J. Agric. Food Chem. 49, 4278-4283 (2001). TLC of i.a. a-pinitol, sucrose on silica gel with ethyl acetate - 1-propanol - water 1:1:1. Visualization by spraying with an aqueous 1% solution of sodium periodate in 3% acetic acid, and heating for 2 min at 100°C; followed by spraying with a second reagent containing 1 g aniline, 1 g diphenylamine, and 5 mL 85% phosphoric acid in 50 mL acetone. Visualization of sugars with 1-naphthol spray. Also preparative TLC on HPTLC silica gel plates with ethyl acetate - pyridine - water 2: 1: 29 and TLC of pinitol a-D-galactoside on silica gel amino-phase.

Planta med. 68, 822-826 (2002). TLC of ß-amyrin stearate, ß-amyrin eicosanoate, ß-amyrin docosanoate, and erythrodiol fatty acid esters on silica gel with n-hexane - dichloromethane 4:1, and dichloromethane - methanol 19:1. Visualization under UV light at 254 and 366 nm and by spraying with 1 % vanillin - 5 % sulfuric acid reagent followed by heating at 100 °C. Also TLC of xylose and glucose with i-propanol - water 17:3 and detection with p-anisidine phthalic acid reagent.

J. Liq. Chromatogr. Relat. Technol. 29, 219-227 (2006). HPTLC of selected sarsasapogenins (shataverin-IV and immunoside) on silica gel with ethyl acetate - methanol - water 75:13:5:10. Detection by spraying with ceric ammonium sulfate followed by heating at 100 °C for 5 min. Quantitation by scanning at 450 nm.

J. Liq. Chromatogr. Relat. Technol. 34, 1502-1517 (2011). HPTLC of glycyrrhizic acid in bulk drug and pharmaceutical formulations on silica gel with butanol - glacial acetic acid - water 7:1:2. Quantitative determination by absorbance measurement at 254 nm. The hRf of glycyrrhizin was 24. Linearity was 200-1000 ng/zone. Limits of detection and quantification were found to be 80 and 200 ng/band. The intermediate/inter-day/intra-day precision was below 0.6 % (n=3). Recoveries (by standard addition) were 98.1-99.5 %.

J. Planar Chromatogr. 28, 287-293 (2015). HPTLC of bacoside A in the leaves of Bacopa monnieri on silica gel with ethyl acetate - methanol - water 4:1:1. Detection by spraying with 1 % vanillin in 10 % methanolic sulfuric acid. Quantitative determination by absorbance measurement at 598 nm. The hRF value for bacoside A was 53. LOD and LOQ were 60 and 180 ng/zone, respectively. The intermediate precision was below 1 % (n=3). Recovery ranged between 97 and 100 %.

Planta Medica 82, 11/12, 1117-1121 (2016). TLC was applied for two purposes: first, to monitor the subfractionation of the methanol extract of the whole Calamus acanthophyllus (eluent and layer not given); second, to analyze the sugar composition of callaphylloside (a weakly cytotoxic pregnastanol saponoside). For this purpose, callaphylloside was heated for 3 h with HCl 2 N under reflux; TLC of the lyophilized and neutralized ethyl acetate extract of the mixture on silica gel with ethyl acetate – n-butanol – water 2:7:1 together with standards. The sugar moiety consisted in rhamnose and glucose (2 units of each, as confirmed through NMR of the compound)._x000D_

and Glycyrrhiza uralensis Fisch.) by HPTLC, validated by HPLC and DNA sequencing. J. Planar Chromatogr. 30, 467-473 (2017). HPTLC of 18-β-glycyrrhizic acid in two licorice root species (Glycyrrhiza glabra L. and Glycyrrhiza uralensis Fisch) on silica gel with dichloromethane – methanol – water – formic acid 120:75:15:1 with chamber saturation for 20 min to a migration distance of 70 mm. Quantitative determination by absorbance measurement at 254 nm. Detection of species by immersion into sulfuric acid reagent, followed by heating at 100 °C for 10 min, evaluation under UV 366 nm and white light. The HPTLC results correlate with the data obtained by HPLC and by DNA sequencing.

by high-performance thin-layer chromatography method. J. Planar Chromatogr. 31, 337-342 (2018). HPTLC of betulinic acid (1), oleanolic acid (2) and lupeol (3) in Achyranthes aspera on silica gel with benzene – ethyl acetate – formic acid 679:227:94 for (1), n-hexane – ethyl acetate – glacial acetic acid 40:20:1 for (2) and n-hexane – ethyl acetate 4:1 for (3). Detection by spraying with anisaldehyde sulfuric acid reagent for (1) and (2) and ceric ammonium sulfate for (3), followed by heating at 105-110°C for 5-10 min. Quantitative determination by absorbance measurement at 530 nm for (1) and (2) and 366 nm for (3). The hRF values for (1) to (3) were 79, 46 and 44, respectively. Linearity was between 2-10 μL/zone. LOD and LOQ were 1 and 4 ng/zone for (1), 1 and 3 ng/zone for (2), and 2 and 5 ng/zone for (3), respectively.