Hederagenin 3-o-glycosides of the seeds of austral akebia, Akebia trifoliata Var. australis.) (Chinese). Chinese J. Herb Med. (Zhongcaoyao) 25, 171-174 (1994). TLC of triterpenoid saponins of hederagenin 3-o-gycosides on silica with the lower phase of chloroform - methanol -water 160: 5:1 and 13:7:2. Detection by spraying with 50% sulfuric acid in methanol. Identification under UV and by MS, FAB-MS, NMR.

Chinese J. Pharm. Anal. (Yaowu Fenxi Zazhi) 15 (3), 53-54 (1995). TLC of ginsenoside Rg1 on silica with butanol - ethyl acetate - water 4:1:5. Detection by spraying with 10% sulfuric acid in ethanol and heating at 105 ºC for 5 min. Quantification by densitometry at 530 nm.

J. of Natural Products 60, 453-457 (1997). Isolation of new secoiridoid glucosides from an ethanol extract of Jasminum urophyllum by prep. TLC on silica with chloroform - methanol - water 43:37:20. Final purification of the glycosides on RP-18 silica with methanol - water 1:1.

J. Liq. Chrom. & Rel. Technol. 22, 1503-1512 (1999). TLC of solasodine glycosides on silica gel with chloroform - methanol - ammonia 14:5:2. After drying spraying with a blotting solution mixture of isopropanol - methanol - water 1:4:8. It was placed on a stainless steel plate, then covered with a PVDF membrane sheet. After covering with a glass microfiber filter sheet, the plate was pressed evenly for 45 s with a 1308C iron. The PVDF membrane was separated from the plate and dried. - Also TLC of solamargine, determination by spraying with sulfuric acid and Dragendorff reagent.

Planta med. 66, 662-664 (2000). Preparative TLC of 6'-O-palmitoylsitosterol-3-O-b-D-glucoside on silica gel with chloroform - methanol 12:1; 3 x. Detection under UV.

J. Agric. Food Chem. 51,1260-1264 (2003). TLC of glucosides and heterosides, i.a. simmondsin [(7Z)-(1R,3S,4R,5S)-2-(cyano methylene)-3-hydroxy-4,5-dimethoxycyclohexyl b-D-glucopyranoside], on silica gel with chloroform - methanol 4:1. Examination under UV 254 and 366 nm followed by spraying with 1-naphthol reagent. Preparation by adding 10.5 mL of a 15% ethanolic solution of 1-naphthol to a mixture of 40.5 mL ethanol, 4 mL water and 6.5 mL sulfuric acid. After spraying the plates were heated at 100°C for 5 min. Investigation by GC-FID, GC-MS, MS and NMR spectroscopy. New procedure.

J. Planar Chromatogr. 19, 191-194 (2006). TLC of the flavonoid and triterpenoid soyasaponin content (rutin, vitexine, isovitexine, soyasaponin I and VI as standards) on silica gel in a twin-trough chamber with ethyl acetate - formic acid - acetic acid - water 100:11:11:26 for flavonoids. Detection with diphenylboric acid 2-aminoethylester (natural products reagent) followed by PEG reagent. For soyasaponins chloroform - methanol - water 6:4:1 was used. Detection by spraying with anisaldehyde - sulfuric acid reagent followed by heating at 115 °C.

J. Planar Chromatogr. 27, 255-259 (2014). HPTLC of betulin (1) and betulinic acid (2) in Diospyros sylvatica on silica gel with chloroform - methanol - formic acid 46:1:1. Quantitative determination by absorbance measurement at 210 nm. The hRF values for (1) and (2) were 52 and 45, respectively. Linearity was in the range of 400-2200 ng/zone for (1) and (2). The intermediate/interday/intra-day precisions were below 5 % (n=5). The LOD and LOQ were 106 and 352 ng/zone for (1) and 76 and 253 ng/zone for (2), respectively. Average recovery was 95.5 % for (1) and 93.2 % for (2), respectively.