Food Res. Int. 76, 3-10 (2015). HPTLC of saponins in peas (Pisum sativum) on silica gel with chloroform - methanol - water 55:37:8. Detection by dipping into p-anisaldehyde sulfuric acid reagent for 2 s, drying for 10 min, followed by heating at 70 °C for 5 min. Quantitative determination by absorbance measurement at 545 nm. The hRF of soyasaponin βg was 60 as a violet-blue band and for soyasaponin I 57 as an orange band on a dark background. Saponin identification by hyphenation of HPTLC with mass spectrometry (MS) via a TLC–MS interface.

J. Planar Chromatogr. 29, 336-340 (2016). HPTLC of isoalantolactone (1), germacranolide (2), β-sitosterol (3), and lupeol (4) in the roots of Inula cappa on silica gel with toluene ‒ methanol 47:3. Detection by dipping into anisaldehyde reagent followed by heating at 105 ºC until colored bands appeared. Quantitative determination by absorbance measurement at 525 nm. Linearity ranged from 10-70 μg/mL for (1), 10-60 μg/mL for (2), 8-48 μg/mL for (3) and 15-90 μg/mL for (4). The intermediate precisions were below 0.9 % (n=7). The LODs and LOQs were 5 and 10 μg/mL for (1), (2) and (4) and 2 and 6 μg/mL for (3). Average recoveries were between 98.3 and 99.0 % for (1) to (4).

J. Planar Chromatogr. 31, 135-142 (2018). HPTLC of glycyrrhizin (1) and glabridin (2) in the roots, rhizomes and herbal formulations of Glycyrrhiza glabra on RP-18 with methanol – water 7:3. Quantitative determination by absorbance measurement at 256 nm for (1) and 233 nm for (2), respectively. The hRf values for (1) and (2) were 63 and 28, respectively. Linearity was in the range of 1000-7000 ng/zone for (1) and 100-700 ng/zone for (2). The intermediate precision was below 1.3 % (n=6). The LOD and LOQ for (1) and (2) were 8 and 15 ng/zone for (1) and 5 and 18 ng/mL for (2), respectively. Recovery was in the range of 98.9-99.6 % for (1) and 97.3-99.1 % for (2).

J. Chromatogr. 259, 522-526 (1983). TLC of iridoid and secoiridoid glucosides on silica and silanized silica with ethyl acetate - methanol -water 77:15:8. Detection by UV 254 nm or with anisaldehyde-sulfuric acid reagent. Bioassay on the plate by spraying with a suspension of conidial spores of penicillium expansum with or without adding beta-glucosidase. Detection limit of the bioassay 2.5 mg.

Phytochemistry 23, 2505-2508 (1984). TLC of oleanolic acid glycosides on silica with the lower layers of chloroform - methanol - water 12:7:1 or chloroform - methanol - propanol - water 9:10:1:8. Detection with Komarousky reagent, Godin reagent and citrate blood reagent. Separation of oligosaccharide moieties split-off from the saponins by HPTLC on silica with isopropanol - pyridine - water 3:1:1, followed by hydrolysis on the HPTLC plate (by exposure to HCl, 30 min. at 100 °C) and subsequent chromatography in the second direction with chloroform - methanol - water 32:25:5.

J. Agric. Food Chem. 34, 960-963 (1986). TLC on silica with ethyl acetate - water - acetic acid 7:2:2 for saponin preparations, with ethyl acetate - chloroform 4:1 for medicagenic acid and derivatives. HPTLC on silica RP-18 with methanol - water 4:1. Detection by spraying with 5 % ethanolic sulfuric acid and heating for 10 min at 110 °C: Screening by bioassay.

J. Chinese Herb Med. 18, 447-448 (1987) (Zhong Caoyao). TLC on silica with butanol - ethyl acetate - water 4:1:5. Detection by spraying with sulfuric acid - methanol 1:1, heating at 105 °C and observing under UV.

J. Chromatogr. 437, 260-264 (1988). TLC on silica with ethyl acetate - formic acid - water 6:1:1. Detection by spraying with methanolic solution containing aminoethanol diphenylborate and PEG 400. Determination by densitometry at 533 nm.