J. Planar Chromatogr. 12, 456-460 (1999). TLC of OABA, OABA-glc and OABA-di-glc on silica gel with ethyl acetate - methanol - water 7:2:1. Ascending chromatography. Quantitation by densitometry in absorbance-reflectance mode at 332 nm. - The densitometric method is selective, precise, and accurate and can be used for routine analysis. New simple and rapid TLC procedure.

Planta med. 66, 587-590 (2000). TLC of chloromaloside A on silica gel with chloroform - methanol - water 6:4:1. Visualization by spraying with 10% sulfuric acid followed by heating. Also TLC on silica gel RP with methanol - water 7:3.

cultivated in Poland. Proc. Intern. Symp. on Planar Separations Plan. Chrom. 291-296 (2003). TLC, HPTLC and OPLC of ginsenosides (e.g. Rb 1, Rc, Re, Rd, Rg1, and Rg 2) on normal and HPTLC silica gel with chloroform - methanol - ethyl acetate - water 15:22:40:9 (I) and chloroform - methanol - ethyl acetate - water - hexane 10:11:30:4:2 (II). Mobile phase II gave better results for circular and linear forced-flow OPLC. Visualization by spraying with Godin's reagent (5% sulfuric acid in ethanol and 1% vanillin in ethanol) and heating at 105°C for 10 min. Quantitation by densitometry at 540 nm.

Application of SFC in implementation of uniform design for herbal drug standardization, with thermodynamic study. Acta Chrom. 17, 125-150 (2006). HPTLC of bacoside A3 and bacopaside II on RP-18 F254 after pre-washing with methanol and heating at 60° C for 5 min. Development with toluene – methanol – ethyl acetate 15:5:4 in the dark in a controlled humidity chamber (humidity of 55 - 65 %). Densitometry at 344 nm. The method is available for content determination of bacoside A3 and bacopaside II in herbal extracts and commercial formulations.

J. Chromatogr. A 1207 (1-2), 155-159 (2008). HPTLC of secoisolariciresinol diglucoside in flaxseed on RP18W with methanol - 0.1 % formic acid 2:3, using the alkaline hydrolysis in aqueous medium of undefatted samples. Quantitative determination by absorbance measurement at 282 nm. Validation of the method following the protocol proposed by the Société Francaise des Sciences et Techniques Pharmaceutiques lead to a dependable and high throughput procedure well suited for routine application. Linearity was between 321–1071 ng/zone and the RSD of repeatability and intermediate precision did not exceed 3.6 %.

J. Planar Chromatogr. 27, 42-46 (2014). HPTLC of beta-sitosterol in the root cultures of Clitoria ternatea on silica gel with n-hexane - acetone 4:1. Detection by dipping into 5 % methanol - sulfuric acid reagent, followed by heating at 105 ºC for 3 min. Quantitative determination by absorbance measurement at 414 nm. The hRF value for beta-sitosterol was 31. Linearity was in the range of 100-500 ng/zone. The intermediate/interday/intra-day precisions were below 2 % (n=3). The LOD and LOQ were 40 and 100 ng/zone, respectively. Recovery was between 97.2 and 98.5 %.

Food Res. Int. 87, 68-75 (2016). HPTLC of saponins in an extract of Quillaja saponaria on silica gel with chloroform – acetic acid – methanol – water 16:8:3:2. Detection by dipping for 1 s into a matrix solution (2,5-dihydrobenzoic acid 200 mg/mL in acetonitrile – water 9:1 with 0.1 % trifluoroacetic acid 3:7 and 10 mM ammonium dihydrogen phosphate), followed by drying for 90 s under an airstream. Dipping was repeated and the plate was dried for another 4 min. The plate was stored in a vacuum oven at 60 °C ensuring complete desiccation of the matrix coating. The measurement was performed by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF/TOF).

Planta Medica 82(18), 1576-1583 (2016). The fractionation (by column and flash chromatography) of an aqueous extract of the aerial parts of Hilaria hirsuta was monitored through TLC on silica gel with n-butanol – acetic acid – water 13:3:5. The compounds (including flavonoids narcissin and rutin, and two new saponins, herniariasaponins G and H) were detected with anisaldehyde sulfuric acid reagent.