gel with chloroform - methanol - water 14:5:1. Detection by spraying with 10 % sulfuric acid in alcohol, followed by heating at 105 ºC for 5 min. Qualitative analysis at UV 366 nm.

J. Planar Chromatogr. 33, 353-364 (2020). HPTLC of friedelin (1), methylputranjate (2), putrone (3), roxburghonic acid (4), putranjivadione (5), and roxburghonol (6) in the bark of the plant Putranjiva roxburghii on silica gel with n-hexane - ethyl acetate 9:1. Detection by dipping into perchloric acid reagent (50 mL 70% perchloric acid in 50 mL acetone), followed by heating at 110-120 ºC for 5 min. Quantitative determination by absorbance measurement at 570 nm. The hRF values for (1) to (6) were 79, 46, 36, 31, 25 and 21, respectively. Linearity was between 1 and 5 µg/zone for (1) to (6). Intermediate precision was below 2 % (n=3). The LOD and LOQ were 780 and 2600 ng/zone for (1), 210 and 700 ng/zone for (2), 170 and 570 ng/zone for (3), 350 and 1170 ng/zone for (4), 460 and 1530 ng/zone for (5) and 380 and 1270 ng/zone for (6), respectively. Average recovery was 98.0 % for (1), 99.1 % for (2), 97.6 % for (3), 98.9 % for (4), 98.7 % for both (5) and (6).

J. Planar Chromatogr. 33, 365-370 (2020). HPTLC of lupeol (1) and β-sitosterol (2) in the stem bark of Crataeva nurvala on silica gel with toluene - methanol 24:1. Detection by spraying with 1 % anisaldehyde - sulfuric acid reagent, followed by heating at 110 ºC. Quantitative determination by absorbance measurement at 545 nm. The hRF values for (1) and (2) were 48 and 34, respectively. Linearity was between 2 and 6 µg/zone for both (1) and (2). Intermediate precision was below 2 % (n=3). The LOD and LOQ were 43 and 133 ng/zone for (1) and 57 and 173 ng/zone for (2), respectively. Average recovery was 98.6 % for (1) and 98.1 % for (2).

J. Planar Chromatogr. 33, 321-326 (2020). HPTLC of astragaloside IV (1) and calycosin-7-O-β-glucoside (2) from raw and honey-processed Radix Astragali on silica gel with chloroform - methanol - water 13:7:2. Quantitative determination by absorbance measurement at 365 nm. The hRF values for (1) and (2) were 36 and 68, respectively. Linearity was between 0.25 and 2.05 µg for (1) and 0.073 and 2.94 µg for (2). Intermediate precision was below 2 % (n=3). The LOD and LOQ were 42 and 126 ng for (1) and 15 and 45 ng for (2), respectively. Average recovery was 98.7 % for (1) and 99.1 % for (2).

Pharmacogn. Mag. 15, 256-260 (2019). HPTLC of piperine (1), asiaticoside (2), and withanolide A (3) in a polyherbal formulation of Piper longum, Centella asiatica, and Withania somnifera on silica gel with toluene - ethyl acetate 9:1 for (1), ethyl acetate - methanol - water 20:5:2 for (2) and toluene - ethyl acetate - formic acid 5:5:1 for (3). Detection by spraying with 5 % aqueous sulfuric acid, followed by heating at 110 °C. The developed plates were scanned at 254 nm, 366 nm and visible light. The hR_F values for (1) to (3) were 24, 71 and 77, respectively.

Pharmacogn. Mag. 15, 462-467 (2019). HPTLC of diosgenin in the rhizome of Costus speciosus on silica gel with n‑hexane - ethyl acetate 7:2. Detection by spraying with anisaldehyde‑sulfuric acid reagent. Quantitative determination by absorbance measurement at 440 nm. The hRF value for diosgenin was 23. Linearity was between 10 and 90 ng/zone. Intermediate precisions were below 5 % (n=2). The LOD and LOQ were 907 and 2751 ng/zone, respectively. Mean recovery was 100.3 %.